Salinity pretreatment synergies heat shock toxicity in cyanobacterium Anabaena PCC7120
This study was undertaken to bridge the knowledge gap pertaining to cyanobacteria’s response to pretreatment. The result elucidates the synergistic effect of pretreatment toxicity in cyanobacterium Anabaena PCC7120 on morphological and biochemical attributes. Chemical (salt) and physical (heat) stre...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1061927/full |
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| author | Rupanshee Srivastava Tripti Kanda Sadhana Yadav Nidhi Singh Shivam Yadav Rajesh Prajapati Vigya Kesari Neelam Atri |
| author_facet | Rupanshee Srivastava Tripti Kanda Sadhana Yadav Nidhi Singh Shivam Yadav Rajesh Prajapati Vigya Kesari Neelam Atri |
| author_sort | Rupanshee Srivastava |
| collection | DOAJ |
| description | This study was undertaken to bridge the knowledge gap pertaining to cyanobacteria’s response to pretreatment. The result elucidates the synergistic effect of pretreatment toxicity in cyanobacterium Anabaena PCC7120 on morphological and biochemical attributes. Chemical (salt) and physical (heat) stress-pretreated cells exhibited significant and reproducible changes in terms of growth pattern, morphology, pigments, lipid peroxidation, and antioxidant activity. Salinity pretreatment showed more than a five-fold decrease in the phycocyanin content but a six-fold and five-fold increase in carotenoid, lipid peroxidation (MDA content), and antioxidant activity (SOD and CAT) at 1 h and on 3rd day of treatment, respectively, giving the impression of stress-induced free radicals that are scavenged by antioxidants when compared to heat shock pretreatment. Furthermore, quantitative analysis of transcript (qRT-PCR) for FeSOD and MnSOD displayed a 3.6- and 1.8-fold increase in salt-pretreated (S-H) samples. The upregulation of transcript corresponding to salt pretreatment suggests a toxic role of salinity in synergizing heat shock. However, heat pretreatment suggests a protective role in mitigating salt toxicity. It could be inferred that pretreatment enhances the deleterious effect. However, it further showed that salinity (chemical stress) augments the damaging effect of heat shock (physical stress) more profoundly than physical stress on chemical stress possibly by modulating redox balance via activation of antioxidant responses. Our study reveals that upon pretreatment of heat, the negative effect of salt can be mitigated in filamentous cyanobacteria, thus providing a foundation for improved cyanobacterial tolerance to salt stress. |
| first_indexed | 2024-04-10T09:38:44Z |
| format | Article |
| id | doaj.art-7d7d12bc16474623a2dfda8c85958ef8 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T09:38:44Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-7d7d12bc16474623a2dfda8c85958ef82023-02-17T14:13:33ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-02-011410.3389/fmicb.2023.10619271061927Salinity pretreatment synergies heat shock toxicity in cyanobacterium Anabaena PCC7120Rupanshee Srivastava0Tripti Kanda1Sadhana Yadav2Nidhi Singh3Shivam Yadav4Rajesh Prajapati5Vigya Kesari6Neelam Atri7Department of Botany, Institute of Sciences, Banaras Hindu University, Varanasi, IndiaDepartment of Botany, Institute of Sciences, Banaras Hindu University, Varanasi, IndiaDepartment of Botany, Institute of Sciences, Banaras Hindu University, Varanasi, IndiaDepartment of Botany, Institute of Sciences, Banaras Hindu University, Varanasi, IndiaDepartment of Botany, Thakur Prasad Singh (T.P.S.) College, Patna, Bihar, IndiaDepartment of Botany, Institute of Sciences, Banaras Hindu University, Varanasi, IndiaDepartment of Botany, Institute of Sciences, Banaras Hindu University, Varanasi, IndiaDepartment of Botany, Mahila Mahavidyalaya (M.M.V.), Banaras Hindu University, Varanasi, IndiaThis study was undertaken to bridge the knowledge gap pertaining to cyanobacteria’s response to pretreatment. The result elucidates the synergistic effect of pretreatment toxicity in cyanobacterium Anabaena PCC7120 on morphological and biochemical attributes. Chemical (salt) and physical (heat) stress-pretreated cells exhibited significant and reproducible changes in terms of growth pattern, morphology, pigments, lipid peroxidation, and antioxidant activity. Salinity pretreatment showed more than a five-fold decrease in the phycocyanin content but a six-fold and five-fold increase in carotenoid, lipid peroxidation (MDA content), and antioxidant activity (SOD and CAT) at 1 h and on 3rd day of treatment, respectively, giving the impression of stress-induced free radicals that are scavenged by antioxidants when compared to heat shock pretreatment. Furthermore, quantitative analysis of transcript (qRT-PCR) for FeSOD and MnSOD displayed a 3.6- and 1.8-fold increase in salt-pretreated (S-H) samples. The upregulation of transcript corresponding to salt pretreatment suggests a toxic role of salinity in synergizing heat shock. However, heat pretreatment suggests a protective role in mitigating salt toxicity. It could be inferred that pretreatment enhances the deleterious effect. However, it further showed that salinity (chemical stress) augments the damaging effect of heat shock (physical stress) more profoundly than physical stress on chemical stress possibly by modulating redox balance via activation of antioxidant responses. Our study reveals that upon pretreatment of heat, the negative effect of salt can be mitigated in filamentous cyanobacteria, thus providing a foundation for improved cyanobacterial tolerance to salt stress.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1061927/fullcyanobacteriaAnabaena PCC7120salinityheat shockpretreatmentsstress |
| spellingShingle | Rupanshee Srivastava Tripti Kanda Sadhana Yadav Nidhi Singh Shivam Yadav Rajesh Prajapati Vigya Kesari Neelam Atri Salinity pretreatment synergies heat shock toxicity in cyanobacterium Anabaena PCC7120 Frontiers in Microbiology cyanobacteria Anabaena PCC7120 salinity heat shock pretreatments stress |
| title | Salinity pretreatment synergies heat shock toxicity in cyanobacterium Anabaena PCC7120 |
| title_full | Salinity pretreatment synergies heat shock toxicity in cyanobacterium Anabaena PCC7120 |
| title_fullStr | Salinity pretreatment synergies heat shock toxicity in cyanobacterium Anabaena PCC7120 |
| title_full_unstemmed | Salinity pretreatment synergies heat shock toxicity in cyanobacterium Anabaena PCC7120 |
| title_short | Salinity pretreatment synergies heat shock toxicity in cyanobacterium Anabaena PCC7120 |
| title_sort | salinity pretreatment synergies heat shock toxicity in cyanobacterium anabaena pcc7120 |
| topic | cyanobacteria Anabaena PCC7120 salinity heat shock pretreatments stress |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1061927/full |
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