Characterisation of Wild Strains of Lactic Acid Bacteria Isolated from Legumes and Their Biocontrol Potential against <i>Fusarium</i> spp.
Legumes are indispensable crops in sustainable agricultural systems because of their capability for biological nitrogen fixation owing to symbiosis with rhizobia and soil fertility restoration. Fungal pathogens from the genera <i>Fusarium</i> cause rotting and wilting and produce mycotox...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/13/12/2911 |
| _version_ | 1797382379861442560 |
|---|---|
| author | Miloslava Kavková Olga Bazalová Jaromír Cihlář Andrea Bohatá Jana Lencová Petr Konvalina |
| author_facet | Miloslava Kavková Olga Bazalová Jaromír Cihlář Andrea Bohatá Jana Lencová Petr Konvalina |
| author_sort | Miloslava Kavková |
| collection | DOAJ |
| description | Legumes are indispensable crops in sustainable agricultural systems because of their capability for biological nitrogen fixation owing to symbiosis with rhizobia and soil fertility restoration. Fungal pathogens from the genera <i>Fusarium</i> cause rotting and wilting and produce mycotoxins in plant tissues. The use of fungicides in sustainable agricultural systems is limited; therefore, the application of biological agents with antifungal activity against <i>Fusarium</i> spp. is desirable. Lactic acid bacteria (LAB) are promising control agents that produce a wide spectrum of functional metabolites. <i>Lactiplantibacillus plantarum</i> and other lactobacilli are the most intensively studied genera of LAB in relation to antifungal activity against <i>Fusarium</i> spp. However, LAB strains belonging to the lactobacilli and lactococci genera have not yet been isolated and characterised from legumes. Therefore, we aimed to obtain wild strains of LAB from legumes, screen them for functional characteristics with respect to their antifungal activity, and compare their antifungal activity against isolates of <i>Fusarium</i> spp. from legumes. Consequently, 31 LAB isolates belonging to 10 species were obtained and identified from legumes. Their functional properties, including genetics and proteomics, short-chain organic acid production, and antifungal activity against five <i>Fusarium</i> spp., of <i>Lactiplantibacillus plantarum</i>, <i>Lacticaseibacillus paracasei</i>, and <i>Lactiplantibacillus pentosus</i> isolates, were studied. Cell-free supernatants of <i>L. plantarum</i> and <i>L. pentosus</i> showed significant suppression of mycelial growth and conidial germination. |
| first_indexed | 2024-03-08T21:04:32Z |
| format | Article |
| id | doaj.art-7d5abaa4a519483e8382a66b48544033 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-08T21:04:32Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-7d5abaa4a519483e8382a66b485440332023-12-22T13:46:18ZengMDPI AGAgronomy2073-43952023-11-011312291110.3390/agronomy13122911Characterisation of Wild Strains of Lactic Acid Bacteria Isolated from Legumes and Their Biocontrol Potential against <i>Fusarium</i> spp.Miloslava Kavková0Olga Bazalová1Jaromír Cihlář2Andrea Bohatá3Jana Lencová4Petr Konvalina5Dairy Research Institute Ltd., Ke Dvoru 12a, 16000 Prague, Czech RepublicDairy Research Institute Ltd., Ke Dvoru 12a, 16000 Prague, Czech RepublicDairy Research Institute Ltd., Ke Dvoru 12a, 16000 Prague, Czech RepublicFaculty of Agriculture and Technology, University of South Bohemia in České Budějovice, Branišovská 1645/31A, 37005 České Budějovice, Czech RepublicFaculty of Agriculture and Technology, University of South Bohemia in České Budějovice, Branišovská 1645/31A, 37005 České Budějovice, Czech RepublicFaculty of Agriculture and Technology, University of South Bohemia in České Budějovice, Branišovská 1645/31A, 37005 České Budějovice, Czech RepublicLegumes are indispensable crops in sustainable agricultural systems because of their capability for biological nitrogen fixation owing to symbiosis with rhizobia and soil fertility restoration. Fungal pathogens from