Crl activates transcription by stabilizing active conformation of the master stress transcription initiation factor
σS is a master transcription initiation factor that protects bacterial cells from various harmful environmental stresses including antibiotic pressure. Although its mechanism remains unclear, it is known that full activation of σS-mediated transcription requires a σS-specific activator, Crl. In this...
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| Format: | Article |
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eLife Sciences Publications Ltd
2019-12-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/50928 |
| _version_ | 1811228128066928640 |
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| author | Juncao Xu Kaijie Cui Liqiang Shen Jing Shi Lingting Li Linlin You Chengli Fang Guoping Zhao Yu Feng Bei Yang Yu Zhang |
| author_facet | Juncao Xu Kaijie Cui Liqiang Shen Jing Shi Lingting Li Linlin You Chengli Fang Guoping Zhao Yu Feng Bei Yang Yu Zhang |
| author_sort | Juncao Xu |
| collection | DOAJ |
| description | σS is a master transcription initiation factor that protects bacterial cells from various harmful environmental stresses including antibiotic pressure. Although its mechanism remains unclear, it is known that full activation of σS-mediated transcription requires a σS-specific activator, Crl. In this study, we determined a 3.80 Å cryo-EM structure of an Escherichia coli transcription activation complex (E. coli Crl-TAC) comprising E. coli σS-RNA polymerase (σS-RNAP) holoenzyme, Crl, and a nucleic-acid scaffold. The structure reveals that Crl interacts with domain 2 of σS (σS2) and the RNAP core enzyme, but does not contact promoter DNA. Results from subsequent hydrogen-deuterium exchange mass spectrometry (HDX-MS) indicate that Crl stabilizes key structural motifs within σS2 to promote the assembly of the σS-RNAP holoenzyme and also to facilitate formation of an RNA polymerase–promoter DNA open complex (RPo). Our study demonstrates a unique DNA contact-independent mechanism of transcription activation, thereby defining a previously unrecognized mode of transcription activation in cells. |
| first_indexed | 2024-04-12T09:53:21Z |
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| id | doaj.art-7d66de756fe24a4bb02c35d7ddf80c6d |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T09:53:21Z |
| publishDate | 2019-12-01 |
| publisher | eLife Sciences Publications Ltd |
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| spelling | doaj.art-7d66de756fe24a4bb02c35d7ddf80c6d2022-12-22T03:37:47ZengeLife Sciences Publications LtdeLife2050-084X2019-12-01810.7554/eLife.50928Crl activates transcription by stabilizing active conformation of the master stress transcription initiation factorJuncao Xu0Kaijie Cui1Liqiang Shen2Jing Shi3Lingting Li4Linlin You5Chengli Fang6Guoping Zhao7Yu Feng8Bei Yang9https://orcid.org/0000-0001-5389-3859Yu Zhang10https://orcid.org/0000-0002-1778-8389Key Laboratory of Synthetic Biology,CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China; University of Chinese Academy of Sciences, Beijing, ChinaUniversity of Chinese Academy of Sciences, Beijing, China; Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, ChinaKey Laboratory of Synthetic Biology,CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China; University of Chinese Academy of Sciences, Beijing, ChinaDepartment of Biophysics, Zhejiang University School of Medicine, Hangzhou, China; Department of Pathology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaKey Laboratory of Synthetic Biology,CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China; University of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Synthetic Biology,CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China; University of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Synthetic Biology,CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China; University of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Synthetic Biology,CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China; Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai, China; Department of Microbiology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, China; State Key Laboratory of Genetic Engineering, Department of Microbiology, School of Life Sciences, Fudan University, Shanghai, China; Institute of Biomedical Sciences, Fudan University, Shanghai, ChinaDepartment of Biophysics, Zhejiang University School of Medicine, Hangzhou, China; Department of Pathology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, ChinaKey Laboratory of Synthetic Biology,CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, ChinaσS is a master transcription initiation factor that protects bacterial cells from various harmful environmental stresses including antibiotic pressure. Although its mechanism remains unclear, it is known that full activation of σS-mediated transcription requires a σS-specific activator, Crl. In this study, we determined a 3.80 Å cryo-EM structure of an Escherichia coli transcription activation complex (E. coli Crl-TAC) comprising E. coli σS-RNA polymerase (σS-RNAP) holoenzyme, Crl, and a nucleic-acid scaffold. The structure reveals that Crl interacts with domain 2 of σS (σS2) and the RNAP core enzyme, but does not contact promoter DNA. Results from subsequent hydrogen-deuterium exchange mass spectrometry (HDX-MS) indicate that Crl stabilizes key structural motifs within σS2 to promote the assembly of the σS-RNAP holoenzyme and also to facilitate formation of an RNA polymerase–promoter DNA open complex (RPo). Our study demonstrates a unique DNA contact-independent mechanism of transcription activation, thereby defining a previously unrecognized mode of transcription activation in cells.https://elifesciences.org/articles/50928transcription factorsRNA polymeraseCrltranscription activationsigma factorstress response |
| spellingShingle | Juncao Xu Kaijie Cui Liqiang Shen Jing Shi Lingting Li Linlin You Chengli Fang Guoping Zhao Yu Feng Bei Yang Yu Zhang Crl activates transcription by stabilizing active conformation of the master stress transcription initiation factor eLife transcription factors RNA polymerase Crl transcription activation sigma factor stress response |
| title | Crl activates transcription by stabilizing active conformation of the master stress transcription initiation factor |
| title_full | Crl activates transcription by stabilizing active conformation of the master stress transcription initiation factor |
| title_fullStr | Crl activates transcription by stabilizing active conformation of the master stress transcription initiation factor |
| title_full_unstemmed | Crl activates transcription by stabilizing active conformation of the master stress transcription initiation factor |
| title_short | Crl activates transcription by stabilizing active conformation of the master stress transcription initiation factor |
| title_sort | crl activates transcription by stabilizing active conformation of the master stress transcription initiation factor |
| topic | transcription factors RNA polymerase Crl transcription activation sigma factor stress response |
| url | https://elifesciences.org/articles/50928 |
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