MicroRNA-1270 Inhibits Cell Proliferation, Migration, and Invasion via Targeting IRF8 in Osteoblast-like Cell Lines
Osteoporosis (OP) is the most common bone disease affecting elderly individuals. The diagnosis of this pathology is most commonly made on the basis of bone fractures. Several microRNAs (miRNAs/miRs) have been identified as possible biomarkers for the diagnosis and treatment of OP. miRNAs can regulat...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/44/3/77 |
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| author | Eric Gustavo Ramírez-Salazar Erika Victoria Almeraya Tania Valentina López-Perez Zacarías Jiménez-Salas Nelly Patiño Rafael Velázquez-Cruz |
| author_facet | Eric Gustavo Ramírez-Salazar Erika Victoria Almeraya Tania Valentina López-Perez Zacarías Jiménez-Salas Nelly Patiño Rafael Velázquez-Cruz |
| author_sort | Eric Gustavo Ramírez-Salazar |
| collection | DOAJ |
| description | Osteoporosis (OP) is the most common bone disease affecting elderly individuals. The diagnosis of this pathology is most commonly made on the basis of bone fractures. Several microRNAs (miRNAs/miRs) have been identified as possible biomarkers for the diagnosis and treatment of OP. miRNAs can regulate gene expression, and determining their functions can provide potential pharmacological targets for treating OP. A previous study showed that miR-1270 was upregulated in monocytes derived from postmenopausal women with OP. Therefore, the present study aimed to uncover the role of miR-1270 in regulating bone metabolism. To reveal the mechanism underlying the regulatory effect of miR-1270 on interferon regulatory factor 8 (IRF8) expression, luciferase assay, reverse transcription-quantitative PCR, and Western blot analysis were performed. The results suggest that miR-1270 could regulate the mRNA and protein expression levels of IRF8 by directly binding to its 3′-untranslated region. The effects of miR-1270 overexpression and IRF8 silencing on cell proliferation, migration, and invasion were also evaluated. To the best of our knowledge, the current study was the first to support the crucial role of miR-1270 in bone metabolism via modulation of IRF8 expression. In addition, miR-1270 overexpression could attenuate human osteoblast-like cells’ proliferation and migration ability. |
| first_indexed | 2024-03-09T19:59:03Z |
| format | Article |
| id | doaj.art-7d86ac71577b40b9b77aadd37202d127 |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-09T19:59:03Z |
| publishDate | 2022-03-01 |
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| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-7d86ac71577b40b9b77aadd37202d1272023-11-24T00:48:22ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452022-03-014431182119010.3390/cimb44030077MicroRNA-1270 Inhibits Cell Proliferation, Migration, and Invasion via Targeting IRF8 in Osteoblast-like Cell LinesEric Gustavo Ramírez-Salazar0Erika Victoria Almeraya1Tania Valentina López-Perez2Zacarías Jiménez-Salas3Nelly Patiño4Rafael Velázquez-Cruz5Consejo Nacional de Ciencia y Tecnología (CONACYT)-Instituto Nacional de Medicina Genómica (INMEGEN), Mexico City 14610, MexicoLaboratorio de Genómica del Metabolismo Óseo, Instituto Nacional de Medicina Genómica (INMEGEN), Mexico City 14610, MexicoLaboratorio de Genómica del Metabolismo Óseo, Instituto Nacional de Medicina Genómica (INMEGEN), Mexico City 14610, MexicoCentro de Investigación en Nutrición y Salud Pública, Universidad Autónoma de Nuevo León, Monterrey 64460, MexicoUnidad de Citometría de Flujo (UCiF), Instituto Nacional de Medicina Genómica (INMEGEN), Mexico City 14610, MexicoLaboratorio de Genómica del Metabolismo Óseo, Instituto Nacional de Medicina Genómica (INMEGEN), Mexico City 14610, MexicoOsteoporosis (OP) is the most common bone disease affecting elderly individuals. The diagnosis of this pathology is most commonly made on the basis of bone fractures. Several microRNAs (miRNAs/miRs) have been identified as possible biomarkers for the diagnosis and treatment of OP. miRNAs can regulate gene expression, and determining their functions can provide potential pharmacological targets for treating OP. A previous study showed that miR-1270 was upregulated in monocytes derived from postmenopausal women with OP. Therefore, the present study aimed to uncover the role of miR-1270 in regulating bone metabolism. To reveal the mechanism underlying the regulatory effect of miR-1270 on interferon regulatory factor 8 (IRF8) expression, luciferase assay, reverse transcription-quantitative PCR, and Western blot analysis were performed. The results suggest that miR-1270 could regulate the mRNA and protein expression levels of IRF8 by directly binding to its 3′-untranslated region. The effects of miR-1270 overexpression and IRF8 silencing on cell proliferation, migration, and invasion were also evaluated. To the best of our knowledge, the current study was the first to support the crucial role of miR-1270 in bone metabolism via modulation of IRF8 expression. In addition, miR-1270 overexpression could attenuate human osteoblast-like cells’ proliferation and migration ability.https://www.mdpi.com/1467-3045/44/3/77bone metabolismgene regulationmicroRNAosteoblastosteoclastosteoporosis |
| spellingShingle | Eric Gustavo Ramírez-Salazar Erika Victoria Almeraya Tania Valentina López-Perez Zacarías Jiménez-Salas Nelly Patiño Rafael Velázquez-Cruz MicroRNA-1270 Inhibits Cell Proliferation, Migration, and Invasion via Targeting IRF8 in Osteoblast-like Cell Lines Current Issues in Molecular Biology bone metabolism gene regulation microRNA osteoblast osteoclast osteoporosis |
| title | MicroRNA-1270 Inhibits Cell Proliferation, Migration, and Invasion via Targeting IRF8 in Osteoblast-like Cell Lines |
| title_full | MicroRNA-1270 Inhibits Cell Proliferation, Migration, and Invasion via Targeting IRF8 in Osteoblast-like Cell Lines |
| title_fullStr | MicroRNA-1270 Inhibits Cell Proliferation, Migration, and Invasion via Targeting IRF8 in Osteoblast-like Cell Lines |
| title_full_unstemmed | MicroRNA-1270 Inhibits Cell Proliferation, Migration, and Invasion via Targeting IRF8 in Osteoblast-like Cell Lines |
| title_short | MicroRNA-1270 Inhibits Cell Proliferation, Migration, and Invasion via Targeting IRF8 in Osteoblast-like Cell Lines |
| title_sort | microrna 1270 inhibits cell proliferation migration and invasion via targeting irf8 in osteoblast like cell lines |
| topic | bone metabolism gene regulation microRNA osteoblast osteoclast osteoporosis |
| url | https://www.mdpi.com/1467-3045/44/3/77 |
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