Low sensitivity of the COVID-19 antigen test (PANBIO™ COVID-19 Ag rapid test) to detect asymptomatic infections in health personnel of the National Institute of Respiratory Diseases
BackgroundCOVID-19 requires an early diagnosis to optimize management and limit transmission. SARS-CoV-2 is able to spread effectively. Infected asymptomatic individuals have been found to be contagious. RT-qPCR is the currently recommended laboratory method for diagnosing acute infection. However,...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2022.977924/full |
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| author | Eduardo Becerril Vargas Gabriel Cojuc-Konigsberg Mario Alberto Mujica Sánchez María Del Carmen García Colín Daniel Alfredo Camacho Corral Hugo Hansel Chávez Morales José Nicolas Aguirre Pineda Eduardo Martínez Bravo Alejandro Ortiz Martínez José Arturo Martínez Orozco Victor Manuel Rodríguez-Sánchez Jesús Ariel Mariscal Ochoa Brian Pantoja Jiménez Israel A. Morales Lozada Andrea Iraís Cuevas Rodriguez |
| author_facet | Eduardo Becerril Vargas Gabriel Cojuc-Konigsberg Mario Alberto Mujica Sánchez María Del Carmen García Colín Daniel Alfredo Camacho Corral Hugo Hansel Chávez Morales José Nicolas Aguirre Pineda Eduardo Martínez Bravo Alejandro Ortiz Martínez José Arturo Martínez Orozco Victor Manuel Rodríguez-Sánchez Jesús Ariel Mariscal Ochoa Brian Pantoja Jiménez Israel A. Morales Lozada Andrea Iraís Cuevas Rodriguez |
| author_sort | Eduardo Becerril Vargas |
| collection | DOAJ |
| description | BackgroundCOVID-19 requires an early diagnosis to optimize management and limit transmission. SARS-CoV-2 is able to spread effectively. Infected asymptomatic individuals have been found to be contagious. RT-qPCR is the currently recommended laboratory method for diagnosing acute infection. However, rapid antigen detection (RAD) tests are not only fast, but require less specialized training. The possibility of using RAD tests to identify asymptomatic patients is attractive, as it could effectively contribute to minimizing the hospital spread of SARS-CoV-2. The objective of the study was to determine the performance of RAD vs. RT-qPCR for the detection of asymptomatic cases in INER health personnel.MethodsIn order to follow WHO guidelines, generalized tests, a test station for health care workers was implemented on demand. A rapid test was carried out and a second sample was taken to be processed by RT-qPCR. With the results of both tests we conducted a retrospective study. Sensitivity, specificity, positive predictive value, negative predictive value and negative likelihood ratios were calculated.ResultsA total of 1640 RAD tests were performed in health care workers (mean age was 39, 69, 47% with a self-reported comorbidity). Participants provided 1,640 valid RAD/RT-qPCR test pairs with 2% testing positive via RT-qPCR. 12 RAD samples were positive for SARS-CoV-2. Overall sensitivity of the PANBIO ™ COVID-19 Ag Rapid Test test was 35.2%.ConclusionsRADs are not recommended for the detection of asymptomatic cases due to low performance. |
| first_indexed | 2024-12-10T03:35:04Z |
| format | Article |
| id | doaj.art-7d5b9825ba8848b88b369f1436e8f948 |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-12-10T03:35:04Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj.art-7d5b9825ba8848b88b369f1436e8f9482022-12-22T02:03:44ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2022-09-01910.3389/fmed.2022.977924977924Low sensitivity of the COVID-19 antigen test (PANBIO™ COVID-19 Ag rapid test) to detect asymptomatic infections in health personnel of the National Institute of Respiratory DiseasesEduardo Becerril VargasGabriel Cojuc-KonigsbergMario Alberto Mujica SánchezMaría Del Carmen García ColínDaniel Alfredo Camacho CorralHugo Hansel Chávez MoralesJosé Nicolas Aguirre PinedaEduardo Martínez BravoAlejandro Ortiz MartínezJosé Arturo