Characterization of Nuclear Progesterone Receptor Isoforms in the Term Equine Placenta
In equine parturition, the role of progestins along with the nuclear progesterone receptor (nPR) signaling pathway in the placenta is not completely clarified. The progestins play an integral role in maintaining myometrial quiescence during the late stage of pregnancy via acting on nPR isoforms (PRA...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.660177/full |
| _version_ | 1818932956060712960 |
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| author | Ahmed M. Nagy Swanand R. Sathe Attia H. Atta Abdel Mohsen M. Hammam Walter H. Hsu |
| author_facet | Ahmed M. Nagy Swanand R. Sathe Attia H. Atta Abdel Mohsen M. Hammam Walter H. Hsu |
| author_sort | Ahmed M. Nagy |
| collection | DOAJ |
| description | In equine parturition, the role of progestins along with the nuclear progesterone receptor (nPR) signaling pathway in the placenta is not completely clarified. The progestins play an integral role in maintaining myometrial quiescence during the late stage of pregnancy via acting on nPR isoforms (PRA and PRB; PRB is more active than PRA). The current study aimed to determine the PRA and PRB expressions in the term equine placenta at the gene and protein levels. Six term equine placentas were used in this study. Reverse transcription polymerase chain reaction (RT-PCR) was used to quantify the mRNA expression for PRA and PRB. The protein expression was detected using the Western Blot technique. The results revealed that the mRNA and protein expressions for PRA were significantly higher (P < 0.0001) in the term equine placental tissue compared to the mRNA and protein expressions of PRB. These results demonstrated that nPRs are detectable in the term placenta of mares and PRA is the dominant isoform expressed. The present findings raised the possibility that the PRA plays an important role in the parturition process and expulsion of the placenta in mares. |
| first_indexed | 2024-12-20T04:40:43Z |
| format | Article |
| id | doaj.art-7d8da37d777c473e86bc81301e51c82b |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-20T04:40:43Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-7d8da37d777c473e86bc81301e51c82b2022-12-21T19:53:08ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-04-01810.3389/fvets.2021.660177660177Characterization of Nuclear Progesterone Receptor Isoforms in the Term Equine PlacentaAhmed M. Nagy0Swanand R. Sathe1Attia H. Atta2Abdel Mohsen M. Hammam3Walter H. Hsu4Department of Animal Reproduction and Artificial Insemination, Veterinary Research Division, National Research Center, Cairo, EgyptDepartment of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA, United StatesDepartment of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Cairo, EgyptDepartment of Animal Reproduction and Artificial Insemination, Veterinary Research Division, National Research Center, Cairo, EgyptDepartment of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA, United StatesIn equine parturition, the role of progestins along with the nuclear progesterone receptor (nPR) signaling pathway in the placenta is not completely clarified. The progestins play an integral role in maintaining myometrial quiescence during the late stage of pregnancy via acting on nPR isoforms (PRA and PRB; PRB is more active than PRA). The current study aimed to determine the PRA and PRB expressions in the term equine placenta at the gene and protein levels. Six term equine placentas were used in this study. Reverse transcription polymerase chain reaction (RT-PCR) was used to quantify the mRNA expression for PRA and PRB. The protein expression was detected using the Western Blot technique. The results revealed that the mRNA and protein expressions for PRA were significantly higher (P < 0.0001) in the term equine placental tissue compared to the mRNA and protein expressions of PRB. These results demonstrated that nPRs are detectable in the term placenta of mares and PRA is the dominant isoform expressed. The present findings raised the possibility that the PRA plays an important role in the parturition process and expulsion of the placenta in mares.https://www.frontiersin.org/articles/10.3389/fvets.2021.660177/fullplacentaequineprogesteronereceptorsPRAPRB |
| spellingShingle | Ahmed M. Nagy Swanand R. Sathe Attia H. Atta Abdel Mohsen M. Hammam Walter H. Hsu Characterization of Nuclear Progesterone Receptor Isoforms in the Term Equine Placenta Frontiers in Veterinary Science placenta equine progesterone receptors PRA PRB |
| title | Characterization of Nuclear Progesterone Receptor Isoforms in the Term Equine Placenta |
| title_full | Characterization of Nuclear Progesterone Receptor Isoforms in the Term Equine Placenta |
| title_fullStr | Characterization of Nuclear Progesterone Receptor Isoforms in the Term Equine Placenta |
| title_full_unstemmed | Characterization of Nuclear Progesterone Receptor Isoforms in the Term Equine Placenta |
| title_short | Characterization of Nuclear Progesterone Receptor Isoforms in the Term Equine Placenta |
| title_sort | characterization of nuclear progesterone receptor isoforms in the term equine placenta |
| topic | placenta equine progesterone receptors PRA PRB |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2021.660177/full |
| work_keys_str_mv | AT ahmedmnagy characterizationofnuclearprogesteronereceptorisoformsinthetermequineplacenta AT swanandrsathe characterizationofnuclearprogesteronereceptorisoformsinthetermequineplacenta AT attiahatta characterizationofnuclearprogesteronereceptorisoformsinthetermequineplacenta AT abdelmohsenmhammam characterizationofnuclearprogesteronereceptorisoformsinthetermequineplacenta AT walterhhsu characterizationofnuclearprogesteronereceptorisoformsinthetermequineplacenta |