EXOSC5 as a Novel Prognostic Marker Promotes Proliferation of Colorectal Cancer via Activating the ERK and AKT Pathways
Background and Objective: Exosome component 5 (EXOSC5) is a novel cancer-related gene that is aberrantly expressed in various malignances. However, the molecular mechanism and biological role of EXOSC5 have not been explored in colorectal cancer (CRC). In this study, we investigated the functions an...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-07-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2019.00643/full |
| _version_ | 1818265204669022208 |
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| author | Hongda Pan Hongda Pan Hongda Pan Hongda Pan Jingxin Pan Shibo Song Lei Ji Lei Ji Hong Lv Hong Lv Zhangru Yang Zhangru Yang |
| author_facet | Hongda Pan Hongda Pan Hongda Pan Hongda Pan Jingxin Pan Shibo Song Lei Ji Lei Ji Hong Lv Hong Lv Zhangru Yang Zhangru Yang |
| author_sort | Hongda Pan |
| collection | DOAJ |
| description | Background and Objective: Exosome component 5 (EXOSC5) is a novel cancer-related gene that is aberrantly expressed in various malignances. However, the molecular mechanism and biological role of EXOSC5 have not been explored in colorectal cancer (CRC). In this study, we investigated the functions and mechanisms by which EXOSC5 promotes the progression of CRC.Methods: EXOSC5 expressions in CRC cell lines and paired CRC and adjacent normal tissues were measured via quantitative real-time PCR (qRT-PCR), Western blot and immunohistochemistry (IHC). In vitro experiments including colony formation, Cell Counting Kit-8 (CCK-8), and flow cytometry and in vivo tumorigenesis assay were performed to explore the effects of EXOSC5 on growth of CRC. The impacts of EXOSC5 on ERK and Akt signaling pathways were measured by Western blot.Results: The mRNA and protein expression levels of EXOSC5 were up-regulated in CRC as compared to adjacent normal tissues. IHC analysis indicated that high EXOSC5 level was positively associated with poor prognosis. EXOSC5 overexpression facilitated the growth of CRC cells, while EXOSC5 knockdown led to decreased proliferation, G1/S phase transition arrest. The oncogenic functions of EXOSC5 were associated with activation of the ERK and Akt pathways in CRC.Conclusion: EXOSC5 is overexpressed in CRC and promotes CRC growth partly through activation of ERK and Akt signaling pathways. Accordingly, EXOSC5 may be a novel oncogene, and acts as a therapeutic target, or prognostic factor for CRC. |
| first_indexed | 2024-12-12T19:47:06Z |
| format | Article |
| id | doaj.art-7d5a446bc06548fab55f9ae1d5ccf583 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-12T19:47:06Z |
| publishDate | 2019-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-7d5a446bc06548fab55f9ae1d5ccf5832022-12-22T00:14:04ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2019-07-01910.3389/fonc.2019.00643465040EXOSC5 as a Novel Prognostic Marker Promotes Proliferation of Colorectal Cancer via Activating the ERK and AKT PathwaysHongda Pan0Hongda Pan1Hongda Pan2Hongda Pan3Jingxin Pan4Shibo Song5Lei Ji6Lei Ji7Hong Lv8Hong Lv9Zhangru Yang10Zhangru Yang11Department of Gastric Surgery, Fudan University Shanghai Cancer Center, Fudan University, Shanghai, ChinaDepartment of Hematology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, ChinaDepartment of Oncology, Shanghai Medical College, Fudan University, Shanghai, ChinaDepartment of Gastrointestinal Surgery, Beijing Hospital, Beijing, ChinaDepartment of Hematology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, ChinaDepartment of Gastrointestinal Surgery, Beijing Hospital, Beijing, ChinaDepartment of Gastric Surgery, Fudan University Shanghai Cancer Center, Fudan University, Shanghai, ChinaDepartment of Oncology, Shanghai Medical College, Fudan University, Shanghai, ChinaDepartment of Gastric Surgery, Fudan University Shanghai Cancer Center, Fudan University, Shanghai, ChinaDepartment of Oncology, Shanghai Medical College, Fudan University, Shanghai, ChinaDepartment of Gastric Surgery, Fudan University Shanghai Cancer Center, Fudan University, Shanghai, ChinaDepartment of Oncology, Shanghai Medical College, Fudan University, Shanghai, ChinaBackground and Objective: Exosome component 5 (EXOSC5) is a novel cancer-related gene that is aberrantly expressed in various malignances. However, the molecular mechanism and biological role of EXOSC5 have not been explored in colorectal cancer (CRC). In this study, we investigated the functions and mechanisms by which EXOSC5 promotes the progression of CRC.Methods: EXOSC5 expressions in CRC cell lines and paired CRC and adjacent normal tissues were measured via quantitative real-time PCR (qRT-PCR), Western blot and immunohistochemistry (IHC). In vitro experiments including colony formation, Cell Counting Kit-8 (CCK-8), and flow cytometry and in vivo tumorigenesis assay were performed to explore the effects of EXOSC5 on growth of CRC. The impacts of EXOSC5 on ERK and Akt signaling pathways were measured by Western blot.Results: The mRNA and protein expression levels of EXOSC5 were up-regulated in CRC as compared to adjacent normal tissues. IHC analysis indicated that high EXOSC5 level was positively associated with poor prognosis. EXOSC5 overexpression facilitated the growth of CRC cells, while EXOSC5 knockdown led to decreased proliferation, G1/S phase transition arrest. The oncogenic functions of EXOSC5 were associated with activation of the ERK and Akt pathways in CRC.Conclusion: EXOSC5 is overexpressed in CRC and promotes CRC growth partly through activation of ERK and Akt signaling pathways. Accordingly, EXOSC5 may be a novel oncogene, and acts as a therapeutic target, or prognostic factor for CRC.https://www.frontiersin.org/article/10.3389/fonc.2019.00643/fullEXOSC5proliferationcolorectal cancerAkt signaling pathwayERK signaling pathwayprognosis |
| spellingShingle | Hongda Pan Hongda Pan Hongda Pan Hongda Pan Jingxin Pan Shibo Song Lei Ji Lei Ji Hong Lv Hong Lv Zhangru Yang Zhangru Yang EXOSC5 as a Novel Prognostic Marker Promotes Proliferation of Colorectal Cancer via Activating the ERK and AKT Pathways Frontiers in Oncology EXOSC5 proliferation colorectal cancer Akt signaling pathway ERK signaling pathway prognosis |
| title | EXOSC5 as a Novel Prognostic Marker Promotes Proliferation of Colorectal Cancer via Activating the ERK and AKT Pathways |
| title_full | EXOSC5 as a Novel Prognostic Marker Promotes Proliferation of Colorectal Cancer via Activating the ERK and AKT Pathways |
| title_fullStr | EXOSC5 as a Novel Prognostic Marker Promotes Proliferation of Colorectal Cancer via Activating the ERK and AKT Pathways |
| title_full_unstemmed | EXOSC5 as a Novel Prognostic Marker Promotes Proliferation of Colorectal Cancer via Activating the ERK and AKT Pathways |
| title_short | EXOSC5 as a Novel Prognostic Marker Promotes Proliferation of Colorectal Cancer via Activating the ERK and AKT Pathways |
| title_sort | exosc5 as a novel prognostic marker promotes proliferation of colorectal cancer via activating the erk and akt pathways |
| topic | EXOSC5 proliferation colorectal cancer Akt signaling pathway ERK signaling pathway prognosis |
| url | https://www.frontiersin.org/article/10.3389/fonc.2019.00643/full |
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