Substrate adhesion regulates sealing zone architecture and dynamics in cultured osteoclasts.
The bone-degrading activity of osteoclasts depends on the formation of a cytoskeletal-adhesive super-structure known as the sealing zone (SZ). The SZ is a dynamic structure, consisting of a condensed array of podosomes, the elementary adhesion-mediating structures of osteoclasts, interconnected by F...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3230604?pdf=render |
| _version_ | 1818230386030804992 |
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| author | Fabian Anderegg Dafna Geblinger Peter Horvath Mirren Charnley Marcus Textor Lia Addadi Benjamin Geiger |
| author_facet | Fabian Anderegg Dafna Geblinger Peter Horvath Mirren Charnley Marcus Textor Lia Addadi Benjamin Geiger |
| author_sort | Fabian Anderegg |
| collection | DOAJ |
| description | The bone-degrading activity of osteoclasts depends on the formation of a cytoskeletal-adhesive super-structure known as the sealing zone (SZ). The SZ is a dynamic structure, consisting of a condensed array of podosomes, the elementary adhesion-mediating structures of osteoclasts, interconnected by F-actin filaments. The molecular composition and structure of the SZ were extensively investigated, yet despite its major importance for bone formation and remodelling, the mechanisms underlying its assembly and dynamics are still poorly understood. Here we determine the relations between matrix adhesiveness and the formation, stability and expansion of the SZ. By growing differentiated osteoclasts on micro-patterned glass substrates, where adhesive areas are separated by non-adhesive PLL-g-PEG barriers, we show that SZ growth and fusion strictly depend on the continuity of substrate adhesiveness, at the micrometer scale. We present a possible model for the role of mechanical forces in SZ formation and reorganization, inspired by the current data. |
| first_indexed | 2024-12-12T10:33:40Z |
| format | Article |
| id | doaj.art-7d6ef39fd5894be8811f7cc7548e4567 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T10:33:40Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7d6ef39fd5894be8811f7cc7548e45672022-12-22T00:27:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01612e2858310.1371/journal.pone.0028583Substrate adhesion regulates sealing zone architecture and dynamics in cultured osteoclasts.Fabian AndereggDafna GeblingerPeter HorvathMirren CharnleyMarcus TextorLia AddadiBenjamin GeigerThe bone-degrading activity of osteoclasts depends on the formation of a cytoskeletal-adhesive super-structure known as the sealing zone (SZ). The SZ is a dynamic structure, consisting of a condensed array of podosomes, the elementary adhesion-mediating structures of osteoclasts, interconnected by F-actin filaments. The molecular composition and structure of the SZ were extensively investigated, yet despite its major importance for bone formation and remodelling, the mechanisms underlying its assembly and dynamics are still poorly understood. Here we determine the relations between matrix adhesiveness and the formation, stability and expansion of the SZ. By growing differentiated osteoclasts on micro-patterned glass substrates, where adhesive areas are separated by non-adhesive PLL-g-PEG barriers, we show that SZ growth and fusion strictly depend on the continuity of substrate adhesiveness, at the micrometer scale. We present a possible model for the role of mechanical forces in SZ formation and reorganization, inspired by the current data.http://europepmc.org/articles/PMC3230604?pdf=render |
| spellingShingle | Fabian Anderegg Dafna Geblinger Peter Horvath Mirren Charnley Marcus Textor Lia Addadi Benjamin Geiger Substrate adhesion regulates sealing zone architecture and dynamics in cultured osteoclasts. PLoS ONE |
| title | Substrate adhesion regulates sealing zone architecture and dynamics in cultured osteoclasts. |
| title_full | Substrate adhesion regulates sealing zone architecture and dynamics in cultured osteoclasts. |
| title_fullStr | Substrate adhesion regulates sealing zone architecture and dynamics in cultured osteoclasts. |
| title_full_unstemmed | Substrate adhesion regulates sealing zone architecture and dynamics in cultured osteoclasts. |
| title_short | Substrate adhesion regulates sealing zone architecture and dynamics in cultured osteoclasts. |
| title_sort | substrate adhesion regulates sealing zone architecture and dynamics in cultured osteoclasts |
| url | http://europepmc.org/articles/PMC3230604?pdf=render |
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