GoPrime: Development of an In Silico Framework to Predict the Performance of Real-Time PCR Primers and Probes Using Foot-and-Mouth Disease Virus as a Model

GoPrime: Development of an In Silico Framework to Predict the Performance of Real-Time PCR Primers and Probes Using Foot-and-Mouth Disease Virus as a Model

Real-time PCR (rPCR) is a widely accepted diagnostic tool for the detection and quantification of nucleic acid targets. In order for these assays to achieve high sensitivity and specificity, primer and probe-template complementarity is essential; however, mismatches are often unavoidable and can res...

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Bibliographic Details
Main Authors: Emma L A Howson, Richard J Orton, Valerie Mioulet, Tiziana Lembo, Donald P King, Veronica L Fowler
Format: Article
Language:English
Published: MDPI AG 2020-04-01
Series:Pathogens
Subjects:
PCR
foot-and-mouth disease
diagnostics
in silico modeling framework
Online Access:https://www.mdpi.com/2076-0817/9/4/303

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