Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids
Summary: We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected tumors and adjacent tissues, and bodily fluids,...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720302124 |
| _version_ | 1819163698386698240 |
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| author | Linda Bojmar Han Sang Kim Gabriel C. Tobias Fanny A. Pelissier Vatter Serena Lucotti Kofi Ennu Gyan Candia M. Kenific Zurong Wan Kyung-A Kim DooA Kim Jonathan Hernandez Virginia Pascual Todd E. Heaton Michael P. La Quaglia David Kelsen Tanya M. Trippett David R. Jones William R. Jarnagin Irina R. Matei Haiying Zhang Ayuko Hoshino David Lyden |
| author_facet | Linda Bojmar Han Sang Kim Gabriel C. Tobias Fanny A. Pelissier Vatter Serena Lucotti Kofi Ennu Gyan Candia M. Kenific Zurong Wan Kyung-A Kim DooA Kim Jonathan Hernandez Virginia Pascual Todd E. Heaton Michael P. La Quaglia David Kelsen Tanya M. Trippett David R. Jones William R. Jarnagin Irina R. Matei Haiying Zhang Ayuko Hoshino David Lyden |
| author_sort | Linda Bojmar |
| collection | DOAJ |
| description | Summary: We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected tumors and adjacent tissues, and bodily fluids, such as blood, lymphatic fluid, and bile. The diversity of these samples requires robust and highly reproducible protocols and refined isolation technology, such as asymmetric-flow field-flow fractionation (AF4). Our isolation protocol allows for preparation of EVPs for various downstream applications, including proteomic profiling.For complete details on the use and execution of this protocol, please refer to Hoshino et al. (2020). |
| first_indexed | 2024-12-22T17:48:16Z |
| format | Article |
| id | doaj.art-7d8e9011528240a59bd11296b6bfba77 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-22T17:48:16Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-7d8e9011528240a59bd11296b6bfba772022-12-21T18:18:15ZengElsevierSTAR Protocols2666-16672021-03-0121100225Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluidsLinda Bojmar0Han Sang Kim1Gabriel C. Tobias2Fanny A. Pelissier Vatter3Serena Lucotti4Kofi Ennu Gyan5Candia M. Kenific6Zurong Wan7Kyung-A Kim8DooA Kim9Jonathan Hernandez10Virginia Pascual11Todd E. Heaton12Michael P. La Quaglia13David Kelsen14Tanya M. Trippett15David R. Jones16William R. Jarnagin17Irina R. Matei18Haiying Zhang19Ayuko Hoshino20David Lyden21Children’s Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children’s Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA; Department of Biomedical and Clinical Sciences, Linköping University, Linköping 58183, SwedenChildren’s Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children’s Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA; Yonsei Cancer Center, Division of Medical Oncology, Department of Internal Medicine, Graduate School of Medical Science, Brain Korea 21 Project, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, KoreaChildren’s Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children’s Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA; School of Physical Education and Sport, University of Sao Paulo, Sao Paulo, BrazilChildren’s Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children’s Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USAChildren’s Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children’s Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USAChildren’s Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children’s Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA; Tri-Institutional PhD Program in Computational Biology and Medicine, New York, NY, USAChildren’s Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children’s Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USAChildren’s Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children’s Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA; Department of Pediatrics, Weill Cornell Medicine, New York, NY, USAYonsei Cancer Center, Division of Medical Oncology, Department of Internal Medicine, Graduate School of Medical Science, Brain Korea 21 Project, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, KoreaYonsei Cancer Center, Division of Medical Oncology, Department of Internal Medicine, Graduate School of Medical Science, Brain Korea 21 Project, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, KoreaHepatopancreatobiliary Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA; Surgical Oncology Program, National Cancer Institute, National Institutes of Health, Bethesda, MD, USADepartment of Pediatrics, Weill Cornell Medicine, New York, NY, USA; Drukier Institute for Children's Health, Weill Cornell Medicine, New York, NY, USADepartment of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, NY, USA; Pediatric Surgical Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USADepartment of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, NY, USA; Pediatric Surgical Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USADepartment of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USADepartment of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, NY, USAThoracic Surgery Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USAHepatopancreatobiliary Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USAChildren’s Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children’s Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USAChildren’s Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children’s Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA; Corresponding authorChildren’s Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children’s Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA; School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan; Corresponding authorChildren’s Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children’s Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA; Corresponding authorSummary: We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected tumors and adjacent tissues, and bodily fluids, such as blood, lymphatic fluid, and bile. The diversity of these samples requires robust and highly reproducible protocols and refined isolation technology, such as asymmetric-flow field-flow fractionation (AF4). Our isolation protocol allows for preparation of EVPs for various downstream applications, including proteomic profiling.For complete details on the use and execution of this protocol, please refer to Hoshino et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720302124Cell cultureCell isolationCell MembraneExosomesExomeresExtracellular vesicles and particles |
| spellingShingle | Linda Bojmar Han Sang Kim Gabriel C. Tobias Fanny A. Pelissier Vatter Serena Lucotti Kofi Ennu Gyan Candia M. Kenific Zurong Wan Kyung-A Kim DooA Kim Jonathan Hernandez Virginia Pascual Todd E. Heaton Michael P. La Quaglia David Kelsen Tanya M. Trippett David R. Jones William R. Jarnagin Irina R. Matei Haiying Zhang Ayuko Hoshino David Lyden Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids STAR Protocols Cell culture Cell isolation Cell Membrane Exosomes Exomeres Extracellular vesicles and particles |
| title | Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids |
| title_full | Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids |
| title_fullStr | Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids |
| title_full_unstemmed | Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids |
| title_short | Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids |
| title_sort | extracellular vesicle and particle isolation from human and murine cell lines tissues and bodily fluids |
| topic | Cell culture Cell isolation Cell Membrane Exosomes Exomeres Extracellular vesicles and particles |
| url | http://www.sciencedirect.com/science/article/pii/S2666166720302124 |
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