Selection-free endogenous tagging of cell lines by bicistronic co-expression of the surface antigen NGFR

Selection-free endogenous tagging of cell lines by bicistronic co-expression of the surface antigen NGFR

Endogenous protein tagging, in contrast to exogenous overexpression of tagged proteins, allows to characterize specific protein functions under defined physiological or pathophysiological conditions without the influence of non-physiological protein levels. The development of generic and homology-in...

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Bibliographic Details
Main Authors: Marcel Seibert, Nina Kurrle, Verena Stolp, Heike Nürnberger, Sandra Tzschentke, Leon Börner, Frank Wempe, Hubert Serve, Frank Schnütgen
Format: Article
Language:English
Published: Elsevier 2022-01-01
Series:MethodsX
Subjects:
Selection-free endogenous tagging of cell lines
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016122003065

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