Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder
We have generated and characterized seven human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) from a single family, including unaffected and affected individuals clinically diagnosed with Autism Spectrum Disorder (ASD). The reprogramming of the PB...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-08-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506119301461 |
| _version_ | 1818143457331380224 |
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| author | Kiymet Bozaoglu Yujing Gao Edouard Stanley Miriam Fanjul-Fernández Natasha J. Brown Kate Pope Cherie C. Green Katerina Vlahos Koula Sourris Melanie Bahlo Martin Delatycki Ingrid Scheffer Paul J. Lockhart |
| author_facet | Kiymet Bozaoglu Yujing Gao Edouard Stanley Miriam Fanjul-Fernández Natasha J. Brown Kate Pope Cherie C. Green Katerina Vlahos Koula Sourris Melanie Bahlo Martin Delatycki Ingrid Scheffer Paul J. Lockhart |
| author_sort | Kiymet Bozaoglu |
| collection | DOAJ |
| description | We have generated and characterized seven human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) from a single family, including unaffected and affected individuals clinically diagnosed with Autism Spectrum Disorder (ASD). The reprogramming of the PBMCs was performed using non-integrative Sendai virus containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. All iPSC lines exhibited a normal karyotype and pluripotency was validated by immunofluorescence, flow cytometry and their ability to differentiate into the three embryonic germ layers. These iPSC lines are a valuable resource to study the molecular mechanisms underlying ASD. |
| first_indexed | 2024-12-11T11:31:58Z |
| format | Article |
| id | doaj.art-7d85cf9b4a764a35955099983a2154e6 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-11T11:31:58Z |
| publishDate | 2019-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-7d85cf9b4a764a35955099983a2154e62022-12-22T01:08:51ZengElsevierStem Cell Research1873-50612019-08-0139Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum DisorderKiymet Bozaoglu0Yujing Gao1Edouard Stanley2Miriam Fanjul-Fernández3Natasha J. Brown4Kate Pope5Cherie C. Green6Katerina Vlahos7Koula Sourris8Melanie Bahlo9Martin Delatycki10Ingrid Scheffer11Paul J. Lockhart12Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, AustraliaBruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, AustraliaDepartment of Paediatrics, University of Melbourne, Parkville, Australia; Murdoch Children's Research Institute, Parkville, Australia; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, AustraliaBruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia; Victorian Clinical Genetics Services, Victoria, AustraliaVictorian Clinical Genetics Services, Victoria, AustraliaBruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, AustraliaOlga Tennison Autism Research Centre, School of Psychology and Public Health, La Trobe University, Bundoora, Victoria, Australia; Department of Medicine, Austin Health, The University of Melbourne, Heidelberg, Victoria, AustraliaMurdoch Children's Research Institute, Parkville, AustraliaMurdoch Children's Research Institute, Parkville, AustraliaThe Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology, University of Melbourne, Melbourne, AustraliaBruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia; Victorian Clinical Genetics Services, Victoria, AustraliaDepartment of Paediatrics, University of Melbourne, Parkville, Australia; Department of Medicine, University of Melbourne, Austin Health, Melbourne, VIC, Australia; Florey Institute, Melbourne, VIC, Australia; Department of Neurology, Royal Children's Hospital, Melbourne, AustraliaBruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia; Corresponding author at: Murdoch Children's Research Institute, 50 Flemington Road, Parkville, Victoria 3052, Australia.We have generated and characterized seven human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) from a single family, including unaffected and affected individuals clinically diagnosed with Autism Spectrum Disorder (ASD). The reprogramming of the PBMCs was performed using non-integrative Sendai virus containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. All iPSC lines exhibited a normal karyotype and pluripotency was validated by immunofluorescence, flow cytometry and their ability to differentiate into the three embryonic germ layers. These iPSC lines are a valuable resource to study the molecular mechanisms underlying ASD.http://www.sciencedirect.com/science/article/pii/S1873506119301461 |
| spellingShingle | Kiymet Bozaoglu Yujing Gao Edouard Stanley Miriam Fanjul-Fernández Natasha J. Brown Kate Pope Cherie C. Green Katerina Vlahos Koula Sourris Melanie Bahlo Martin Delatycki Ingrid Scheffer Paul J. Lockhart Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder Stem Cell Research |
| title | Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder |
| title_full | Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder |
| title_fullStr | Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder |
| title_full_unstemmed | Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder |
| title_short | Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder |
| title_sort | generation of seven ipsc lines from peripheral blood mononuclear cells suitable to investigate autism spectrum disorder |
| url | http://www.sciencedirect.com/science/article/pii/S1873506119301461 |
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