A putative cation channel, NCA-1, and a novel protein, UNC-80, transmit neuronal activity in C. elegans.
Voltage-gated cation channels regulate neuronal excitability through selective ion flux. NALCN, a member of a protein family that is structurally related to the alpha1 subunits of voltage-gated sodium/calcium channels, was recently shown to regulate the resting membrane potentials by mediating sodiu...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2008-03-01
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| Series: | PLoS Biology |
| Online Access: | http://europepmc.org/articles/PMC2265767?pdf=render |
| _version_ | 1818651959898406912 |
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| author | Edward Yeh Sharon Ng Mi Zhang Magali Bouhours Ying Wang Min Wang Wesley Hung Kyota Aoyagi Katya Melnik-Martinez Michelle Li Fang Liu William R Schafer Mei Zhen |
| author_facet | Edward Yeh Sharon Ng Mi Zhang Magali Bouhours Ying Wang Min Wang Wesley Hung Kyota Aoyagi Katya Melnik-Martinez Michelle Li Fang Liu William R Schafer Mei Zhen |
| author_sort | Edward Yeh |
| collection | DOAJ |
| description | Voltage-gated cation channels regulate neuronal excitability through selective ion flux. NALCN, a member of a protein family that is structurally related to the alpha1 subunits of voltage-gated sodium/calcium channels, was recently shown to regulate the resting membrane potentials by mediating sodium leak and the firing of mouse neurons. We identified a role for the Caenorhabditis elegans NALCN homologues NCA-1 and NCA-2 in the propagation of neuronal activity from cell bodies to synapses. Loss of NCA activities leads to reduced synaptic transmission at neuromuscular junctions and frequent halting in locomotion. In vivo calcium imaging experiments further indicate that while calcium influx in the cell bodies of egg-laying motorneurons is unaffected by altered NCA activity, synaptic calcium transients are significantly reduced in nca loss-of-function mutants and increased in nca gain-of-function mutants. NCA-1 localizes along axons and is enriched at nonsynaptic regions. Its localization and function depend on UNC-79, and UNC-80, a novel conserved protein that is also enriched at nonsynaptic regions. We propose that NCA-1 and UNC-80 regulate neuronal activity at least in part by transmitting depolarization signals to synapses in C. elegans neurons. |
| first_indexed | 2024-12-17T02:14:24Z |
| format | Article |
| id | doaj.art-7d7e55d001644ab6b8b23d000a832887 |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-17T02:14:24Z |
| publishDate | 2008-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-7d7e55d001644ab6b8b23d000a8328872022-12-21T22:07:27ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852008-03-0163e5510.1371/journal.pbio.0060055A putative cation channel, NCA-1, and a novel protein, UNC-80, transmit neuronal activity in C. elegans.Edward YehSharon NgMi ZhangMagali BouhoursYing WangMin WangWesley HungKyota AoyagiKatya Melnik-MartinezMichelle LiFang LiuWilliam R SchaferMei ZhenVoltage-gated cation channels regulate neuronal excitability through selective ion flux. NALCN, a member of a protein family that is structurally related to the alpha1 subunits of voltage-gated sodium/calcium channels, was recently shown to regulate the resting membrane potentials by mediating sodium leak and the firing of mouse neurons. We identified a role for the Caenorhabditis elegans NALCN homologues NCA-1 and NCA-2 in the propagation of neuronal activity from cell bodies to synapses. Loss of NCA activities leads to reduced synaptic transmission at neuromuscular junctions and frequent halting in locomotion. In vivo calcium imaging experiments further indicate that while calcium influx in the cell bodies of egg-laying motorneurons is unaffected by altered NCA activity, synaptic calcium transients are significantly reduced in nca loss-of-function mutants and increased in nca gain-of-function mutants. NCA-1 localizes along axons and is enriched at nonsynaptic regions. Its localization and function depend on UNC-79, and UNC-80, a novel conserved protein that is also enriched at nonsynaptic regions. We propose that NCA-1 and UNC-80 regulate neuronal activity at least in part by transmitting depolarization signals to synapses in C. elegans neurons.http://europepmc.org/articles/PMC2265767?pdf=render |
| spellingShingle | Edward Yeh Sharon Ng Mi Zhang Magali Bouhours Ying Wang Min Wang Wesley Hung Kyota Aoyagi Katya Melnik-Martinez Michelle Li Fang Liu William R Schafer Mei Zhen A putative cation channel, NCA-1, and a novel protein, UNC-80, transmit neuronal activity in C. elegans. PLoS Biology |
| title | A putative cation channel, NCA-1, and a novel protein, UNC-80, transmit neuronal activity in C. elegans. |
| title_full | A putative cation channel, NCA-1, and a novel protein, UNC-80, transmit neuronal activity in C. elegans. |
| title_fullStr | A putative cation channel, NCA-1, and a novel protein, UNC-80, transmit neuronal activity in C. elegans. |
| title_full_unstemmed | A putative cation channel, NCA-1, and a novel protein, UNC-80, transmit neuronal activity in C. elegans. |
| title_short | A putative cation channel, NCA-1, and a novel protein, UNC-80, transmit neuronal activity in C. elegans. |
| title_sort | putative cation channel nca 1 and a novel protein unc 80 transmit neuronal activity in c elegans |
| url | http://europepmc.org/articles/PMC2265767?pdf=render |
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