Identification of putative fecundity-related gustatory receptor genes in the brown planthopper Nilaparvata lugens
Abstract Background The insect gustatory system plays a central role in the regulation of multiple physiological behaviors and the co-evolution between insects and their hosts. The gustatory receptors (Gr) are important to allow insects to sense their environment. It is critical to the selection of...
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BMC
2018-12-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-018-5391-5 |
| _version_ | 1828821366670884864 |
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| author | Kui Kang Pan Yang Li-E Chen Rui Pang Lu-Jun Yu Wen-Wu Zhou Zeng-Rong Zhu Wen-Qing Zhang |
| author_facet | Kui Kang Pan Yang Li-E Chen Rui Pang Lu-Jun Yu Wen-Wu Zhou Zeng-Rong Zhu Wen-Qing Zhang |
| author_sort | Kui Kang |
| collection | DOAJ |
| description | Abstract Background The insect gustatory system plays a central role in the regulation of multiple physiological behaviors and the co-evolution between insects and their hosts. The gustatory receptors (Gr) are important to allow insects to sense their environment. It is critical to the selection of foods, mates and oviposition sites of insects. In this study, the Gr family genes of the brown planthopper (BPH) Nilaparvata lugens Stål (Hemiptera: Delphacidae) were identified and analyzed, and their potential relationship to the fecundity of BPH was explored by RNA interference (RNAi). Results We identified 32 putative Gr genes by analyzing transcriptome and genome data from BPH. Most of these Gr proteins have the typical structure of seven transmembrane domains. The BPH Gr genes (NlGrs) were expressed in virtually all tissues and stages, whilst higher transcript accumulations were found in adult stages and in the midguts of females. Based on the phylogenic analysis, we classified NlGrs into five potential categories, including 2 sugar receptors, 2 Gr43a-like receptors, 7 CO2 receptors, 5 bitter receptors and 13 NlGrs with unknown functions. Moreover, we found that 10 NlGrs have at least two alternative splicing variants, and obtained alternative splicing isoforms of 5 NlGrs. Finally, RNAi of 29 NlGrs showed that 27 of them are related to the transcript levels of two fecundity related genes vitellogenin and vitellogenin receptor. Conclusions We found 32 Gr genes in BPH, among which at least 27 are required for normal expression of fecundity markers of this insect pest. These findings provide the basis for the functional study of Grs and the exploration of potential genes involved in the monophagous character of BPH. |
| first_indexed | 2024-12-12T12:53:01Z |
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| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-12T12:53:01Z |
| publishDate | 2018-12-01 |
| publisher | BMC |
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| series | BMC Genomics |
| spelling | doaj.art-7d90a6eb134b4d71972c51e6117952152022-12-22T00:23:57ZengBMCBMC Genomics1471-21642018-12-0119111210.1186/s12864-018-5391-5Identification of putative fecundity-related gustatory receptor genes in the brown planthopper Nilaparvata lugensKui Kang0Pan Yang1Li-E Chen2Rui Pang3Lu-Jun Yu4Wen-Wu Zhou5Zeng-Rong Zhu6Wen-Qing Zhang7State Key Laboratory of Biocontrol and School of Life Sciences, Sun Yat-sen UniversityState Key Laboratory of Biocontrol and School of Life Sciences, Sun Yat-sen UniversityState Key Laboratory of Biocontrol and School of Life Sciences, Sun Yat-sen UniversityState Key Laboratory of Biocontrol and School of Life Sciences, Sun Yat-sen UniversityState Key Laboratory of Biocontrol and School of Life Sciences, Sun Yat-sen UniversityState Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang UniversityState Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang UniversityState Key Laboratory of Biocontrol and School of Life Sciences, Sun Yat-sen UniversityAbstract Background The insect gustatory system plays a central role in the regulation of multiple physiological behaviors and the co-evolution between insects and their hosts. The gustatory receptors (Gr) are important to allow insects to sense their environment. It is critical to the selection of foods, mates and oviposition sites of insects. In this study, the Gr family genes of the brown planthopper (BPH) Nilaparvata lugens Stål (Hemiptera: Delphacidae) were identified and analyzed, and their potential relationship to the fecundity of BPH was explored by RNA interference (RNAi). Results We identified 32 putative Gr genes by analyzing transcriptome and genome data from BPH. Most of these Gr proteins have the typical structure of seven transmembrane domains. The BPH Gr genes (NlGrs) were expressed in virtually all tissues and stages, whilst higher transcript accumulations were found in adult stages and in the midguts of females. Based on the phylogenic analysis, we classified NlGrs into five potential categories, including 2 sugar receptors, 2 Gr43a-like receptors, 7 CO2 receptors, 5 bitter receptors and 13 NlGrs with unknown functions. Moreover, we found that 10 NlGrs have at least two alternative splicing variants, and obtained alternative splicing isoforms of 5 NlGrs. Finally, RNAi of 29 NlGrs showed that 27 of them are related to the transcript levels of two fecundity related genes vitellogenin and vitellogenin receptor. Conclusions We found 32 Gr genes in BPH, among which at least 27 are required for normal expression of fecundity markers of this insect pest. These findings provide the basis for the functional study of Grs and the exploration of potential genes involved in the monophagous character of BPH.http://link.springer.com/article/10.1186/s12864-018-5391-5Nilaparvata lugensGustatory receptorAlternative splicingFecundity |
| spellingShingle | Kui Kang Pan Yang Li-E Chen Rui Pang Lu-Jun Yu Wen-Wu Zhou Zeng-Rong Zhu Wen-Qing Zhang Identification of putative fecundity-related gustatory receptor genes in the brown planthopper Nilaparvata lugens BMC Genomics Nilaparvata lugens Gustatory receptor Alternative splicing Fecundity |
| title | Identification of putative fecundity-related gustatory receptor genes in the brown planthopper Nilaparvata lugens |
| title_full | Identification of putative fecundity-related gustatory receptor genes in the brown planthopper Nilaparvata lugens |
| title_fullStr | Identification of putative fecundity-related gustatory receptor genes in the brown planthopper Nilaparvata lugens |
| title_full_unstemmed | Identification of putative fecundity-related gustatory receptor genes in the brown planthopper Nilaparvata lugens |
| title_short | Identification of putative fecundity-related gustatory receptor genes in the brown planthopper Nilaparvata lugens |
| title_sort | identification of putative fecundity related gustatory receptor genes in the brown planthopper nilaparvata lugens |
| topic | Nilaparvata lugens Gustatory receptor Alternative splicing Fecundity |
| url | http://link.springer.com/article/10.1186/s12864-018-5391-5 |
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