Whole genome sequence revealed the fine transmission map of carbapenem-resistant Klebsiella pneumonia isolates within a nosocomial outbreak
Abstract Background Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a major cause of nosocomial infections worldwide. The transmission route of CRKP isolates within an outbreak is rarely described. This study aimed to reveal the molecular characteristics and transmission route of CRKP isolates...
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| Format: | Article |
| Language: | English |
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BMC
2018-06-01
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| Series: | Antimicrobial Resistance and Infection Control |
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| Online Access: | http://link.springer.com/article/10.1186/s13756-018-0363-8 |
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| author | Wenjun Sui Haijian Zhou Pengcheng Du Lijun Wang Tian Qin Mei Wang Hongyu Ren Yanfei Huang Jing Hou Chen Chen Xinxin Lu |
| author_facet | Wenjun Sui Haijian Zhou Pengcheng Du Lijun Wang Tian Qin Mei Wang Hongyu Ren Yanfei Huang Jing Hou Chen Chen Xinxin Lu |
| author_sort | Wenjun Sui |
| collection | DOAJ |
| description | Abstract Background Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a major cause of nosocomial infections worldwide. The transmission route of CRKP isolates within an outbreak is rarely described. This study aimed to reveal the molecular characteristics and transmission route of CRKP isolates within an outbreak of nosocomial infection. Methods Collecting case information, active screening and targeted environmental monitoring were carried out. The antibiotic susceptibility, drug-resistant genes, molecular subtype and whole genome sequence of CRKP strains were analyzed. Results Between October and December 2011, 26 CRKP isolates were collected from eight patients in a surgical intensive care unit and subsequent transfer wards of Beijing Tongren hospital, China. All 26 isolates harbored bla KPC-2, bla SHV-1, and bla CTX-M-15 genes, had the same or similar pulsed-field gel electrophoresis patterns, and belonged to the sequence type 11 (ST11) clone. By comprehensive consideration of genomic and epidemiological information, a putative transmission map was constructed, including identifying one case as an independent event distinct from the other seven cases, and revealing two transmissions starting from the same case. Conclusions This study provided the first report confirming an outbreak caused by K. pneumoniae ST11 clone co-harboring the bla KPC-2, bla CTX-M-15, and bla SHV-1 genes, and suggested that comprehensive consideration of genomic and epidemiological data can yield a fine transmission map of an outbreak and facilitate the control of nosocomial transmission. |
| first_indexed | 2024-12-12T06:41:01Z |
| format | Article |
| id | doaj.art-7d581ef66ba1462faf6d7ec376b06406 |
| institution | Directory Open Access Journal |
| issn | 2047-2994 |
| language | English |
| last_indexed | 2024-12-12T06:41:01Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Antimicrobial Resistance and Infection Control |
| spelling | doaj.art-7d581ef66ba1462faf6d7ec376b064062022-12-22T00:34:20ZengBMCAntimicrobial Resistance and Infection Control2047-29942018-06-017111110.1186/s13756-018-0363-8Whole genome sequence revealed the fine transmission map of carbapenem-resistant Klebsiella pneumonia isolates within a nosocomial outbreakWenjun Sui0Haijian Zhou1Pengcheng Du2Lijun Wang3Tian Qin4Mei Wang5Hongyu Ren6Yanfei Huang7Jing Hou8Chen Chen9Xinxin Lu10Department of Clinical Laboratory, Beijing Tongren Hospital, Capital Medical UniversityState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionBeijing Key Laboratory of Emerging Infectious Diseases, Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical UniversityDepartment of Clinical Laboratory, Beijing Tongren Hospital, Capital Medical UniversityState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionDepartment of Clinical Laboratory, Beijing Tongren Hospital, Capital Medical UniversityState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionDepartment of Clinical Laboratory, Beijing Tongren Hospital, Capital Medical UniversitySurgical Intensive Care Unit, Beijing Tongren Hospital, Capital Medical UniversityBeijing Key Laboratory of Emerging Infectious Diseases, Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical UniversityDepartment of Clinical Laboratory, Beijing Tongren Hospital, Capital Medical UniversityAbstract Background Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a major cause of nosocomial infections worldwide. The transmission route of CRKP isolates within an outbreak is rarely described. This study aimed to reveal the molecular characteristics and transmission route of CRKP isolates within an outbreak of nosocomial infection. Methods Collecting case information, active screening and targeted environmental monitoring were carried out. The antibiotic susceptibility, drug-resistant genes, molecular subtype and whole genome sequence of CRKP strains were analyzed. Results Between October and December 2011, 26 CRKP isolates were collected from eight patients in a surgical intensive care unit and subsequent transfer wards of Beijing Tongren hospital, China. All 26 isolates harbored bla KPC-2, bla SHV-1, and bla CTX-M-15 genes, had the same or similar pulsed-field gel electrophoresis patterns, and belonged to the sequence type 11 (ST11) clone. By comprehensive consideration of genomic and epidemiological information, a putative transmission map was constructed, including identifying one case as an independent event distinct from the other seven cases, and revealing two transmissions starting from the same case. Conclusions This study provided the first report confirming an outbreak caused by K. pneumoniae ST11 clone co-harboring the bla KPC-2, bla CTX-M-15, and bla SHV-1 genes, and suggested that comprehensive consideration of genomic and epidemiological data can yield a fine transmission map of an outbreak and facilitate the control of nosocomial transmission.http://link.springer.com/article/10.1186/s13756-018-0363-8CarbapenemasesK. pneumoniaeKPC-2OutbreakWhole genome sequencing |
| spellingShingle | Wenjun Sui Haijian Zhou Pengcheng Du Lijun Wang Tian Qin Mei Wang Hongyu Ren Yanfei Huang Jing Hou Chen Chen Xinxin Lu Whole genome sequence revealed the fine transmission map of carbapenem-resistant Klebsiella pneumonia isolates within a nosocomial outbreak Antimicrobial Resistance and Infection Control Carbapenemases K. pneumoniae KPC-2 Outbreak Whole genome sequencing |
| title | Whole genome sequence revealed the fine transmission map of carbapenem-resistant Klebsiella pneumonia isolates within a nosocomial outbreak |
| title_full | Whole genome sequence revealed the fine transmission map of carbapenem-resistant Klebsiella pneumonia isolates within a nosocomial outbreak |
| title_fullStr | Whole genome sequence revealed the fine transmission map of carbapenem-resistant Klebsiella pneumonia isolates within a nosocomial outbreak |
| title_full_unstemmed | Whole genome sequence revealed the fine transmission map of carbapenem-resistant Klebsiella pneumonia isolates within a nosocomial outbreak |
| title_short | Whole genome sequence revealed the fine transmission map of carbapenem-resistant Klebsiella pneumonia isolates within a nosocomial outbreak |
| title_sort | whole genome sequence revealed the fine transmission map of carbapenem resistant klebsiella pneumonia isolates within a nosocomial outbreak |
| topic | Carbapenemases K. pneumoniae KPC-2 Outbreak Whole genome sequencing |
| url | http://link.springer.com/article/10.1186/s13756-018-0363-8 |
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