Dually Active Apigenin-Loaded Nanostructured Lipid Carriers for Cancer Treatment
Lorena Bonilla-Vidal,1,2 Marta Świtalska,3 Marta Espina,1,2 Joanna Wietrzyk,3 Maria Luisa García,1,2 Eliana B Souto,4,5 Anna Gliszczyńska,6,* Elena Sánchez López1,2,7,* 1Department of Pharmacy, Pharmaceutical Technology and Physical Chemistry, University of Barc...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-11-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/dually-active-apigenin-loaded-nanostructured-lipid-carriers-for-cancer-peer-reviewed-fulltext-article-IJN |
| _version_ | 1797486301447979008 |
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| author | Bonilla-Vidal L Świtalska M Espina M Wietrzyk J García ML Souto EB Gliszczyńska A Sánchez López E |
| author_facet | Bonilla-Vidal L Świtalska M Espina M Wietrzyk J García ML Souto EB Gliszczyńska A Sánchez López E |
| author_sort | Bonilla-Vidal L |
| collection | DOAJ |
| description | Lorena Bonilla-Vidal,1,2 Marta Świtalska,3 Marta Espina,1,2 Joanna Wietrzyk,3 Maria Luisa García,1,2 Eliana B Souto,4,5 Anna Gliszczyńska,6,* Elena Sánchez López1,2,7,* 1Department of Pharmacy, Pharmaceutical Technology and Physical Chemistry, University of Barcelona, Barcelona, Spain; 2Institute of Nanoscience and Nanotechnology (IN2UB), University of Barcelona, Barcelona, Spain; 3Department of Experimental Oncology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland; 4Laboratory of Pharmaceutical Technology, Department of Drug Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal; 5Associate Laboratory i4HB—Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, Porto, Portugal; 6Department of Food Chemistry and Biocatalysis, Wrocław University of Environmental and Life Sciences, Wrocław, Poland; 7Unit of Synthesis and Biomedical Applications of Peptides, IQAC-CSIC, Barcelona, Spain*These authors contributed equally to this workCorrespondence: Elena Sánchez López; Anna Gliszczyńska, Email esanchezlopez@ub.edu; anna.gliszczynska@upwr.edu.plPurpose: Cancer is one of the major causes of death worldwide affecting more than 19 million people. Traditional cancer therapies have many adverse effects and often result in unsatisfactory outcomes. Natural flavones, such as apigenin (APG), have demonstrated excellent antitumoral properties. However, they have a low aqueous solubility. To overcome this drawback, APG can be encapsulated in nanostructured lipid carriers (NLC). Therefore, we developed dual NLC encapsulating APG (APG-NLC) with a lipid matrix containing rosehip oil, which is known for its anti-inflammatory and antioxidant properties.Methods: Optimisation, physicochemical characterisation, biopharmaceutical behaviour, and therapeutic efficacy of this novel nanostructured system were assessed.Results: APG-NLC were optimized obtaining an average particle size below 200 nm, a surface charge of − 20 mV, and an encapsulation efficiency over 99%. The APG-NLC released APG in a sustained manner, and the results showed that the formulation was stable for more than 10 months. In vitro studies showed that APG-NLC possess significant antiangiogenic activity in ovo and selective antiproliferative activity in several cancer cell lines without exhibiting toxicity in healthy cells.Conclusion: APG-NLC containing rosehip oil were optimised. They exhibit suitable physicochemical parameters, storage stability for more than 10 months, and prolonged APG release. Moreover, APG-NLC were internalised inside tumour cells, showing the capacity to cause cytotoxicity in cancer cells without damaging healthy cells.Keywords: antitumoral, lipid nanoparticles, apigenin, rosehip oil |
| first_indexed | 2024-03-09T23:32:13Z |
| format | Article |
| id | doaj.art-7d6e0e1493264c47bc5820d406b3ceeb |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-03-09T23:32:13Z |
