Generation of iPSC lines derived from skin fibroblasts of two healthy controls using non-transmissible form of Sendai Virus
We established two iPSC lines starting from skin fibroblasts of two healthy individuals using Sendai-virus-based technique. The obtained iPSCs were characterized showing same STR profile as starting fibroblasts, normal karyotype, loss of stemness vectors, expression of stemness markers, both through...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506124000308 |
| _version_ | 1797311588952178688 |
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| author | A. Orsi V. Serpieri C. Mazzotta M. Cotta Ramusino E. Rossi S. Cerri E.M. Valente |
| author_facet | A. Orsi V. Serpieri C. Mazzotta M. Cotta Ramusino E. Rossi S. Cerri E.M. Valente |
| author_sort | A. Orsi |
| collection | DOAJ |
| description | We established two iPSC lines starting from skin fibroblasts of two healthy individuals using Sendai-virus-based technique. The obtained iPSCs were characterized showing same STR profile as starting fibroblasts, normal karyotype, loss of stemness vectors, expression of stemness markers, both through real-time PCR and immunofluorescence, (OCT4, SOX2, TRA-1–60, NANOG and SSEA4) and in vitro differentiation into three germ layers. |
| first_indexed | 2024-03-08T02:02:12Z |
| format | Article |
| id | doaj.art-7d5670243979418ca60588053f66e466 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-03-08T02:02:12Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-7d5670243979418ca60588053f66e4662024-02-14T05:15:04ZengElsevierStem Cell Research1873-50612024-04-0176103332Generation of iPSC lines derived from skin fibroblasts of two healthy controls using non-transmissible form of Sendai VirusA. Orsi0V. Serpieri1C. Mazzotta2M. Cotta Ramusino3E. Rossi4S. Cerri5E.M. Valente6Department of Molecular Medicine, University of Pavia, Pavia, ItalyDepartment of Molecular Medicine, University of Pavia, Pavia, ItalyDepartment of Molecular Medicine, University of Pavia, Pavia, ItalyUnit of Behavioral Neurology and Center for Cognitive Disorders and Dementia, IRCCS Mondino Foundation, Pavia, Italy; Dementia Research Center, IRCCS Mondino Foundation, Pavia, ItalyDepartment of Molecular Medicine, University of Pavia, Pavia, Italy; Neurogenetics Research Centre, IRCCS Mondino Foundation, Pavia, ItalyCellular and Molecular Neurobiology Unit, IRCCS Mondino Foundation, Pavia, ItalyDepartment of Molecular Medicine, University of Pavia, Pavia, Italy; Neurogenetics Research Centre, IRCCS Mondino Foundation, Pavia, Italy; Corresponding author.We established two iPSC lines starting from skin fibroblasts of two healthy individuals using Sendai-virus-based technique. The obtained iPSCs were characterized showing same STR profile as starting fibroblasts, normal karyotype, loss of stemness vectors, expression of stemness markers, both through real-time PCR and immunofluorescence, (OCT4, SOX2, TRA-1–60, NANOG and SSEA4) and in vitro differentiation into three germ layers.http://www.sciencedirect.com/science/article/pii/S1873506124000308 |
| spellingShingle | A. Orsi V. Serpieri C. Mazzotta M. Cotta Ramusino E. Rossi S. Cerri E.M. Valente Generation of iPSC lines derived from skin fibroblasts of two healthy controls using non-transmissible form of Sendai Virus Stem Cell Research |
| title | Generation of iPSC lines derived from skin fibroblasts of two healthy controls using non-transmissible form of Sendai Virus |
| title_full | Generation of iPSC lines derived from skin fibroblasts of two healthy controls using non-transmissible form of Sendai Virus |
| title_fullStr | Generation of iPSC lines derived from skin fibroblasts of two healthy controls using non-transmissible form of Sendai Virus |
| title_full_unstemmed | Generation of iPSC lines derived from skin fibroblasts of two healthy controls using non-transmissible form of Sendai Virus |
| title_short | Generation of iPSC lines derived from skin fibroblasts of two healthy controls using non-transmissible form of Sendai Virus |
| title_sort | generation of ipsc lines derived from skin fibroblasts of two healthy controls using non transmissible form of sendai virus |
| url | http://www.sciencedirect.com/science/article/pii/S1873506124000308 |
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