Detection of antibodies against Leishmania species using enzyme-linked immunosorbent assay in cats from the western border of Brazil
ABSTRACT: Feline leishmaniosis is infrequent worldwide, and cats have been suggested as secondary reservoirs for the parasite. However, specific diagnostic techniques for feline samples are scarce. In this study, we standardized an in-house indirect enzyme-linked immunosorbent assay (ELISA) using cr...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Santa Maria
2024-03-01
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| Series: | Ciência Rural |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782024000800503&lng=en&tlng=en |
| _version_ | 1797244706543894528 |
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| author | Gabriela Döwich Pradella Taiane Acunha Escobar Thália Pacheco dos Santos Jennifer Stello Minuzzi Lívia Kmetzsch Rosa e Silva Isac Junior Roman Fernanda Silveira Flôres Vogel Claudia Acosta Duarte Irina Lübeck |
| author_facet | Gabriela Döwich Pradella Taiane Acunha Escobar Thália Pacheco dos Santos Jennifer Stello Minuzzi Lívia Kmetzsch Rosa e Silva Isac Junior Roman Fernanda Silveira Flôres Vogel Claudia Acosta Duarte Irina Lübeck |
| author_sort | Gabriela Döwich Pradella |
| collection | DOAJ |
| description | ABSTRACT: Feline leishmaniosis is infrequent worldwide, and cats have been suggested as secondary reservoirs for the parasite. However, specific diagnostic techniques for feline samples are scarce. In this study, we standardized an in-house indirect enzyme-linked immunosorbent assay (ELISA) using crude Leishmaniainfantum antigen to detect antibodies in feline samples from an endemic canine visceral leishmaniosis (CVL) area in the western border of Brazil. The results were compared with those of an indirect immunofluorescence assay (IFA). We tested semi-domiciled felines residing in Uruguaiana and Barra do Quaraí, Rio Grande do Sul. Among the 41 samples, 25 (61%) were positive using ELISA and 24 (58%) were positive using IFA (1:40). Our findings demonstrated a high seropositivity of feline samples from the endemic CVL area in the western border of Brazil, and we proposed the use of an in-house ELISA with crude antigen for population screening. This is the first serological survey on felines in a region where CVL is well established. |
| first_indexed | 2024-04-24T19:15:16Z |
| format | Article |
| id | doaj.art-7d8e25d5f3bf4118be59b9c25d9939c8 |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-04-24T19:15:16Z |
| publishDate | 2024-03-01 |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-7d8e25d5f3bf4118be59b9c25d9939c82024-03-26T07:40:50ZengUniversidade Federal de Santa MariaCiência Rural1678-45962024-03-0154810.1590/0103-8478cr20230127Detection of antibodies against Leishmania species using enzyme-linked immunosorbent assay in cats from the western border of BrazilGabriela Döwich Pradellahttps://orcid.org/0000-0002-5386-8706Taiane Acunha Escobarhttps://orcid.org/0000-0001-8896-3271Thália Pacheco dos Santoshttps://orcid.org/0009-0004-8869-8593Jennifer Stello MinuzziLívia Kmetzsch Rosa e Silvahttps://orcid.org/0000-0003-3766-5869Isac Junior Romanhttps://orcid.org/0000-0002-1974-4769Fernanda Silveira Flôres Vogelhttps://orcid.org/0000-0003-1351-9912Claudia Acosta Duartehttps://orcid.org/0000-0001-8044-4274Irina Lübeckhttps://orcid.org/0000-0002-9241-3376ABSTRACT: Feline leishmaniosis is infrequent worldwide, and cats have been suggested as secondary reservoirs for the parasite. However, specific diagnostic techniques for feline samples are scarce. In this study, we standardized an in-house indirect enzyme-linked immunosorbent assay (ELISA) using crude Leishmaniainfantum antigen to detect antibodies in feline samples from an endemic canine visceral leishmaniosis (CVL) area in the western border of Brazil. The results were compared with those of an indirect immunofluorescence assay (IFA). We tested semi-domiciled felines residing in Uruguaiana and Barra do Quaraí, Rio Grande do Sul. Among the 41 samples, 25 (61%) were positive using ELISA and 24 (58%) were positive using IFA (1:40). Our findings demonstrated a high seropositivity of feline samples from the endemic CVL area in the western border of Brazil, and we proposed the use of an in-house ELISA with crude antigen for population screening. This is the first serological survey on felines in a region where CVL is well established.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782024000800503&lng=en&tlng=enfelineleishmaniasisserodiagnosisimmunodiagnosticLeishmania infantum |
| spellingShingle | Gabriela Döwich Pradella Taiane Acunha Escobar Thália Pacheco dos Santos Jennifer Stello Minuzzi Lívia Kmetzsch Rosa e Silva Isac Junior Roman Fernanda Silveira Flôres Vogel Claudia Acosta Duarte Irina Lübeck Detection of antibodies against Leishmania species using enzyme-linked immunosorbent assay in cats from the western border of Brazil Ciência Rural feline leishmaniasis serodiagnosis immunodiagnostic Leishmania infantum |
| title | Detection of antibodies against Leishmania species using enzyme-linked immunosorbent assay in cats from the western border of Brazil |
| title_full | Detection of antibodies against Leishmania species using enzyme-linked immunosorbent assay in cats from the western border of Brazil |
| title_fullStr | Detection of antibodies against Leishmania species using enzyme-linked immunosorbent assay in cats from the western border of Brazil |
| title_full_unstemmed | Detection of antibodies against Leishmania species using enzyme-linked immunosorbent assay in cats from the western border of Brazil |
| title_short | Detection of antibodies against Leishmania species using enzyme-linked immunosorbent assay in cats from the western border of Brazil |
| title_sort | detection of antibodies against leishmania species using enzyme linked immunosorbent assay in cats from the western border of brazil |
| topic | feline leishmaniasis serodiagnosis immunodiagnostic Leishmania infantum |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782024000800503&lng=en&tlng=en |
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