Martínez OrozcoVictor Manuel Rodríguez-SánchezJesús Ariel Mariscal OchoaBrian Pantoja JiménezIsrael A. Morales LozadaAndrea Iraís Cuevas RodriguezBackgroundCOVID-19 requires an early diagnosis to optimize management and limit transmission. SARS-CoV-2 is able to spread effectively. Infected asymptomatic individuals have been found to be contagious. RT-qPCR is the currently recommended laboratory method for diagnosing acute infection. However, rapid antigen detection (RAD) tests are not only fast, but require less specialized training. The possibility of using RAD tests to identify asymptomatic patients is attractive, as it could effectively contribute to minimizing the hospital spread of SARS-CoV-2. The objective of the study was to determine the performance of RAD vs. RT-qPCR for the detection of asymptomatic cases in INER health personnel.MethodsIn order to follow WHO guidelines, generalized tests, a test station for health care workers was implemented on demand. A rapid test was carried out and a second sample was taken to be processed by RT-qPCR. With the results of both tests we conducted a retrospective study. Sensitivity, specificity, positive predictive value, negative predictive value and negative likelihood ratios were calculated.ResultsA total of 1640 RAD tests were performed in health care workers (mean age was 39, 69, 47% with a self-reported comorbidity). Participants provided 1,640 valid RAD/RT-qPCR test pairs with 2% testing positive via RT-qPCR. 12 RAD samples were positive for SARS-CoV-2. Overall sensitivity of the PANBIO ™ COVID-19 Ag Rapid Test test was 35.2%.ConclusionsRADs are not recommended for the detection of asymptomatic cases due to low performance.https://www.frontiersin.org/articles/10.3389/fmed.2022.977924/fullCOVID-19rapid antigen testsasymptomaticdetectionPCR |
| spellingShingle | Eduardo Becerril Vargas Gabriel Cojuc-Konigsberg Mario Alberto Mujica Sánchez María Del Carmen García Colín Daniel Alfredo Camacho Corral Hugo Hansel Chávez Morales José Nicolas Aguirre Pineda Eduardo Martínez Bravo Alejandro Ortiz Martínez José Arturo Martínez Orozco Victor Manuel Rodríguez-Sánchez Jesús Ariel Mariscal Ochoa Brian Pantoja Jiménez Israel A. Morales Lozada Andrea Iraís Cuevas Rodriguez Low sensitivity of the COVID-19 antigen test (PANBIO™ COVID-19 Ag rapid test) to detect asymptomatic infections in health personnel of the National Institute of Respiratory Diseases Frontiers in Medicine COVID-19 rapid antigen tests asymptomatic detection PCR |
| title | Low sensitivity of the COVID-19 antigen test (PANBIO™ COVID-19 Ag rapid test) to detect asymptomatic infections in health personnel of the National Institute of Respiratory Diseases |
| title_full | Low sensitivity of the COVID-19 antigen test (PANBIO™ COVID-19 Ag rapid test) to detect asymptomatic infections in health personnel of the National Institute of Respiratory Diseases |
| title_fullStr | Low sensitivity of the COVID-19 antigen test (PANBIO™ COVID-19 Ag rapid test) to detect asymptomatic infections in health personnel of the National Institute of Respiratory Diseases |
| title_full_unstemmed | Low sensitivity of the COVID-19 antigen test (PANBIO™ COVID-19 Ag rapid test) to detect asymptomatic infections in health personnel of the National Institute of Respiratory Diseases |
| title_short | Low sensitivity of the COVID-19 antigen test (PANBIO™ COVID-19 Ag rapid test) to detect asymptomatic infections in health personnel of the National Institute of Respiratory Diseases |
| title_sort | low sensitivity of the covid 19 antigen test panbio™ covid 19 ag rapid test to detect asymptomatic infections in health personnel of the national institute of respiratory diseases |
| topic | COVID-19 rapid antigen tests asymptomatic detection PCR |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2022.977924/full |
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