Brain-wide neuron quantification toolkit reveals strong sexual dimorphism in the evolution of fear memory
Summary: Fear responses are functionally adaptive behaviors that are strengthened as memories. Indeed, detailed knowledge of the neural circuitry modulating fear memory could be the turning point for the comprehension of this emotion and its pathological states. A comprehensive understanding of the...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723009191 |
| _version_ | 1797731094443851776 |
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| author | Alessandra Franceschini Giacomo Mazzamuto Curzio Checcucci Lorenzo Chicchi Duccio Fanelli Irene Costantini Maria Beatrice Passani Bianca Ambrogina Silva Francesco Saverio Pavone Ludovico Silvestri |
| author_facet | Alessandra Franceschini Giacomo Mazzamuto Curzio Checcucci Lorenzo Chicchi Duccio Fanelli Irene Costantini Maria Beatrice Passani Bianca Ambrogina Silva Francesco Saverio Pavone Ludovico Silvestri |
| author_sort | Alessandra Franceschini |
| collection | DOAJ |
| description | Summary: Fear responses are functionally adaptive behaviors that are strengthened as memories. Indeed, detailed knowledge of the neural circuitry modulating fear memory could be the turning point for the comprehension of this emotion and its pathological states. A comprehensive understanding of the circuits mediating memory encoding, consolidation, and retrieval presents the fundamental technological challenge of analyzing activity in the entire brain with single-neuron resolution. In this context, we develop the brain-wide neuron quantification toolkit (BRANT) for mapping whole-brain neuronal activation at micron-scale resolution, combining tissue clearing, high-resolution light-sheet microscopy, and automated image analysis. The robustness and scalability of this method allow us to quantify the evolution of activity patterns across multiple phases of memory in mice. This approach highlights a strong sexual dimorphism in recruited circuits, which has no counterpart in the behavior. The methodology presented here paves the way for a comprehensive characterization of the evolution of fear memory. |
| first_indexed | 2024-03-12T11:53:47Z |
| format | Article |
| id | doaj.art-7d54546cb2c24c4b89a1c7a43a25de8a |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-12T11:53:47Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-7d54546cb2c24c4b89a1c7a43a25de8a2023-08-31T05:02:09ZengElsevierCell Reports2211-12472023-08-01428112908Brain-wide neuron quantification toolkit reveals strong sexual dimorphism in the evolution of fear memoryAlessandra Franceschini0Giacomo Mazzamuto1Curzio Checcucci2Lorenzo Chicchi3Duccio Fanelli4Irene Costantini5Maria Beatrice Passani6Bianca Ambrogina Silva7Francesco Saverio Pavone8Ludovico Silvestri9European Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy; Department of Physics and Astronomy, University of Florence, Sesto Fiorentino, Italy; Corresponding authorEuropean Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy; Department of Physics and Astronomy, University of Florence, Sesto Fiorentino, Italy; National Institute of Optics - National Research Council (CNR-INO), Sesto Fiorentino, ItalyDepartment of Information Engineering (DINFO), University of Florence, Florence, ItalyDepartment of Physics and Astronomy, University of Florence, Sesto Fiorentino, ItalyDepartment of Physics and Astronomy, University of Florence, Sesto Fiorentino, ItalyEuropean Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy; Department of Biology, University of Florence, Florence, ItalyDepartment of Health Sciences, University of Florence, Florence, ItalyNational Research Council of Italy, Institute of Neuroscience, Milan, Italy; IRCCS Humanitas Research Hospital, Lab of Circuits Neuroscience, Rozzano, Milan, ItalyEuropean Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy; Department of Physics and Astronomy, University of Florence, Sesto Fiorentino, Italy; National Institute of Optics - National Research Council (CNR-INO), Sesto Fiorentino, ItalyEuropean Laboratory for Non-linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy; Department of Physics and Astronomy, University of Florence, Sesto Fiorentino, Italy; National Institute of Optics - National Research Council (CNR-INO), Sesto Fiorentino, Italy; Corresponding authorSummary: Fear responses are functionally adaptive behaviors that are strengthened as memories. Indeed, detailed knowledge of the neural circuitry modulating fear memory could be the turning point for the comprehension of this emotion and its pathological states. A comprehensive understanding of the circuits mediating memory encoding, consolidation, and retrieval presents the fundamental technological challenge of analyzing activity in the entire brain with single-neuron resolution. In this context, we develop the brain-wide neuron quantification toolkit (BRANT) for mapping whole-brain neuronal activation at micron-scale resolution, combining tissue clearing, high-resolution light-sheet microscopy, and automated image analysis. The robustness and scalability of this method allow us to quantify the evolution of activity patterns across multiple phases of memory in mice. This approach highlights a strong sexual dimorphism in recruited circuits, which has no counterpart in the behavior. The methodology presented here paves the way for a comprehensive characterization of the evolution of fear memory.http://www.sciencedirect.com/science/article/pii/S2211124723009191CP: Neuroscience |
| spellingShingle | Alessandra Franceschini Giacomo Mazzamuto Curzio Checcucci Lorenzo Chicchi Duccio Fanelli Irene Costantini Maria Beatrice Passani Bianca Ambrogina Silva Francesco Saverio Pavone Ludovico Silvestri Brain-wide neuron quantification toolkit reveals strong sexual dimorphism in the evolution of fear memory Cell Reports CP: Neuroscience |
| title | Brain-wide neuron quantification toolkit reveals strong sexual dimorphism in the evolution of fear memory |
| title_full | Brain-wide neuron quantification toolkit reveals strong sexual dimorphism in the evolution of fear memory |
| title_fullStr | Brain-wide neuron quantification toolkit reveals strong sexual dimorphism in the evolution of fear memory |
| title_full_unstemmed | Brain-wide neuron quantification toolkit reveals strong sexual dimorphism in the evolution of fear memory |
| title_short | Brain-wide neuron quantification toolkit reveals strong sexual dimorphism in the evolution of fear memory |
| title_sort | brain wide neuron quantification toolkit reveals strong sexual dimorphism in the evolution of fear memory |
| topic | CP: Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723009191 |
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