Molecular characterization of avian influenza viruses (H5N2, H5N8, H5Nx and H9N2) isolated from chickens and ducks in the South of Egypt 2020 – 2021
This study was conducted to investigate the epidemiological situation of avian influenza viruses (AIV) and the molecular identification of the different AIV subtypes circulating among chickens and duck farms in South Egypt. A total of 143 samples were collected from chicken and duck farms in Qena (...
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| Format: | Article |
| Language: | English |
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Assiut University
2024-03-01
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| Series: | Journal of Advanced Veterinary Research |
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| Online Access: | https://www.advetresearch.com/index.php/AVR/article/view/1562 |
| _version_ | 1797267256385732608 |
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| author | Ahmed A.H. Ali Shimaa Mansour Sozun M. Hefeny Serageldeen Sultan |
| author_facet | Ahmed A.H. Ali Shimaa Mansour Sozun M. Hefeny Serageldeen Sultan |
| author_sort | Ahmed A.H. Ali |
| collection | DOAJ |
| description |
This study was conducted to investigate the epidemiological situation of avian influenza viruses (AIV) and the molecular identification of the different AIV subtypes circulating among chickens and duck farms in South Egypt. A total of 143 samples were collected from chicken and duck farms in Qena (n = 105) and Luxor (n=38) governorates during 2020. The organs and swabs were collected from diseased chickens and healthy ducks. The viruses were isolated in embryonated chicken eggs (ECEs) and their propagation was confirmed by hemagglutination test (AHT) and molecular detection of matrix gene by reverse transcription polymerase chain reaction (RT-PCR). AIV subtypes were identified by RT-PCR and specific primers. Phylogenetic analysis of sequenced partial H5, N2, and N8 genes was performed. The results revealed that 15 AIVs were subtyped to 2 H5N2, 2 H5N8, 8 H5Nx, and 2 H9N2. While an isolate could not be subtyped by used primers. The H5-based evolutionary tree of 4 isolates revealed their categorization with the 2.3.4.4b clade with close relation to H5N8 isolates from Egypt in 2021 and Kazakhstan in 2020. In conclusion, the occurrence of H5 and H9 viruses pays attention to a public health concern. Also, non-identified HxNx reveals a new AIV HA and NA subtype may be present among chickens.
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| first_indexed | 2024-03-07T14:02:08Z |
| format | Article |
| id | doaj.art-7d67b6d2c0164220a6c271928b38227a |
| institution | Directory Open Access Journal |
| issn | 2090-6269 2090-6277 |
| language | English |
| last_indexed | 2024-04-25T01:13:42Z |
| publishDate | 2024-03-01 |
| publisher | Assiut University |
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| series | Journal of Advanced Veterinary Research |
| spelling | doaj.art-7d67b6d2c0164220a6c271928b38227a2024-03-10T05:05:10ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772024-03-01143Molecular characterization of avian influenza viruses (H5N2, H5N8, H5Nx and H9N2) isolated from chickens and ducks in the South of Egypt 2020 – 2021Ahmed A.H. Ali0Shimaa Mansour1Sozun M. Hefeny2Serageldeen Sultan3Department of Virology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.Department of Virology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.Department of Virology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.Department of Microbiology, Virology Division, Faculty of South Valley University83523, Qena, Egypt. This study was conducted to investigate the epidemiological situation of avian influenza viruses (AIV) and the molecular identification of the different AIV subtypes circulating among chickens and duck farms in South Egypt. A total of 143 samples were collected from chicken and duck farms in Qena (n = 105) and Luxor (n=38) governorates during 2020. The organs and swabs were collected from diseased chickens and healthy ducks. The viruses were isolated in embryonated chicken eggs (ECEs) and their propagation was confirmed by hemagglutination test (AHT) and molecular detection of matrix gene by reverse transcription polymerase chain reaction (RT-PCR). AIV subtypes were identified by RT-PCR and specific primers. Phylogenetic analysis of sequenced partial H5, N2, and N8 genes was performed. The results revealed that 15 AIVs were subtyped to 2 H5N2, 2 H5N8, 8 H5Nx, and 2 H9N2. While an isolate could not be subtyped by used primers. The H5-based evolutionary tree of 4 isolates revealed their categorization with the 2.3.4.4b clade with close relation to H5N8 isolates from Egypt in 2021 and Kazakhstan in 2020. In conclusion, the occurrence of H5 and H9 viruses pays attention to a public health concern. Also, non-identified HxNx reveals a new AIV HA and NA subtype may be present among chickens. https://www.advetresearch.com/index.php/AVR/article/view/1562AIV H5 and H9 subtypes Ducks and chickens Phylogenetic analysis Egypt |
| spellingShingle | Ahmed A.H. Ali Shimaa Mansour Sozun M. Hefeny Serageldeen Sultan Molecular characterization of avian influenza viruses (H5N2, H5N8, H5Nx and H9N2) isolated from chickens and ducks in the South of Egypt 2020 – 2021 Journal of Advanced Veterinary Research AIV H5 and H9 subtypes Ducks and chickens Phylogenetic analysis Egypt |
| title | Molecular characterization of avian influenza viruses (H5N2, H5N8, H5Nx and H9N2) isolated from chickens and ducks in the South of Egypt 2020 – 2021 |
| title_full | Molecular characterization of avian influenza viruses (H5N2, H5N8, H5Nx and H9N2) isolated from chickens and ducks in the South of Egypt 2020 – 2021 |
| title_fullStr | Molecular characterization of avian influenza viruses (H5N2, H5N8, H5Nx and H9N2) isolated from chickens and ducks in the South of Egypt 2020 – 2021 |
| title_full_unstemmed | Molecular characterization of avian influenza viruses (H5N2, H5N8, H5Nx and H9N2) isolated from chickens and ducks in the South of Egypt 2020 – 2021 |
| title_short | Molecular characterization of avian influenza viruses (H5N2, H5N8, H5Nx and H9N2) isolated from chickens and ducks in the South of Egypt 2020 – 2021 |
| title_sort | molecular characterization of avian influenza viruses h5n2 h5n8 h5nx and h9n2 isolated from chickens and ducks in the south of egypt 2020 a€ 2021 |
| topic | AIV H5 and H9 subtypes Ducks and chickens Phylogenetic analysis Egypt |
| url | https://www.advetresearch.com/index.php/AVR/article/view/1562 |
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