the genera <i>Fusarium</i> cause rotting and wilting and produce mycotoxins in plant tissues. The use of fungicides in sustainable agricultural systems is limited; therefore, the application of biological agents with antifungal activity against <i>Fusarium</i> spp. is desirable. Lactic acid bacteria (LAB) are promising control agents that produce a wide spectrum of functional metabolites. <i>Lactiplantibacillus plantarum</i> and other lactobacilli are the most intensively studied genera of LAB in relation to antifungal activity against <i>Fusarium</i> spp. However, LAB strains belonging to the lactobacilli and lactococci genera have not yet been isolated and characterised from legumes. Therefore, we aimed to obtain wild strains of LAB from legumes, screen them for functional characteristics with respect to their antifungal activity, and compare their antifungal activity against isolates of <i>Fusarium</i> spp. from legumes. Consequently, 31 LAB isolates belonging to 10 species were obtained and identified from legumes. Their functional properties, including genetics and proteomics, short-chain organic acid production, and antifungal activity against five <i>Fusarium</i> spp., of <i>Lactiplantibacillus plantarum</i>, <i>Lacticaseibacillus paracasei</i>, and <i>Lactiplantibacillus pentosus</i> isolates, were studied. Cell-free supernatants of <i>L. plantarum</i> and <i>L. pentosus</i> showed significant suppression of mycelial growth and conidial germination.https://www.mdpi.com/2073-4395/13/12/2911<i>Lactiplantibacillus plantarum</i>cell-free supernatantfunctional characteristicsantifungal activity<i>Fusarium</i> spp. |
| spellingShingle | Miloslava Kavková Olga Bazalová Jaromír Cihlář Andrea Bohatá Jana Lencová Petr Konvalina Characterisation of Wild Strains of Lactic Acid Bacteria Isolated from Legumes and Their Biocontrol Potential against <i>Fusarium</i> spp. Agronomy <i>Lactiplantibacillus plantarum</i> cell-free supernatant functional characteristics antifungal activity <i>Fusarium</i> spp. |
| title | Characterisation of Wild Strains of Lactic Acid Bacteria Isolated from Legumes and Their Biocontrol Potential against <i>Fusarium</i> spp. |
| title_full | Characterisation of Wild Strains of Lactic Acid Bacteria Isolated from Legumes and Their Biocontrol Potential against <i>Fusarium</i> spp. |
| title_fullStr | Characterisation of Wild Strains of Lactic Acid Bacteria Isolated from Legumes and Their Biocontrol Potential against <i>Fusarium</i> spp. |
| title_full_unstemmed | Characterisation of Wild Strains of Lactic Acid Bacteria Isolated from Legumes and Their Biocontrol Potential against <i>Fusarium</i> spp. |
| title_short | Characterisation of Wild Strains of Lactic Acid Bacteria Isolated from Legumes and Their Biocontrol Potential against <i>Fusarium</i> spp. |
| title_sort | characterisation of wild strains of lactic acid bacteria isolated from legumes and their biocontrol potential against i fusarium i spp |
| topic | <i>Lactiplantibacillus plantarum</i> cell-free supernatant functional characteristics antifungal activity <i>Fusarium</i> spp. |
| url | https://www.mdpi.com/2073-4395/13/12/2911 |
| work_keys_str_mv | AT miloslavakavkova characterisationofwildstrainsoflacticacidbacteriaisolatedfromlegumesandtheirbiocontrolpotentialagainstifusariumispp AT olgabazalova characterisationofwildstrainsoflacticacidbacteriaisolatedfromlegumesandtheirbiocontrolpotentialagainstifusariumispp AT jaromircihlar characterisationofwildstrainsoflacticacidbacteriaisolatedfromlegumesandtheirbiocontrolpotentialagainstifusariumispp AT andreabohata characterisationofwildstrainsoflacticacidbacteriaisolatedfromlegumesandtheirbiocontrolpotentialagainstifusariumispp AT janalencova characterisationofwildstrainsoflacticacidbacteriaisolatedfromlegumesandtheirbiocontrolpotentialagainstifusariumispp AT petrkonvalina characterisationofwildstrainsoflacticacidbacteriaisolatedfromlegumesandtheirbiocontrolpotentialagainstifusariumispp |