Gene expression patterns of novel visual and non-visual opsin families in immature and mature Japanese eel males
This study was carried out to identify and estimate physiological function of a new type of opsin subfamily present in the retina and whole brain tissues of Japanese eel using RNA–Seq transcriptome method. A total of 18 opsin subfamilies were identified through RNA–seq. The visual opsin family inclu...
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PeerJ Inc.
2020-02-01
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| Online Access: | https://peerj.com/articles/8326.pdf |
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| author | Jun-Hwan Byun Ji-Yeon Hyeon Eun-Su Kim Byeong-Hoon Kim Hiroshi Miyanishi Hirohiko Kagawa Yuki Takeuchi Se-Jae Kim Akihiro Takemura Sung-Pyo Hur |
| author_facet | Jun-Hwan Byun Ji-Yeon Hyeon Eun-Su Kim Byeong-Hoon Kim Hiroshi Miyanishi Hirohiko Kagawa Yuki Takeuchi Se-Jae Kim Akihiro Takemura Sung-Pyo Hur |
| author_sort | Jun-Hwan Byun |
| collection | DOAJ |
| description | This study was carried out to identify and estimate physiological function of a new type of opsin subfamily present in the retina and whole brain tissues of Japanese eel using RNA–Seq transcriptome method. A total of 18 opsin subfamilies were identified through RNA–seq. The visual opsin family included Rh2, SWS2, FWO, DSO, and Exo-Rhod. The non-visual opsin family included four types of melanopsin subfamily (Opn4x1, Opn4x2, Opn4m1, and Opn4m2), peropsin, two types of neuropsin subfamily (Opn5-like, Opn5), Opn3, three types of TMT opsin subfamily (TMT1, 2, 3), VA-opsin, and parapinopsin. In terms of changes in photoreceptor gene expression in the retina of sexually mature and immature male eels, DSO mRNA increased in the maturation group. Analysis of expression of opsin family gene in male eel brain before and after maturation revealed that DSO and SWS2 expression in terms of visual opsin mRNA increased in the sexually mature group. In terms of non-visual opsin mRNA, parapinopsin mRNA increased whereas that of TMT2 decreased in the fore-brain of the sexually mature group. The mRNA for parapinopsin increased in the mid-brain of the sexually mature group, whereas those of TMT1 and TMT3 increased in the hind-brain of the sexually mature group. DSO mRNA also increased in the retina after sexual maturation, and DSO and SWS2 mRNA increased in whole brain part, suggesting that DSO and SWS2 are closely related to sexual maturation. |
| first_indexed | 2024-03-09T07:06:22Z |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:06:22Z |
| publishDate | 2020-02-01 |
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| spelling | doaj.art-7d669b8c730442ae9b7947cd25cbeeea2023-12-03T09:30:31ZengPeerJ Inc.PeerJ2167-83592020-02-018e832610.7717/peerj.8326Gene expression patterns of novel visual and non-visual opsin families in immature and mature Japanese eel malesJun-Hwan Byun0Ji-Yeon Hyeon1Eun-Su Kim2Byeong-Hoon Kim3Hiroshi Miyanishi4Hirohiko Kagawa5Yuki Takeuchi6Se-Jae Kim7Akihiro Takemura8Sung-Pyo Hur9Graduate School of Engineering and Science, University of the Ryukyus, Nishihara, Okinawa, JapanJeju Research Institute, Korea Institute of Ocean Science & Technology, Jeju, Jeju, Republic of KoreaJeju Research Institute, Korea Institute of Ocean Science & Technology, Jeju, Jeju, Republic of KoreaMarine Science Institute, Jeju National University, Jeju, Jeju, Republic of KoreaDepartment of Marine Biology and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, Gakuen–Kibanadai–Nishi, Miyazaki, JapanDepartment of Marine Biology and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, Gakuen–Kibanadai–Nishi, Miyazaki, JapanDepartment of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Nishihara, Okinawa, JapanDepartment of Biology, Jeju National University, Jeju, Jeju, Republic of KoreaDepartment of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Nishihara, Okinawa, JapanJeju Research Institute, Korea Institute of Ocean Science & Technology, Jeju, Jeju, Republic of KoreaThis study was carried out to identify and estimate physiological function of a new type of opsin subfamily present in the retina and whole brain tissues of Japanese eel using RNA–Seq transcriptome method. A total of 18 opsin subfamilies were identified through RNA–seq. The visual opsin family included Rh2, SWS2, FWO, DSO, and Exo-Rhod. The non-visual opsin family included four types of melanopsin subfamily (Opn4x1, Opn4x2, Opn4m1, and Opn4m2), peropsin, two types of neuropsin subfamily (Opn5-like, Opn5), Opn3, three types of TMT opsin subfamily (TMT1, 2, 3), VA-opsin, and parapinopsin. In terms of changes in photoreceptor gene expression in the retina of sexually mature and immature male eels, DSO mRNA increased in the maturation group. Analysis of expression of opsin family gene in male eel brain before and after maturation revealed that DSO and SWS2 expression in terms of visual opsin mRNA increased in the sexually mature group. In terms of non-visual opsin mRNA, parapinopsin mRNA increased whereas that of TMT2 decreased in the fore-brain of the sexually mature group. The mRNA for parapinopsin increased in the mid-brain of the sexually mature group, whereas those of TMT1 and TMT3 increased in the hind-brain of the sexually mature group. DSO mRNA also increased in the retina after sexual maturation, and DSO and SWS2 mRNA increased in whole brain part, suggesting that DSO and SWS2 are closely related to sexual maturation.https://peerj.com/articles/8326.pdfPhotoreceptorJapanese eelAnguilla japonicaOpsinSex maturation |
| spellingShingle | Jun-Hwan Byun Ji-Yeon Hyeon Eun-Su Kim Byeong-Hoon Kim Hiroshi Miyanishi Hirohiko Kagawa Yuki Takeuchi Se-Jae Kim Akihiro Takemura Sung-Pyo Hur Gene expression patterns of novel visual and non-visual opsin families in immature and mature Japanese eel males PeerJ Photoreceptor Japanese eel Anguilla japonica Opsin Sex maturation |
| title | Gene expression patterns of novel visual and non-visual opsin families in immature and mature Japanese eel males |
| title_full | Gene expression patterns of novel visual and non-visual opsin families in immature and mature Japanese eel males |
| title_fullStr | Gene expression patterns of novel visual and non-visual opsin families in immature and mature Japanese eel males |
| title_full_unstemmed | Gene expression patterns of novel visual and non-visual opsin families in immature and mature Japanese eel males |
| title_short | Gene expression patterns of novel visual and non-visual opsin families in immature and mature Japanese eel males |
| title_sort | gene expression patterns of novel visual and non visual opsin families in immature and mature japanese eel males |
| topic | Photoreceptor Japanese eel Anguilla japonica Opsin Sex maturation |
| url | https://peerj.com/articles/8326.pdf |
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