High Carriage Rate of the Multiple Resistant Plasmids Harboring Quinolone Resistance Genes in <i>Enterobacter</i> spp. Isolated from Healthy Individuals
Antimicrobial-resistant bacteria causing intractable and even fatal infections are a major health concern. Resistant bacteria residing in the intestinal tract of healthy individuals present a silent threat because of frequent transmission via conjugation and transposition. Plasmids harboring quinolo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/1/15 |
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| author | Yongyan Long Xin Lu Xiansheng Ni Jiaqi Liu Mengyu Wang Xu Li Zhe Li Haijian Zhou Zhenpeng Li Kui Wu Wei Wang Liya Yang Jialiang Xu Haiying Chen Biao Kan |
| author_facet | Yongyan Long Xin Lu Xiansheng Ni Jiaqi Liu Mengyu Wang Xu Li Zhe Li Haijian Zhou Zhenpeng Li Kui Wu Wei Wang Liya Yang Jialiang Xu Haiying Chen Biao Kan |
| author_sort | Yongyan Long |
| collection | DOAJ |
| description | Antimicrobial-resistant bacteria causing intractable and even fatal infections are a major health concern. Resistant bacteria residing in the intestinal tract of healthy individuals present a silent threat because of frequent transmission via conjugation and transposition. Plasmids harboring quinolone resistance genes are increasingly detected in clinical isolates worldwide. Here, we investigated the molecular epidemiology of plasmid-mediated quinolone resistance (PMQR) in Gram-negative bacteria from healthy service trade workers. From 157 rectal swab samples, 125 ciprofloxacin-resistant strains, including 112 <i>Escherichia coli</i>, 10 <i>Klebsiella pneumoniae</i>, two <i>Proteus mirabilis</i>, and one <i>Citrobacter braakii</i>, were isolated. Multiplex PCR screening identified 39 strains harboring the PMQR genes (including 17 <i>qnr</i>,19 <i>aac(6</i><i>′)-Ib-cr</i>, and 22 <i>oqxA</i>/<i>oqxB</i>). The genome and plasmid sequences of 39 and 31 strains, respectively, were obtained by short- and long-read sequencing. PMQR genes mainly resided in the IncFIB, IncFII, and IncR plasmids, and coexisted with 3–11 other resistance genes. The high PMQR gene carriage rate among Gram-negative bacteria isolated from healthy individuals suggests the high-frequency transmission of these genes via plasmids, along with other resistance genes. Thus, healthy individuals may spread antibiotic-resistant bacterial, highlighting the need for improved monitoring and control of the spread of antibiotic-resistant bacteria and genes in healthy individuals. |
| first_indexed | 2024-03-10T03:02:48Z |
| format | Article |
| id | doaj.art-7d62b5c9d41a4a08b4eae354bb79231e |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T03:02:48Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-7d62b5c9d41a4a08b4eae354bb79231e2023-11-23T12:42:40ZengMDPI AGAntibiotics2079-63822021-12-011111510.3390/antibiotics11010015High Carriage Rate of the Multiple Resistant Plasmids Harboring Quinolone Resistance Genes in <i>Enterobacter</i> spp. Isolated from Healthy IndividualsYongyan Long0Xin Lu1Xiansheng Ni2Jiaqi Liu3Mengyu Wang4Xu Li5Zhe Li6Haijian Zhou7Zhenpeng Li8Kui Wu9Wei Wang10Liya Yang11Jialiang Xu12Haiying Chen13Biao Kan14The Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control, Jiangxi Province Key Laboratory of Animal-Origin and Vector-Borne Disease, Nanchang Center for Disease Control and Prevention, Nanchang 330038, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaThe Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control, Jiangxi Province Key Laboratory of Animal-Origin and Vector-Borne Disease, Nanchang Center for Disease Control and Prevention, Nanchang 330038, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaThe Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control, Jiangxi Province Key Laboratory of Animal-Origin and Vector-Borne Disease, Nanchang Center for Disease Control and Prevention, Nanchang 330038, ChinaBeijing Technology and Business University, Beijing 102206, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaThe Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control, Jiangxi