Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD)
Periapical odontogenic granulomas are among the most encountered pathology that involve the alveolar bone, with severe consequences such as bone resorption, the presence of inflammatory infiltrate and the formation of abnormal vascularization. The present study aimed to quantify the existence of the...
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MDPI AG
2023-10-01
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| Online Access: | https://www.mdpi.com/2227-9059/11/10/2709 |
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| author | Ciprian Roi Meda Lavinia Negruțiu Alexandra Roi Mircea Riviș Ruxandra Elena Luca Marius Raica Raluca Amalia Ceaușu Alexandru Cătălin Motofelea Pușa Nela Gaje |
| author_facet | Ciprian Roi Meda Lavinia Negruțiu Alexandra Roi Mircea Riviș Ruxandra Elena Luca Marius Raica Raluca Amalia Ceaușu Alexandru Cătălin Motofelea Pușa Nela Gaje |
| author_sort | Ciprian Roi |
| collection | DOAJ |
| description | Periapical odontogenic granulomas are among the most encountered pathology that involve the alveolar bone, with severe consequences such as bone resorption, the presence of inflammatory infiltrate and the formation of abnormal vascularization. The present study aimed to quantify the existence of the microvessel density (MVD), mast cell density (MCD) and heterogeneity of the encountered blood vessels. A total of 37 patients diagnosed with odontogenic periapical granulomas were included, and the gender distribution, age and localization of the pathological lesions was assessed. After the surgical removal of the periapical odontogenic granuloma, the collected tissue was fixed in 10% buffered formalin. Primary processing, morphological analysis and immunohistochemical staining was performed in order to characterize the altered tissue. The results outlined the presence of a high number of mast cells, especially in the area of the inflamed tissue; the high heterogeneity of the blood vessels; and increased MVD with positive CD34. The conclusions of the study focus on the key role of the mast cells and their implication in the initiation and development of the angiogenesis process, triggering the inflammatory response of the host. Nevertheless, periapical odontogenic granulomas develop as an inflammatory response to the interaction between the host’s immune system and microbial invasion. |
| first_indexed | 2024-03-10T21:25:32Z |
| format | Article |
| id | doaj.art-7d5c24951a1b437ba2392417503111b4 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T21:25:32Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-7d5c24951a1b437ba2392417503111b42023-11-19T15:45:58ZengMDPI AGBiomedicines2227-90592023-10-011110270910.3390/biomedicines11102709Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD)Ciprian Roi0Meda Lavinia Negruțiu1Alexandra Roi2Mircea Riviș3Ruxandra Elena Luca4Marius Raica5Raluca Amalia Ceaușu6Alexandru Cătălin Motofelea7Pușa Nela Gaje8Department of Anesthesiology and Oral Surgery, Multidisciplinary Center for Research, Evaluation, Diagnosis and Therapies in Oral Medicine, “Victor Babeș” University of Medicine and Pharmacy, Eftimie Murgu Sq. no. 2, 300041 Timisoara, RomaniaDepartment of Prostheses Technology and Dental Materials, Research Center in Dental Medicine Using Conventional and Alternative Technologies, Faculty of Dental Medicine, “Victor Babeș” University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timisoara, RomaniaDepartment of Oral Pathology, Multidisciplinary Center for Research, Evaluation, Diagnosis and Therapies in Oral Medicine, “Victor Babeș” University of Medicine and Pharmacy, Eftimie Murgu Sq. no. 2, 300041 Timisoara, RomaniaDepartment of Anesthesiology and Oral Surgery, Multidisciplinary Center for Research, Evaluation, Diagnosis and Therapies in Oral Medicine, “Victor Babeș” University of Medicine and Pharmacy, Eftimie Murgu Sq. no. 2, 300041 Timisoara, RomaniaDepartment of Oral Rehabilitation and Dental Emergencies, The Interdisciplinary Center for Dental Medical Research, Lasers and Innovative Technologies, “Victor Babeș” University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timisoara, RomaniaDepartment of Microscopic Morphology/Histology, Angiogenesis Research Center, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timisoara, RomaniaDepartment of Microscopic Morphology/Histology, Angiogenesis Research Center, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timisoara, RomaniaDepartment of Internal Medicine, Faculty of Medicine, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timisoara, RomaniaDepartment of Microscopic Morphology/Histology, Angiogenesis Research Center, “Victor Babeș” University of Medicine and Pharmacy, 300041 Timisoara, RomaniaPeriapical odontogenic granulomas are among the most encountered pathology that involve the alveolar bone, with severe consequences such as bone resorption, the presence of inflammatory infiltrate and the formation of abnormal vascularization. The present study aimed to quantify the existence of the microvessel density (MVD), mast cell density (MCD) and heterogeneity of the encountered blood vessels. A total of 37 patients diagnosed with odontogenic periapical granulomas were included, and the gender distribution, age and localization of the pathological lesions was assessed. After the surgical removal of the periapical odontogenic granuloma, the collected tissue was fixed in 10% buffered formalin. Primary processing, morphological analysis and immunohistochemical staining was performed in order to characterize the altered tissue. The results outlined the presence of a high number of mast cells, especially in the area of the inflamed tissue; the high heterogeneity of the blood vessels; and increased MVD with positive CD34. The conclusions of the study focus on the key role of the mast cells and their implication in the initiation and development of the angiogenesis process, triggering the inflammatory response of the host. Nevertheless, periapical odontogenic granulomas develop as an inflammatory response to the interaction between the host’s immune system and microbial invasion.https://www.mdpi.com/2227-9059/11/10/2709MVDmast cellsinflammationtooth infectionperiapical granulomaCD34 |
| spellingShingle | Ciprian Roi Meda Lavinia Negruțiu Alexandra Roi Mircea Riviș Ruxandra Elena Luca Marius Raica Raluca Amalia Ceaușu Alexandru Cătălin Motofelea Pușa Nela Gaje Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD) Biomedicines MVD mast cells inflammation tooth infection periapical granuloma CD34 |
| title | Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD) |
| title_full | Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD) |
| title_fullStr | Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD) |
| title_full_unstemmed | Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD) |
| title_short | Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD) |
| title_sort | human periapical odontogenic granulomas aspects of microvessel density mvd heterogeneity of blood vessels and mast cells density mcd |
| topic | MVD mast cells inflammation tooth infection periapical granuloma CD34 |
| url | https://www.mdpi.com/2227-9059/11/10/2709 |
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