Novel Nanoarchitectures Based on Lignin Nanoparticles for Electrochemical Eco-Friendly Biosensing Development
Novel nanoarchitectures based on lignin nanoparticles (LNPs) were designed and realized for electrochemical eco-friendly biosensing development. Two types of lignin nanoparticles were utilized for the modification of a gold bare electrode, namely organosolv (OLNPs) and kraft lignin (KLNPs) nanoparti...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/3/718 |
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| author | Cristina Tortolini Eliana Capecchi Federico Tasca Riccardo Pofi Mary Anna Venneri Raffaele Saladino Riccarda Antiochia |
| author_facet | Cristina Tortolini Eliana Capecchi Federico Tasca Riccardo Pofi Mary Anna Venneri Raffaele Saladino Riccarda Antiochia |
| author_sort | Cristina Tortolini |
| collection | DOAJ |
| description | Novel nanoarchitectures based on lignin nanoparticles (LNPs) were designed and realized for electrochemical eco-friendly biosensing development. Two types of lignin nanoparticles were utilized for the modification of a gold bare electrode, namely organosolv (OLNPs) and kraft lignin (KLNPs) nanoparticles, synthetized from a sulfur-free and a sulfur lignin, respectively. The electrochemical behavior of LNP-modified electrodes was studied using two electrochemical techniques, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Compared to the gold bare electrode, an evident decrease in the faradaic current and increase of the ΔE<sub>p</sub> were observed in cyclic voltammograms. In addition, larger semicircles were registered in Nyquist plots. These results suggest a strong inhibition effect of the electron transfer reaction by LNPs layer, especially in the case of KLNPs. The modified electrodes, properly assembled with concanavalin A (ConA) and glucose oxidase (GOx), were successively tested as biosensing platforms for glucose, showing a sensitivity of (4.53 ± 0.467) and (13.74 ± 1.84) μA mM<sup>−1</sup> cm<sup>2</sup> for Au/SAMCys/OLNPs/ConA/GOx and Au/KLNPs/ConA/GOx biosensors, respectively. Finally, different layers of the KNLPs/ConA/GOx-modified Au electrode were tested, and the three-layered Au(KNLPs/ConA/GOx)<sub>3</sub> showed the best analytical performance. |
| first_indexed | 2024-03-10T13:17:28Z |
| format | Article |
| id | doaj.art-7d8e8247314a447e9550eaf4be9c40a9 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T13:17:28Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-7d8e8247314a447e9550eaf4be9c40a92023-11-21T10:16:08ZengMDPI AGNanomaterials2079-49912021-03-0111371810.3390/nano11030718Novel Nanoarchitectures Based on Lignin Nanoparticles for Electrochemical Eco-Friendly Biosensing DevelopmentCristina Tortolini0Eliana Capecchi1Federico Tasca2Riccardo Pofi3Mary Anna Venneri4Raffaele Saladino5Riccarda Antiochia6Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, ItalyDepartment of Biological and Ecological Sciences, University of Tuscia, Via s. Camillo de Lellis snc, 01100 Viterbo, ItalyDepartamento de Química de los Materiales, Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40, Correo 33, Sucursal Matucana, Santiago 9170022, ChileDepartment of Experimental Medicine, Sapienza University of Rome, Viale Regina Elena 324, 00166 Rome, ItalyDepartment of Experimental Medicine, Sapienza University of Rome, Viale Regina Elena 324, 00166 Rome, ItalyDepartment of Biological and Ecological Sciences, University of Tuscia, Via s. Camillo de Lellis snc, 01100 Viterbo, ItalyDepartment of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, ItalyNovel nanoarchitectures based on lignin nanoparticles (LNPs) were designed and realized for electrochemical eco-friendly biosensing development. Two types of lignin nanoparticles were utilized for the modification of a gold bare electrode, namely organosolv (OLNPs) and kraft lignin (KLNPs) nanoparticles, synthetized from a sulfur-free and a sulfur lignin, respectively. The electrochemical behavior of LNP-modified electrodes was studied using two electrochemical techniques, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Compared to the gold bare electrode, an evident decrease in the faradaic current and increase of the ΔE<sub>p</sub> were observed in cyclic voltammograms. In addition, larger semicircles were registered in Nyquist plots. These results suggest a strong inhibition effect of the electron transfer reaction by LNPs layer, especially in the case of KLNPs. The modified electrodes, properly assembled with concanavalin A (ConA) and glucose oxidase (GOx), were successively tested as biosensing platforms for glucose, showing a sensitivity of (4.53 ± 0.467) and (13.74 ± 1.84) μA mM<sup>−1</sup> cm<sup>2</sup> for Au/SAMCys/OLNPs/ConA/GOx and Au/KLNPs/ConA/GOx biosensors, respectively. Finally, different layers of the KNLPs/ConA/GOx-modified Au electrode were tested, and the three-layered Au(KNLPs/ConA/GOx)<sub>3</sub> showed the best analytical performance.https://www.mdpi.com/2079-4991/11/3/718lignin nanoparticlesorganosolv ligninkraft ligninelectrochemical platformglucose biosensor |
| spellingShingle | Cristina Tortolini Eliana Capecchi Federico Tasca Riccardo Pofi Mary Anna Venneri Raffaele Saladino Riccarda Antiochia Novel Nanoarchitectures Based on Lignin Nanoparticles for Electrochemical Eco-Friendly Biosensing Development Nanomaterials lignin nanoparticles organosolv lignin kraft lignin electrochemical platform glucose biosensor |
| title | Novel Nanoarchitectures Based on Lignin Nanoparticles for Electrochemical Eco-Friendly Biosensing Development |
| title_full | Novel Nanoarchitectures Based on Lignin Nanoparticles for Electrochemical Eco-Friendly Biosensing Development |
| title_fullStr | Novel Nanoarchitectures Based on Lignin Nanoparticles for Electrochemical Eco-Friendly Biosensing Development |
| title_full_unstemmed | Novel Nanoarchitectures Based on Lignin Nanoparticles for Electrochemical Eco-Friendly Biosensing Development |
| title_short | Novel Nanoarchitectures Based on Lignin Nanoparticles for Electrochemical Eco-Friendly Biosensing Development |
| title_sort | novel nanoarchitectures based on lignin nanoparticles for electrochemical eco friendly biosensing development |
| topic | lignin nanoparticles organosolv lignin kraft lignin electrochemical platform glucose biosensor |
| url | https://www.mdpi.com/2079-4991/11/3/718 |
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