Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle Injury
Muscle injuries are common musculoskeletal problems, but the pharmaceutical agent for muscle repair and healing is insufficient. Traditional Chinese Medicine (TCM) frequently uses topical treatments to treat muscle injuries, although scientific evidence supporting their efficacy is scarce. In this s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/16/8/1144 |
| _version_ | 1797583491024551936 |
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| author | Wing-Sum Siu Hui Ma Wen Cheng Wai-Ting Shum Ping-Chung Leung |
| author_facet | Wing-Sum Siu Hui Ma Wen Cheng Wai-Ting Shum Ping-Chung Leung |
| author_sort | Wing-Sum Siu |
| collection | DOAJ |
| description | Muscle injuries are common musculoskeletal problems, but the pharmaceutical agent for muscle repair and healing is insufficient. Traditional Chinese Medicine (TCM) frequently uses topical treatments to treat muscle injuries, although scientific evidence supporting their efficacy is scarce. In this study, an in vitro assay was used to test the cytotoxicity of a topical TCM formula containing Carthami Flos, Dipsaci Radix, and Rhei Rhizoma (CDR). Then, a muscle contusion rat model was developed to investigate the in vivo effect and basic mechanisms underlying CDR on muscle regeneration. The in vitro assay illustrated that CDR was non-cytotoxic to immortalized rat myoblast culture and increased cell viability. Histological results demonstrated that the CDR treatment facilitated muscle repair by increasing the number of new muscle fibers and promoting muscle integrity. The CDR treatment also upregulated the expression of Pax7, MyoD and myogenin, as evidenced by an immunohistochemical study. A gene expression analysis indicated that the CDR treatment accelerated the regeneration and remodeling phases during muscle repair. This study demonstrated that topical CDR treatment was effective at facilitating muscle injury repair. |
| first_indexed | 2024-03-10T23:39:38Z |
| format | Article |
| id | doaj.art-7d7c8b1a64174c9883ff1b75f86c301d |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T23:39:38Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-7d7c8b1a64174c9883ff1b75f86c301d2023-11-19T02:34:38ZengMDPI AGPharmaceuticals1424-82472023-08-01168114410.3390/ph16081144Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle InjuryWing-Sum Siu0Hui Ma1Wen Cheng2Wai-Ting Shum3Ping-Chung Leung4Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, ChinaInstitute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, ChinaInstitute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, ChinaInstitute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, ChinaInstitute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, ChinaMuscle injuries are common musculoskeletal problems, but the pharmaceutical agent for muscle repair and healing is insufficient. Traditional Chinese Medicine (TCM) frequently uses topical treatments to treat muscle injuries, although scientific evidence supporting their efficacy is scarce. In this study, an in vitro assay was used to test the cytotoxicity of a topical TCM formula containing Carthami Flos, Dipsaci Radix, and Rhei Rhizoma (CDR). Then, a muscle contusion rat model was developed to investigate the in vivo effect and basic mechanisms underlying CDR on muscle regeneration. The in vitro assay illustrated that CDR was non-cytotoxic to immortalized rat myoblast culture and increased cell viability. Histological results demonstrated that the CDR treatment facilitated muscle repair by increasing the number of new muscle fibers and promoting muscle integrity. The CDR treatment also upregulated the expression of Pax7, MyoD and myogenin, as evidenced by an immunohistochemical study. A gene expression analysis indicated that the CDR treatment accelerated the regeneration and remodeling phases during muscle repair. This study demonstrated that topical CDR treatment was effective at facilitating muscle injury repair.https://www.mdpi.com/1424-8247/16/8/1144Chinese Medicinetopical treatmentmuscle injurymuscle regeneration |
| spellingShingle | Wing-Sum Siu Hui Ma Wen Cheng Wai-Ting Shum Ping-Chung Leung Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle Injury Pharmaceuticals Chinese Medicine topical treatment muscle injury muscle regeneration |
| title | Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle Injury |
| title_full | Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle Injury |
| title_fullStr | Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle Injury |
| title_full_unstemmed | Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle Injury |
| title_short | Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle Injury |
| title_sort | traditional chinese medicine for topical treatment of skeletal muscle injury |
| topic | Chinese Medicine topical treatment muscle injury muscle regeneration |
| url | https://www.mdpi.com/1424-8247/16/8/1144 |
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