Preventive Effect of <i>Lactobacillus fermentum</i> CQPC03 on Activated Carbon-Induced Constipation in ICR Mice
<i>Background and objectives:</i> Paocai (pickled cabbage), which is fermented by lactic acid bacteria, is a traditional Chinese food. The microorganisms of Paocai were isolated and identified, and the constipation inhibition effect of one of the isolated <i>Lactobacillus</i>...
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MDPI AG
2018-11-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1010-660X/54/5/89 |
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| author | Jing Zhang Benshou Chen Baosi Liu Xianrong Zhou Jianfei Mu Qiang Wang Xin Zhao Zhennai Yang |
| author_facet | Jing Zhang Benshou Chen Baosi Liu Xianrong Zhou Jianfei Mu Qiang Wang Xin Zhao Zhennai Yang |
| author_sort | Jing Zhang |
| collection | DOAJ |
| description | <i>Background and objectives:</i> Paocai (pickled cabbage), which is fermented by lactic acid bacteria, is a traditional Chinese food. The microorganisms of Paocai were isolated and identified, and the constipation inhibition effect of one of the isolated <i>Lactobacillus</i> was investigated. <i>Materials and Methods:</i> The 16S rDNA technology was used for microbial identification. A mouse constipation model was established using activated carbon. After intragastric administration of <i>Lactobacillus</i> (10<sup>9</sup> CFU/mL), the mice were dissected to prepare pathological sections of the small intestine. Serum indicators were detected using kits, and the expression of small intestine-related mRNAs was detected by qPCR assay. <i>Results:</i> One strain of <i>Lactobacillus</i> was identified and named <i>Lactobacillus fermentum</i> CQPC03 (LF-CQPC03). Body weight and activated carbon propulsion rate were all higher in mice intragastrically administered with LF-CQPC03 compared with the control group, while the time to the first black stool in treated mice was lower than that in the control group. Serum assays showed that gastrin (Gas), endothelin (ET), and acetylcholinesterase (AchE) levels were significantly higher in the LF-CQPC03-treated mice than in the control group, while somatostatin (SS) levels were significantly lower than in the control mice. Mouse small intestine tissue showed that c-Kit, stem cell factor (SCF), and glial cell-derived neurotrophic factor (GDNF) mRNA expression levels were significantly higher in the LF-CQPC03 treated mice than in control mice, while transient receptor potential cation channel subfamily V member 1 (TRPV1) and inducible nitric oxide synthase (iNOS) expression levels were significantly lower in the LF-CQPC03 treated mice than in control mice. <i>Conclusions:</i> There is a better effect with high-dose LF-CQPC03, compared to the lower dose (LF-CQPC03-L), showing good probiotic potential, as well as development and application value. |
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| language | English |
| last_indexed | 2024-03-12T11:07:35Z |
| publishDate | 2018-11-01 |
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| spelling | doaj.art-7d5fd98bd8ee4987a3cb9d25eea6658b2023-09-02T03:30:59ZengMDPI AGMedicina1010-660X2018-11-015458910.3390/medicina54050089medicina54050089Preventive Effect of <i>Lactobacillus fermentum</i> CQPC03 on Activated Carbon-Induced Constipation in ICR MiceJing Zhang0Benshou Chen1Baosi Liu2Xianrong Zhou3Jianfei Mu4Qiang Wang5Xin Zhao6Zhennai Yang7Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU), Beijing 102488, ChinaEnvironmental and Quality Inspection College, Chongqing Chemical Industry Vocational College, Chongqing 401228, ChinaChongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, ChinaChongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, ChinaChongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, ChinaChongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU), Beijing 102488, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU), Beijing 102488, China<i>Background and objectives:</i> Paocai (pickled cabbage), which is fermented by lactic acid bacteria, is a traditional Chinese food. The microorganisms of Paocai were isolated and identified, and the constipation inhibition effect of one of the isolated <i>Lactobacillus</i> was investigated. <i>Materials and Methods:</i> The 16S rDNA technology was used for microbial identification. A mouse constipation model was established using activated carbon. After intragastric administration of <i>Lactobacillus</i> (10<sup>9</sup> CFU/mL), the mice were dissected to prepare pathological sections of the small intestine. Serum indicators were detected using kits, and the expression of small intestine-related mRNAs was detected by qPCR assay. <i>Results:</i> One strain of <i>Lactobacillus</i> was identified and named <i>Lactobacillus fermentum</i> CQPC03 (LF-CQPC03). Body weight and activated carbon propulsion rate were all higher in mice intragastrically administered with LF-CQPC03 compared with the control group, while the time to the first black stool in treated mice was lower than that in the control group. Serum assays showed that gastrin (Gas), endothelin (ET), and acetylcholinesterase (AchE) levels were significantly higher in the LF-CQPC03-treated mice than in the control group, while somatostatin (SS) levels were significantly lower than in the control mice. Mouse small intestine tissue showed that c-Kit, stem cell factor (SCF), and glial cell-derived neurotrophic factor (GDNF) mRNA expression levels were significantly higher in the LF-CQPC03 treated mice than in control mice, while transient receptor potential cation channel subfamily V member 1 (TRPV1) and inducible nitric oxide synthase (iNOS) expression levels were significantly lower in the LF-CQPC03 treated mice than in control mice. <i>Conclusions:</i> There is a better effect with high-dose LF-CQPC03, compared to the lower dose (LF-CQPC03-L), showing good probiotic potential, as well as development and application value.https://www.mdpi.com/1010-660X/54/5/89<i>Lactobacillus fermentum</i> CQPC03pickled cabbageconstipation16S rDNAmice |
| spellingShingle | Jing Zhang Benshou Chen Baosi Liu Xianrong Zhou Jianfei Mu Qiang Wang Xin Zhao Zhennai Yang Preventive Effect of <i>Lactobacillus fermentum</i> CQPC03 on Activated Carbon-Induced Constipation in ICR Mice Medicina <i>Lactobacillus fermentum</i> CQPC03 pickled cabbage constipation 16S rDNA mice |
| title | Preventive Effect of <i>Lactobacillus fermentum</i> CQPC03 on Activated Carbon-Induced Constipation in ICR Mice |
| title_full | Preventive Effect of <i>Lactobacillus fermentum</i> CQPC03 on Activated Carbon-Induced Constipation in ICR Mice |
| title_fullStr | Preventive Effect of <i>Lactobacillus fermentum</i> CQPC03 on Activated Carbon-Induced Constipation in ICR Mice |
| title_full_unstemmed | Preventive Effect of <i>Lactobacillus fermentum</i> CQPC03 on Activated Carbon-Induced Constipation in ICR Mice |
| title_short | Preventive Effect of <i>Lactobacillus fermentum</i> CQPC03 on Activated Carbon-Induced Constipation in ICR Mice |
| title_sort | preventive effect of i lactobacillus fermentum i cqpc03 on activated carbon induced constipation in icr mice |
| topic | <i>Lactobacillus fermentum</i> CQPC03 pickled cabbage constipation 16S rDNA mice |
| url | https://www.mdpi.com/1010-660X/54/5/89 |
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