Fine Mapping and Identification of a Candidate Gene for the Glossy Green Trait in Cabbage (<i>Brassica oleracea</i> var. <i>capitata</i>)
In higher plants, cuticular wax deposited on the surface of epidermal cells plays an important role in protecting the plant from biotic and abiotic stresses; however, the molecular mechanism of cuticular wax production is not completely understood. In this study, we identified a glossy green mutant...
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MDPI AG
2023-09-01
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| Series: | Plants |
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| author | Peiwen Wang Ziheng Li Lin Zhu Mozhen Cheng Xiuling Chen Aoxue Wang Chao Wang Xiaoxuan Zhang |
| author_facet | Peiwen Wang Ziheng Li Lin Zhu Mozhen Cheng Xiuling Chen Aoxue Wang Chao Wang Xiaoxuan Zhang |
| author_sort | Peiwen Wang |
| collection | DOAJ |
| description | In higher plants, cuticular wax deposited on the surface of epidermal cells plays an important role in protecting the plant from biotic and abiotic stresses; however, the molecular mechanism of cuticular wax production is not completely understood. In this study, we identified a glossy green mutant (98-1030gl) from the glaucous cabbage inbred line 98-1030. Scanning electron microscopy indicated that the amount of leaf cuticular wax significantly decreased in 98-1030gl. Genetic analysis showed that the glossy green trait was controlled by a single recessive gene. Bulked segregant analysis coupled with whole genome sequencing revealed that the candidate gene for the glossy green trait was located at 13,860,000–25,070,000 bp (11.21 Mb) on Chromosome 5. Based on the resequencing data of two parents and the F2 population, insertion-deletion markers were developed and used to reduce the candidate mapping region. The candidate gene (<i>Bol026949</i>) was then mapped in a 50.97 kb interval. Bol026949 belongs to the Agenet/Tudor domain protein family, whose members are predicted to be involved in chromatin remodeling and RNA transcription. Sequence analysis showed that a single nucleotide polymorphism mutation (C → G) in the second exon of <i>Bol026949</i> could result in the premature termination of its protein translation in 98-1030gl. Phylogenetic analysis showed that <i>Bol026949</i> is relatively conserved in cruciferous plants. Transcriptome profiling indicated that <i>Bol026949</i> might participate in cuticular wax production by regulating the transcript levels of genes involved in the post-translational cellular process and phytohormone signaling. Our findings provide an important clue for dissecting the regulatory mechanisms of cuticular wax production in cruciferous crops. |
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| publishDate | 2023-09-01 |
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| spelling | doaj.art-7d856a0664614324a7f077d59338f60c2023-11-19T12:33:29ZengMDPI AGPlants2223-77472023-09-011218334010.3390/plants12183340Fine Mapping and Identification of a Candidate Gene for the Glossy Green Trait in Cabbage (<i>Brassica oleracea</i> var. <i>capitata</i>)Peiwen Wang0Ziheng Li1Lin Zhu2Mozhen Cheng3Xiuling Chen4Aoxue Wang5Chao Wang6Xiaoxuan Zhang7Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin 150030, ChinaIn higher plants, cuticular wax deposited on the surface of epidermal cells plays an important role in protecting the plant from biotic and abiotic stresses; however, the molecular mechanism of cuticular wax production is not completely understood. In this study, we identified a glossy green mutant (98-1030gl) from the glaucous cabbage inbred line 98-1030. Scanning electron microscopy indicated that the amount of leaf cuticular wax significantly decreased in 98-1030gl. Genetic analysis showed that the glossy green trait was controlled by a single recessive gene. Bulked segregant analysis coupled with whole genome sequencing revealed that the candidate gene for the glossy green trait was located at 13,860,000–25,070,000 bp (11.21 Mb) on Chromosome 5. Based on the resequencing data of two parents and the F2 population, insertion-deletion markers were developed and used to reduce the candidate mapping region. The candidate gene (<i>Bol026949</i>) was then mapped in a 50.97 kb interval. Bol026949 belongs to the Agenet/Tudor domain protein family, whose members are predicted to be involved in chromatin remodeling and RNA transcription. Sequence analysis showed that a single nucleotide polymorphism mutation (C → G) in the second exon of <i>Bol026949</i> could result in the premature termination of its protein translation in 98-1030gl. Phylogenetic analysis showed that <i>Bol026949</i> is relatively conserved in cruciferous plants. Transcriptome profiling indicated that <i>Bol026949</i> might participate in cuticular wax production by regulating the transcript levels of genes involved in the post-translational cellular process and phytohormone signaling. Our findings provide an important clue for dissecting the regulatory mechanisms of cuticular wax production in cruciferous crops.https://www.mdpi.com/2223-7747/12/18/3340cuticular waxcabbagegenetic mappingBSA-seqAgenet/Tudor domain |
| spellingShingle | Peiwen Wang Ziheng Li Lin Zhu Mozhen Cheng Xiuling Chen Aoxue Wang Chao Wang Xiaoxuan Zhang Fine Mapping and Identification of a Candidate Gene for the Glossy Green Trait in Cabbage (<i>Brassica oleracea</i> var. <i>capitata</i>) Plants cuticular wax cabbage genetic mapping BSA-seq Agenet/Tudor domain |
| title | Fine Mapping and Identification of a Candidate Gene for the Glossy Green Trait in Cabbage (<i>Brassica oleracea</i> var. <i>capitata</i>) |
| title_full | Fine Mapping and Identification of a Candidate Gene for the Glossy Green Trait in Cabbage (<i>Brassica oleracea</i> var. <i>capitata</i>) |
| title_fullStr | Fine Mapping and Identification of a Candidate Gene for the Glossy Green Trait in Cabbage (<i>Brassica oleracea</i> var. <i>capitata</i>) |
| title_full_unstemmed | Fine Mapping and Identification of a Candidate Gene for the Glossy Green Trait in Cabbage (<i>Brassica oleracea</i> var. <i>capitata</i>) |
| title_short | Fine Mapping and Identification of a Candidate Gene for the Glossy Green Trait in Cabbage (<i>Brassica oleracea</i> var. <i>capitata</i>) |
| title_sort | fine mapping and identification of a candidate gene for the glossy green trait in cabbage i brassica oleracea i var i capitata i |
| topic | cuticular wax cabbage genetic mapping BSA-seq Agenet/Tudor domain |
| url | https://www.mdpi.com/2223-7747/12/18/3340 |
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