Corneal Culture in Infectious Keratitis: Effect of the Inoculation Method and Media on the Corneal Culture Outcome
Background: To compare two different methods of corneal culture in infectious keratitis: multiple sampling for direct inoculation and enrichment (standard method) and a single sample via transport medium for indirect inoculation (indirect inoculation method). Methods: Prospective inclusion of patien...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/10/9/1810 |
| _version_ | 1797536859803353088 |
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| author | Susanna Sagerfors Chrysoula Karakoida Martin Sundqvist Birgitta Ejdervik Lindblad Bo Söderquist |
| author_facet | Susanna Sagerfors Chrysoula Karakoida Martin Sundqvist Birgitta Ejdervik Lindblad Bo Söderquist |
| author_sort | Susanna Sagerfors |
| collection | DOAJ |
| description | Background: To compare two different methods of corneal culture in infectious keratitis: multiple sampling for direct inoculation and enrichment (standard method) and a single sample via transport medium for indirect inoculation (indirect inoculation method). Methods: Prospective inclusion of patients fulfilling predefined criteria of infectious keratitis undergoing corneal culture according to both studied methods in a randomized order. Results: The standard method resulted in a significantly higher proportion of positive culture outcomes among the 94 included episodes of infectious keratitis (61%; 57/94) than the indirect inoculation method (44%; 41/94) (<i>p</i> = 0.002) and a significantly higher proportion of microorganisms than the indirect inoculation method, with a Cohen’s kappa of 0.38 (95% CI: 0.28–0.49) for agreement between the methods. Subanalysis of culture results showed that direct inoculation on gonococcal agar only combined with the indirect inoculation method resulted in a similar rate of culture positive patients and proportion of detected microorganisms to the standard method. Conclusion: Indirect inoculation of one corneal sample cannot replace direct inoculation of multiple corneal samples without loss of information. A combination of directly and indirectly inoculated samples can reduce the number of corneal samples by four without statistically significant differences in culture outcome or in the proportion of detected microorganisms. |
| first_indexed | 2024-03-10T12:06:48Z |
| format | Article |
| id | doaj.art-7d5f5ff95f5547b39bd27a5ea20b271b |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T12:06:48Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-7d5f5ff95f5547b39bd27a5ea20b271b2023-11-21T16:30:59ZengMDPI AGJournal of Clinical Medicine2077-03832021-04-01109181010.3390/jcm10091810Corneal Culture in Infectious Keratitis: Effect of the Inoculation Method and Media on the Corneal Culture OutcomeSusanna Sagerfors0Chrysoula Karakoida1Martin Sundqvist2Birgitta Ejdervik Lindblad3Bo Söderquist4Department of Ophthalmology, Faculty of Medicine and Health, Örebro University, SE 701 82 Örebro, SwedenDepartment of Laboratory Medicine, Clinical Microbiology, Faculty of Medicine and Health, Örebro University, SE 701 82 Örebro, SwedenDepartment of Laboratory Medicine, Clinical Microbiology, Faculty of Medicine and Health, Örebro University, SE 701 82 Örebro, SwedenDepartment of Ophthalmology, Faculty of Medicine and Health, Örebro University, SE 701 82 Örebro, SwedenDepartment of Laboratory Medicine, Clinical Microbiology, Faculty of Medicine and Health, Örebro University, SE 701 82 Örebro, SwedenBackground: To compare two different methods of corneal culture in infectious keratitis: multiple sampling for direct inoculation and enrichment (standard method) and a single sample via transport medium for indirect inoculation (indirect inoculation method). Methods: Prospective inclusion of patients fulfilling predefined criteria of infectious keratitis undergoing corneal culture according to both studied methods in a randomized order. Results: The standard method resulted in a significantly higher proportion of positive culture outcomes among the 94 included episodes of infectious keratitis (61%; 57/94) than the indirect inoculation method (44%; 41/94) (<i>p</i> = 0.002) and a significantly higher proportion of microorganisms than the indirect inoculation method, with a Cohen’s kappa of 0.38 (95% CI: 0.28–0.49) for agreement between the methods. Subanalysis of culture results showed that direct inoculation on gonococcal agar only combined with the indirect inoculation method resulted in a similar rate of culture positive patients and proportion of detected microorganisms to the standard method. Conclusion: Indirect inoculation of one corneal sample cannot replace direct inoculation of multiple corneal samples without loss of information. A combination of directly and indirectly inoculated samples can reduce the number of corneal samples by four without statistically significant differences in culture outcome or in the proportion of detected microorganisms.https://www.mdpi.com/2077-0383/10/9/1810infectious keratitiscorneal cultureindirect inoculationdirect inoculation |
| spellingShingle | Susanna Sagerfors Chrysoula Karakoida Martin Sundqvist Birgitta Ejdervik Lindblad Bo Söderquist Corneal Culture in Infectious Keratitis: Effect of the Inoculation Method and Media on the Corneal Culture Outcome Journal of Clinical Medicine infectious keratitis corneal culture indirect inoculation direct inoculation |
| title | Corneal Culture in Infectious Keratitis: Effect of the Inoculation Method and Media on the Corneal Culture Outcome |
| title_full | Corneal Culture in Infectious Keratitis: Effect of the Inoculation Method and Media on the Corneal Culture Outcome |
| title_fullStr | Corneal Culture in Infectious Keratitis: Effect of the Inoculation Method and Media on the Corneal Culture Outcome |
| title_full_unstemmed | Corneal Culture in Infectious Keratitis: Effect of the Inoculation Method and Media on the Corneal Culture Outcome |
| title_short | Corneal Culture in Infectious Keratitis: Effect of the Inoculation Method and Media on the Corneal Culture Outcome |
| title_sort | corneal culture in infectious keratitis effect of the inoculation method and media on the corneal culture outcome |
| topic | infectious keratitis corneal culture indirect inoculation direct inoculation |
| url | https://www.mdpi.com/2077-0383/10/9/1810 |
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