Province Key Laboratory of Animal-Origin and Vector-Borne Disease, Nanchang Center for Disease Control and Prevention, Nanchang 330038, ChinaThe Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control, Jiangxi Province Key Laboratory of Animal-Origin and Vector-Borne Disease, Nanchang Center for Disease Control and Prevention, Nanchang 330038, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaBeijing Technology and Business University, Beijing 102206, ChinaThe Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control, Jiangxi Province Key Laboratory of Animal-Origin and Vector-Borne Disease, Nanchang Center for Disease Control and Prevention, Nanchang 330038, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, ChinaAntimicrobial-resistant bacteria causing intractable and even fatal infections are a major health concern. Resistant bacteria residing in the intestinal tract of healthy individuals present a silent threat because of frequent transmission via conjugation and transposition. Plasmids harboring quinolone resistance genes are increasingly detected in clinical isolates worldwide. Here, we investigated the molecular epidemiology of plasmid-mediated quinolone resistance (PMQR) in Gram-negative bacteria from healthy service trade workers. From 157 rectal swab samples, 125 ciprofloxacin-resistant strains, including 112 <i>Escherichia coli</i>, 10 <i>Klebsiella pneumoniae</i>, two <i>Proteus mirabilis</i>, and one <i>Citrobacter braakii</i>, were isolated. Multiplex PCR screening identified 39 strains harboring the PMQR genes (including 17 <i>qnr</i>,19 <i>aac(6</i><i>′)-Ib-cr</i>, and 22 <i>oqxA</i>/<i>oqxB</i>). The genome and plasmid sequences of 39 and 31 strains, respectively, were obtained by short- and long-read sequencing. PMQR genes mainly resided in the IncFIB, IncFII, and IncR plasmids, and coexisted with 3–11 other resistance genes. The high PMQR gene carriage rate among Gram-negative bacteria isolated from healthy individuals suggests the high-frequency transmission of these genes via plasmids, along with other resistance genes. Thus, healthy individuals may spread antibiotic-resistant bacterial, highlighting the need for improved monitoring and control of the spread of antibiotic-resistant bacteria and genes in healthy individuals.https://www.mdpi.com/2079-6382/11/1/15PMQR geneshealthy participantsciprofloxacin-resistant<i>Enterobacter</i> |
| spellingShingle | Yongyan Long Xin Lu Xiansheng Ni Jiaqi Liu Mengyu Wang Xu Li Zhe Li Haijian Zhou Zhenpeng Li Kui Wu Wei Wang Liya Yang Jialiang Xu Haiying Chen Biao Kan High Carriage Rate of the Multiple Resistant Plasmids Harboring Quinolone Resistance Genes in <i>Enterobacter</i> spp. Isolated from Healthy Individuals Antibiotics PMQR genes healthy participants ciprofloxacin-resistant <i>Enterobacter</i> |
| title | High Carriage Rate of the Multiple Resistant Plasmids Harboring Quinolone Resistance Genes in <i>Enterobacter</i> spp. Isolated from Healthy Individuals |
| title_full | High Carriage Rate of the Multiple Resistant Plasmids Harboring Quinolone Resistance Genes in <i>Enterobacter</i> spp. Isolated from Healthy Individuals |
| title_fullStr | High Carriage Rate of the Multiple Resistant Plasmids Harboring Quinolone Resistance Genes in <i>Enterobacter</i> spp. Isolated from Healthy Individuals |
| title_full_unstemmed | High Carriage Rate of the Multiple Resistant Plasmids Harboring Quinolone Resistance Genes in <i>Enterobacter</i> spp. Isolated from Healthy Individuals |
| title_short | High Carriage Rate of the Multiple Resistant Plasmids Harboring Quinolone Resistance Genes in <i>Enterobacter</i> spp. Isolated from Healthy Individuals |
| title_sort | high carriage rate of the multiple resistant plasmids harboring quinolone resistance genes in i enterobacter i spp isolated from healthy individuals |
| topic | PMQR genes healthy participants ciprofloxacin-resistant <i>Enterobacter</i> |
| url | https://www.mdpi.com/2079-6382/11/1/15 |
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