| publishDate | 2023-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-7d6e0e1493264c47bc5820d406b3ceeb2023-11-23T17:07:56ZengDove Medical PressInternational Journal of Nanomedicine1178-20132023-11-01Volume 186979699788442Dually Active Apigenin-Loaded Nanostructured Lipid Carriers for Cancer TreatmentBonilla-Vidal LŚwitalska MEspina MWietrzyk JGarcía MLSouto EBGliszczyńska ASánchez López ELorena Bonilla-Vidal,1,2 Marta Świtalska,3 Marta Espina,1,2 Joanna Wietrzyk,3 Maria Luisa García,1,2 Eliana B Souto,4,5 Anna Gliszczyńska,6,* Elena Sánchez López1,2,7,* 1Department of Pharmacy, Pharmaceutical Technology and Physical Chemistry, University of Barcelona, Barcelona, Spain; 2Institute of Nanoscience and Nanotechnology (IN2UB), University of Barcelona, Barcelona, Spain; 3Department of Experimental Oncology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland; 4Laboratory of Pharmaceutical Technology, Department of Drug Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal; 5Associate Laboratory i4HB—Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, Porto, Portugal; 6Department of Food Chemistry and Biocatalysis, Wrocław University of Environmental and Life Sciences, Wrocław, Poland; 7Unit of Synthesis and Biomedical Applications of Peptides, IQAC-CSIC, Barcelona, Spain*These authors contributed equally to this workCorrespondence: Elena Sánchez López; Anna Gliszczyńska, Email esanchezlopez@ub.edu; anna.gliszczynska@upwr.edu.plPurpose: Cancer is one of the major causes of death worldwide affecting more than 19 million people. Traditional cancer therapies have many adverse effects and often result in unsatisfactory outcomes. Natural flavones, such as apigenin (APG), have demonstrated excellent antitumoral properties. However, they have a low aqueous solubility. To overcome this drawback, APG can be encapsulated in nanostructured lipid carriers (NLC). Therefore, we developed dual NLC encapsulating APG (APG-NLC) with a lipid matrix containing rosehip oil, which is known for its anti-inflammatory and antioxidant properties.Methods: Optimisation, physicochemical characterisation, biopharmaceutical behaviour, and therapeutic efficacy of this novel nanostructured system were assessed.Results: APG-NLC were optimized obtaining an average particle size below 200 nm, a surface charge of − 20 mV, and an encapsulation efficiency over 99%. The APG-NLC released APG in a sustained manner, and the results showed that the formulation was stable for more than 10 months. In vitro studies showed that APG-NLC possess significant antiangiogenic activity in ovo and selective antiproliferative activity in several cancer cell lines without exhibiting toxicity in healthy cells.Conclusion: APG-NLC containing rosehip oil were optimised. They exhibit suitable physicochemical parameters, storage stability for more than 10 months, and prolonged APG release. Moreover, APG-NLC were internalised inside tumour cells, showing the capacity to cause cytotoxicity in cancer cells without damaging healthy cells.Keywords: antitumoral, lipid nanoparticles, apigenin, rosehip oilhttps://www.dovepress.com/dually-active-apigenin-loaded-nanostructured-lipid-carriers-for-cancer-peer-reviewed-fulltext-article-IJNantitumorallipid nanoparticlesapigeninrosehip oil. |
| spellingShingle | Bonilla-Vidal L Świtalska M Espina M Wietrzyk J García ML Souto EB Gliszczyńska A Sánchez López E Dually Active Apigenin-Loaded Nanostructured Lipid Carriers for Cancer Treatment International Journal of Nanomedicine antitumoral lipid nanoparticles apigenin rosehip oil. |
| title | Dually Active Apigenin-Loaded Nanostructured Lipid Carriers for Cancer Treatment |
| title_full | Dually Active Apigenin-Loaded Nanostructured Lipid Carriers for Cancer Treatment |
| title_fullStr | Dually Active Apigenin-Loaded Nanostructured Lipid Carriers for Cancer Treatment |
| title_full_unstemmed | Dually Active Apigenin-Loaded Nanostructured Lipid Carriers for Cancer Treatment |
| title_short | Dually Active Apigenin-Loaded Nanostructured Lipid Carriers for Cancer Treatment |
| title_sort | dually active apigenin loaded nanostructured lipid carriers for cancer treatment |
| topic | antitumoral lipid nanoparticles apigenin rosehip oil. |
| url | https://www.dovepress.com/dually-active-apigenin-loaded-nanostructured-lipid-carriers-for-cancer-peer-reviewed-fulltext-article-IJN |
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