Fluorescence-based thermometry for precise estimation of nanoparticle laser-induced heating in cancerous cells at nanoscale
Photothermal therapy (PTT) has attracted increasing interest as a complementary method to be used alongside conventional therapies. Despite a great number of studies in this field, only a few have explored how temperatures affect the outcome of the PTT at nanoscale. In this work, we study the necros...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2022-08-01
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| Series: | Nanophotonics |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/nanoph-2022-0314 |
| _version_ | 1797947896934432768 |
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| author | Peltek Oleksii O. Ageev Eduard I. Talianov Pavel M. Mikushina Anna D. Epifanovskaya Olga S. Dubavik Aliaksei Veiko Vadim P. Lepik Kirill Zuev Dmitry A. Timin Alexander S. Zyuzin Mikhail V. |
| author_facet | Peltek Oleksii O. Ageev Eduard I. Talianov Pavel M. Mikushina Anna D. Epifanovskaya Olga S. Dubavik Aliaksei Veiko Vadim P. Lepik Kirill Zuev Dmitry A. Timin Alexander S. Zyuzin Mikhail V. |
| author_sort | Peltek Oleksii O. |
| collection | DOAJ |
| description | Photothermal therapy (PTT) has attracted increasing interest as a complementary method to be used alongside conventional therapies. Despite a great number of studies in this field, only a few have explored how temperatures affect the outcome of the PTT at nanoscale. In this work, we study the necrosis/apoptosis process of cancerous cells that occurs during PTT, using a combination of local laser heating and nanoscale fluorescence thermometry techniques. The temperature distribution within a whole cell was evaluated using fluorescence lifetime imaging microscopy during laser-induced hyperthermia. For this, gold nanorods were utilized as nanoheaters. The local near-infrared laser illumination produces a temperature gradient across the cells, which is precisely measured by nanoscale thermometry. This allows one to optimize the PTT conditions by varying concentration of gold nanorods associated with cells and laser power density. During the PTT procedure, such an approach enables an accurate determination of the percentages of apoptotic and necrotic cells using 2D and 3D models. According to the performed cell experiments, the influence of temperature increase during the PTT on cell death mechanisms has been verified and determined. Our investigations can improve the understanding of the PTT mechanisms and increase its therapeutic efficiency while avoiding any side effects. |
| first_indexed | 2024-04-10T21:33:47Z |
| format | Article |
| id | doaj.art-7d58ddf08c514858810e2e8aa50bfbc2 |
| institution | Directory Open Access Journal |
| issn | 2192-8614 |
| language | English |
| last_indexed | 2024-04-10T21:33:47Z |
| publishDate | 2022-08-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Nanophotonics |
| spelling | doaj.art-7d58ddf08c514858810e2e8aa50bfbc22023-01-19T12:47:01ZengDe GruyterNanophotonics2192-86142022-08-0111184323433510.1515/nanoph-2022-0314Fluorescence-based thermometry for precise estimation of nanoparticle laser-induced heating in cancerous cells at nanoscalePeltek Oleksii O.0Ageev Eduard I.1Talianov Pavel M.2Mikushina Anna D.3Epifanovskaya Olga S.4Dubavik Aliaksei5Veiko Vadim P.6Lepik Kirill7Zuev Dmitry A.8Timin Alexander S.9Zyuzin Mikhail V.10School of Physics and Engineering, ITMO University, Lomonosova 9, 191002, St. Petersburg, Russian FederationSchool of Physics and Engineering, ITMO University, Lomonosova 9, 191002, St. Petersburg, Russian FederationSchool of Physics and Engineering, ITMO University, Lomonosova 9, 191002, St. Petersburg, Russian FederationLaboratory of Renewable Energy Sources, Alferov University, Khlopina 8/3, 194021, St. Petersburg, Russian FederationRM Gorbacheva Research Institute of Pediatric Oncology, Hematology and Transplantation, Pavlov University, Lva Tolstogo 6/8, 191144, St. Petersburg, Russian FederationFaculty of Photonics, Center of Optical Information Technologies, ITMO University, Birzhevaya liniya 4, 199034, St. Petersburg, Russian FederationSchool of Physics and Engineering, ITMO University, Lomonosova 9, 191002, St. Petersburg, Russian FederationRM Gorbacheva Research Institute of Pediatric Oncology, Hematology and Transplantation, Pavlov University, Lva Tolstogo 6/8, 191144, St. Petersburg, Russian FederationSchool of Physics and Engineering, ITMO University, Lomonosova 9, 191002, St. Petersburg, Russian FederationSchool of Physics and Engineering, ITMO University, Lomonosova 9, 191002, St. Petersburg, Russian FederationSchool of Physics and Engineering, ITMO University, Lomonosova 9, 191002, St. Petersburg, Russian FederationPhotothermal therapy (PTT) has attracted increasing interest as a complementary method to be used alongside conventional therapies. Despite a great number of studies in this field, only a few have explored how temperatures affect the outcome of the PTT at nanoscale. In this work, we study the necrosis/apoptosis process of cancerous cells that occurs during PTT, using a combination of local laser heating and nanoscale fluorescence thermometry techniques. The temperature distribution within a whole cell was evaluated using fluorescence lifetime imaging microscopy during laser-induced hyperthermia. For this, gold nanorods were utilized as nanoheaters. The local near-infrared laser illumination produces a temperature gradient across the cells, which is precisely measured by nanoscale thermometry. This allows one to optimize the PTT conditions by varying concentration of gold nanorods associated with cells and laser power density. During the PTT procedure, such an approach enables an accurate determination of the percentages of apoptotic and necrotic cells using 2D and 3D models. According to the performed cell experiments, the influence of temperature increase during the PTT on cell death mechanisms has been verified and determined. Our investigations can improve the understanding of the PTT mechanisms and increase its therapeutic efficiency while avoiding any side effects.https://doi.org/10.1515/nanoph-2022-0314fluorescent nanothermometrylaser-induced heatingnecrosis/apoptosisphotodynamic therapy |
| spellingShingle | Peltek Oleksii O. Ageev Eduard I. Talianov Pavel M. Mikushina Anna D. Epifanovskaya Olga S. Dubavik Aliaksei Veiko Vadim P. Lepik Kirill Zuev Dmitry A. Timin Alexander S. Zyuzin Mikhail V. Fluorescence-based thermometry for precise estimation of nanoparticle laser-induced heating in cancerous cells at nanoscale Nanophotonics fluorescent nanothermometry laser-induced heating necrosis/apoptosis photodynamic therapy |
| title | Fluorescence-based thermometry for precise estimation of nanoparticle laser-induced heating in cancerous cells at nanoscale |
| title_full | Fluorescence-based thermometry for precise estimation of nanoparticle laser-induced heating in cancerous cells at nanoscale |
| title_fullStr | Fluorescence-based thermometry for precise estimation of nanoparticle laser-induced heating in cancerous cells at nanoscale |
| title_full_unstemmed | Fluorescence-based thermometry for precise estimation of nanoparticle laser-induced heating in cancerous cells at nanoscale |
| title_short | Fluorescence-based thermometry for precise estimation of nanoparticle laser-induced heating in cancerous cells at nanoscale |
| title_sort | fluorescence based thermometry for precise estimation of nanoparticle laser induced heating in cancerous cells at nanoscale |
| topic | fluorescent nanothermometry laser-induced heating necrosis/apoptosis photodynamic therapy |
| url | https://doi.org/10.1515/nanoph-2022-0314 |
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