A Decade of CRISPR-Cas Gnome Editing in <i>C. elegans</i>
CRISPR-Cas allows us to introduce desired genome editing, including mutations, epitopes, and deletions, with unprecedented efficiency. The development of CRISPR-Cas has progressed to such an extent that it is now applicable in various fields, with the help of model organisms. <i>C. elegans<...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/24/15863 |
| _version_ | 1797457268818575360 |
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| author | Hyun-Min Kim Yebin Hong Jiani Chen |
| author_facet | Hyun-Min Kim Yebin Hong Jiani Chen |
| author_sort | Hyun-Min Kim |
| collection | DOAJ |
| description | CRISPR-Cas allows us to introduce desired genome editing, including mutations, epitopes, and deletions, with unprecedented efficiency. The development of CRISPR-Cas has progressed to such an extent that it is now applicable in various fields, with the help of model organisms. <i>C. elegans</i> is one of the pioneering animals in which numerous CRISPR-Cas strategies have been rapidly established over the past decade. Ironically, the emergence of numerous methods makes the choice of the correct method difficult. Choosing an appropriate selection or screening approach is the first step in planning a genome modification. This report summarizes the key features and applications of CRISPR-Cas methods using <i>C. elegans</i>, illustrating key strategies. Our overview of significant advances in CRISPR-Cas will help readers understand the current advances in genome editing and navigate various methods of CRISPR-Cas genome editing. |
| first_indexed | 2024-03-09T16:19:47Z |
| format | Article |
| id | doaj.art-7d90cb1a3bb64ab59bc6b390a9c35cea |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:19:47Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7d90cb1a3bb64ab59bc6b390a9c35cea2023-11-24T15:29:20ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0123241586310.3390/ijms232415863A Decade of CRISPR-Cas Gnome Editing in <i>C. elegans</i>Hyun-Min Kim0Yebin Hong1Jiani Chen2Division of Natural and Applied Sciences, Duke Kunshan University, Kunshan 215316, ChinaDivision of Natural and Applied Sciences, Duke Kunshan University, Kunshan 215316, ChinaDivision of Natural and Applied Sciences, Duke Kunshan University, Kunshan 215316, ChinaCRISPR-Cas allows us to introduce desired genome editing, including mutations, epitopes, and deletions, with unprecedented efficiency. The development of CRISPR-Cas has progressed to such an extent that it is now applicable in various fields, with the help of model organisms. <i>C. elegans</i> is one of the pioneering animals in which numerous CRISPR-Cas strategies have been rapidly established over the past decade. Ironically, the emergence of numerous methods makes the choice of the correct method difficult. Choosing an appropriate selection or screening approach is the first step in planning a genome modification. This report summarizes the key features and applications of CRISPR-Cas methods using <i>C. elegans</i>, illustrating key strategies. Our overview of significant advances in CRISPR-Cas will help readers understand the current advances in genome editing and navigate various methods of CRISPR-Cas genome editing.https://www.mdpi.com/1422-0067/23/24/15863CRISPRCasgenome editing<i>C. elegans</i>genome engineering |
| spellingShingle | Hyun-Min Kim Yebin Hong Jiani Chen A Decade of CRISPR-Cas Gnome Editing in <i>C. elegans</i> International Journal of Molecular Sciences CRISPR Cas genome editing <i>C. elegans</i> genome engineering |
| title | A Decade of CRISPR-Cas Gnome Editing in <i>C. elegans</i> |
| title_full | A Decade of CRISPR-Cas Gnome Editing in <i>C. elegans</i> |
| title_fullStr | A Decade of CRISPR-Cas Gnome Editing in <i>C. elegans</i> |
| title_full_unstemmed | A Decade of CRISPR-Cas Gnome Editing in <i>C. elegans</i> |
| title_short | A Decade of CRISPR-Cas Gnome Editing in <i>C. elegans</i> |
| title_sort | decade of crispr cas gnome editing in i c elegans i |
| topic | CRISPR Cas genome editing <i>C. elegans</i> genome engineering |
| url | https://www.mdpi.com/1422-0067/23/24/15863 |
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