Invalid freeze-dried platelet gel promotes wound healing
Wound healing is the curative process of tissue injury, composed of three phases: the inflammatory phase, proliferative phase, followed by the maturation cum remodeling phase. Various treatment options were previously depicted for wound healing, however a treatment that accelerates these phases woul...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-01-01
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| Series: | Saudi Pharmaceutical Journal |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016418301579 |
| _version_ | 1811198336611385344 |
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| author | Aporn Chuncharunee Saranatra Waikakul Adisak Wongkajornsilp Viroje Chongkolwatana Lancharat Chuncharunee Aunchalee Sirimontaporn Thanaporn Rungruang Gopinathan Pillai Sreekanth |
| author_facet | Aporn Chuncharunee Saranatra Waikakul Adisak Wongkajornsilp Viroje Chongkolwatana Lancharat Chuncharunee Aunchalee Sirimontaporn Thanaporn Rungruang Gopinathan Pillai Sreekanth |
| author_sort | Aporn Chuncharunee |
| collection | DOAJ |
| description | Wound healing is the curative process of tissue injury, composed of three phases: the inflammatory phase, proliferative phase, followed by the maturation cum remodeling phase. Various treatment options were previously depicted for wound healing, however a treatment that accelerates these phases would be highly valuable. Platelet aggregation at the bleeding vessels and release of various growth factors are the most promising factors that stimulates the wound healing progress. In the present study, we hypothesized that the freeze-dried platelet which were normally discarded from the blood banks due to invalidity, might be promising to accelerate the phases of wound healing. The invalid freeze-dried platelets were prepared to a gel form called invalid freeze-dried platelet gel (IF-PG), which was tested for its efficacy in a cutaneous punch wound model in rats. Mupirocin antibiotic gel was used as a bio-equivalent formulation. The wound healing phases and changes in the wound sites were determined by assessing the wound sizes, histopathological analysis, immunohistochemical staining. The re-epithelialization at the wound sites at different time intervals till the wound closure was also determined. Our results suggest the beneficial effects of IF-PG; in reducing the wound area and accelerating wound closure in the cutaneous punch wound in rats. Histopathology and immunostaining results support the improvements in the wound when treated with IF-PG, which were similar to that of mupirocin antibiotic gel. Our preliminary findings also warrant the competency of IF-PG in modulating the different phases of wound healing process. In conclusion, IF-PG might be a resourceful alternative for the wound care management, however further studies are required to validate its impact on various growth factors before proceeding to clinical studies. Keywords: IF-PG, Wound healing, Animal model, Re-epithelialization |
| first_indexed | 2024-04-12T01:29:44Z |
| format | Article |
| id | doaj.art-7d7bc2bdb21d42bd8172274af44a2812 |
| institution | Directory Open Access Journal |
| issn | 1319-0164 |
| language | English |
| last_indexed | 2024-04-12T01:29:44Z |
| publishDate | 2019-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj.art-7d7bc2bdb21d42bd8172274af44a28122022-12-22T03:53:32ZengElsevierSaudi Pharmaceutical Journal1319-01642019-01-012713340Invalid freeze-dried platelet gel promotes wound healingAporn Chuncharunee0Saranatra Waikakul1Adisak Wongkajornsilp2Viroje Chongkolwatana3Lancharat Chuncharunee4Aunchalee Sirimontaporn5Thanaporn Rungruang6Gopinathan Pillai Sreekanth7Department of Anatomy, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandDepartment of Orthopedic Surgery, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandDepartment of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandDepartment of Transfusion Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandDepartment of Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, ThailandDepartment of Anatomy, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandDepartment of Anatomy, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandSiriraj Center of Research Excellence for Molecular Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Corresponding author at: Siriraj Center of Research Excellence for Molecular Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Road, Bangkok Noi, Bangkok 10700, Thailand.Wound healing is the curative process of tissue injury, composed of three phases: the inflammatory phase, proliferative phase, followed by the maturation cum remodeling phase. Various treatment options were previously depicted for wound healing, however a treatment that accelerates these phases would be highly valuable. Platelet aggregation at the bleeding vessels and release of various growth factors are the most promising factors that stimulates the wound healing progress. In the present study, we hypothesized that the freeze-dried platelet which were normally discarded from the blood banks due to invalidity, might be promising to accelerate the phases of wound healing. The invalid freeze-dried platelets were prepared to a gel form called invalid freeze-dried platelet gel (IF-PG), which was tested for its efficacy in a cutaneous punch wound model in rats. Mupirocin antibiotic gel was used as a bio-equivalent formulation. The wound healing phases and changes in the wound sites were determined by assessing the wound sizes, histopathological analysis, immunohistochemical staining. The re-epithelialization at the wound sites at different time intervals till the wound closure was also determined. Our results suggest the beneficial effects of IF-PG; in reducing the wound area and accelerating wound closure in the cutaneous punch wound in rats. Histopathology and immunostaining results support the improvements in the wound when treated with IF-PG, which were similar to that of mupirocin antibiotic gel. Our preliminary findings also warrant the competency of IF-PG in modulating the different phases of wound healing process. In conclusion, IF-PG might be a resourceful alternative for the wound care management, however further studies are required to validate its impact on various growth factors before proceeding to clinical studies. Keywords: IF-PG, Wound healing, Animal model, Re-epithelializationhttp://www.sciencedirect.com/science/article/pii/S1319016418301579 |
| spellingShingle | Aporn Chuncharunee Saranatra Waikakul Adisak Wongkajornsilp Viroje Chongkolwatana Lancharat Chuncharunee Aunchalee Sirimontaporn Thanaporn Rungruang Gopinathan Pillai Sreekanth Invalid freeze-dried platelet gel promotes wound healing Saudi Pharmaceutical Journal |
| title | Invalid freeze-dried platelet gel promotes wound healing |
| title_full | Invalid freeze-dried platelet gel promotes wound healing |
| title_fullStr | Invalid freeze-dried platelet gel promotes wound healing |
| title_full_unstemmed | Invalid freeze-dried platelet gel promotes wound healing |
| title_short | Invalid freeze-dried platelet gel promotes wound healing |
| title_sort | invalid freeze dried platelet gel promotes wound healing |
| url | http://www.sciencedirect.com/science/article/pii/S1319016418301579 |
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