A Guide to Fluorescent Protein FRET Pairs
Förster or fluorescence resonance energy transfer (FRET) technology and genetically encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live cells with high spatiotemporal resolution. Fluorescent proteins (FPs) are most commonly used as both donor and acceptor fluo...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-09-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/16/9/1488 |
| _version_ | 1798039864579457024 |
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| author | Bryce T. Bajar Emily S. Wang Shu Zhang Michael Z. Lin Jun Chu |
| author_facet | Bryce T. Bajar Emily S. Wang Shu Zhang Michael Z. Lin Jun Chu |
| author_sort | Bryce T. Bajar |
| collection | DOAJ |
| description | Förster or fluorescence resonance energy transfer (FRET) technology and genetically encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live cells with high spatiotemporal resolution. Fluorescent proteins (FPs) are most commonly used as both donor and acceptor fluorophores in FRET biosensors, especially since FPs are genetically encodable and live-cell compatible. In this review, we will provide an overview of methods to measure FRET changes in biological contexts, discuss the palette of FP FRET pairs developed and their relative strengths and weaknesses, and note important factors to consider when using FPs for FRET studies. |
| first_indexed | 2024-04-11T21:59:34Z |
| format | Article |
| id | doaj.art-7d532a5f59e543178b42d5ac2677f606 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T21:59:34Z |
| publishDate | 2016-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-7d532a5f59e543178b42d5ac2677f6062022-12-22T04:01:00ZengMDPI AGSensors1424-82202016-09-01169148810.3390/s16091488s16091488A Guide to Fluorescent Protein FRET PairsBryce T. Bajar0Emily S. Wang1Shu Zhang2Michael Z. Lin3Jun Chu4Medical Scientist Training Program, University of California, Los Angeles, CA 90095, USAHarvard College, Cambridge, MA 02138, USAInstitute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, ChinaDepartments of Bioengineering and Neurobiology, Stanford University, CA 94305, USAInstitute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, ChinaFörster or fluorescence resonance energy transfer (FRET) technology and genetically encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live cells with high spatiotemporal resolution. Fluorescent proteins (FPs) are most commonly used as both donor and acceptor fluorophores in FRET biosensors, especially since FPs are genetically encodable and live-cell compatible. In this review, we will provide an overview of methods to measure FRET changes in biological contexts, discuss the palette of FP FRET pairs developed and their relative strengths and weaknesses, and note important factors to consider when using FPs for FRET studies.http://www.mdpi.com/1424-8220/16/9/1488fluorescence resonance energy transfer (FRET)biosensorsfluorescent proteins |
| spellingShingle | Bryce T. Bajar Emily S. Wang Shu Zhang Michael Z. Lin Jun Chu A Guide to Fluorescent Protein FRET Pairs Sensors fluorescence resonance energy transfer (FRET) biosensors fluorescent proteins |
| title | A Guide to Fluorescent Protein FRET Pairs |
| title_full | A Guide to Fluorescent Protein FRET Pairs |
| title_fullStr | A Guide to Fluorescent Protein FRET Pairs |
| title_full_unstemmed | A Guide to Fluorescent Protein FRET Pairs |
| title_short | A Guide to Fluorescent Protein FRET Pairs |
| title_sort | guide to fluorescent protein fret pairs |
| topic | fluorescence resonance energy transfer (FRET) biosensors fluorescent proteins |
| url | http://www.mdpi.com/1424-8220/16/9/1488 |
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