A High-Fat Diet Increases the Characteristics of Gut Microbial Composition and the Intestinal Damage Associated with Non-Alcoholic Fatty Liver Disease
The prevalence of non-alcoholic fatty liver disease (NAFLD) is increasing annually, and emerging evidence suggests that the gut microbiota plays a causative role in the development of NAFLD. However, the role of gut microbiota in the development of NAFLD remains unclear and warrants further investig...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/23/16733 |
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| author | Xiaoyang Zhu Jiajia Cai Yifu Wang Xinyu Liu Xiaolei Chen Haifei Wang Zhengchang Wu Wenbin Bao Hairui Fan Shenglong Wu |
| author_facet | Xiaoyang Zhu Jiajia Cai Yifu Wang Xinyu Liu Xiaolei Chen Haifei Wang Zhengchang Wu Wenbin Bao Hairui Fan Shenglong Wu |
| author_sort | Xiaoyang Zhu |
| collection | DOAJ |
| description | The prevalence of non-alcoholic fatty liver disease (NAFLD) is increasing annually, and emerging evidence suggests that the gut microbiota plays a causative role in the development of NAFLD. However, the role of gut microbiota in the development of NAFLD remains unclear and warrants further investigation. Thus, C57BL/6J mice were fed a high-fat diet (HFD), and we found that the HFD significantly induced obesity and increased the accumulation of intrahepatic lipids, along with alterations in serum biochemical parameters. Moreover, it was observed that the HFD also impaired gut barrier integrity. It was revealed via 16S rRNA gene sequencing that the HFD increased gut microbial diversity, which enriched <i>Colidextribacter</i>, <i>Lachnospiraceae-NK4A136-group</i>, <i>Acetatifactor</i>, and <i>Erysipelatoclostridium</i>. Meanwhile, it reduced the abundance of <i>Faecalibaculum</i>, <i>Muribaculaceae</i>, and <i>Coriobacteriaceae-UCG-002</i>. The predicted metabolic pathways suggest that HFD enhances the chemotaxis and functional activity of gut microbiota pathways associated with flagellar assembly, while also increasing the risk of intestinal pathogen colonization and inflammation. And the phosphotransferase system, streptomycin biosynthesis, and starch/sucrose metabolism exhibited decreases. These findings reveal the composition and predictive functions of the intestinal microbiome in NAFLD, further corroborating the association between gut microbiota and NAFLD while providing novel insights into its potential application in gut microbiome research for NAFLD patients. |
| first_indexed | 2024-03-09T01:49:57Z |
| format | Article |
| id | doaj.art-7d91dee5bd7f4f44b3e27a13a0e8d1d3 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T01:49:57Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7d91dee5bd7f4f44b3e27a13a0e8d1d32023-12-08T15:16:56ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124231673310.3390/ijms242316733A High-Fat Diet Increases the Characteristics of Gut Microbial Composition and the Intestinal Damage Associated with Non-Alcoholic Fatty Liver DiseaseXiaoyang Zhu0Jiajia Cai1Yifu Wang2Xinyu Liu3Xiaolei Chen4Haifei Wang5Zhengchang Wu6Wenbin Bao7Hairui Fan8Shenglong Wu9Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaThe prevalence of non-alcoholic fatty liver disease (NAFLD) is increasing annually, and emerging evidence suggests that the gut microbiota plays a causative role in the development of NAFLD. However, the role of gut microbiota in the development of NAFLD remains unclear and warrants further investigation. Thus, C57BL/6J mice were fed a high-fat diet (HFD), and we found that the HFD significantly induced obesity and increased the accumulation of intrahepatic lipids, along with alterations in serum biochemical parameters. Moreover, it was observed that the HFD also impaired gut barrier integrity. It was revealed via 16S rRNA gene sequencing that the HFD increased gut microbial diversity, which enriched <i>Colidextribacter</i>, <i>Lachnospiraceae-NK4A136-group</i>, <i>Acetatifactor</i>, and <i>Erysipelatoclostridium</i>. Meanwhile, it reduced the abundance of <i>Faecalibaculum</i>, <i>Muribaculaceae</i>, and <i>Coriobacteriaceae-UCG-002</i>. The predicted metabolic pathways suggest that HFD enhances the chemotaxis and functional activity of gut microbiota pathways associated with flagellar assembly, while also increasing the risk of intestinal pathogen colonization and inflammation. And the phosphotransferase system, streptomycin biosynthesis, and starch/sucrose metabolism exhibited decreases. These findings reveal the composition and predictive functions of the intestinal microbiome in NAFLD, further corroborating the association between gut microbiota and NAFLD while providing novel insights into its potential application in gut microbiome research for NAFLD patients.https://www.mdpi.com/1422-0067/24/23/16733non-alcoholic fatty liver disease16S rRNA-seqgut microbiotabiodiversitygut barrier |
| spellingShingle | Xiaoyang Zhu Jiajia Cai Yifu Wang Xinyu Liu Xiaolei Chen Haifei Wang Zhengchang Wu Wenbin Bao Hairui Fan Shenglong Wu A High-Fat Diet Increases the Characteristics of Gut Microbial Composition and the Intestinal Damage Associated with Non-Alcoholic Fatty Liver Disease International Journal of Molecular Sciences non-alcoholic fatty liver disease 16S rRNA-seq gut microbiota biodiversity gut barrier |
| title | A High-Fat Diet Increases the Characteristics of Gut Microbial Composition and the Intestinal Damage Associated with Non-Alcoholic Fatty Liver Disease |
| title_full | A High-Fat Diet Increases the Characteristics of Gut Microbial Composition and the Intestinal Damage Associated with Non-Alcoholic Fatty Liver Disease |
| title_fullStr | A High-Fat Diet Increases the Characteristics of Gut Microbial Composition and the Intestinal Damage Associated with Non-Alcoholic Fatty Liver Disease |
| title_full_unstemmed | A High-Fat Diet Increases the Characteristics of Gut Microbial Composition and the Intestinal Damage Associated with Non-Alcoholic Fatty Liver Disease |
| title_short | A High-Fat Diet Increases the Characteristics of Gut Microbial Composition and the Intestinal Damage Associated with Non-Alcoholic Fatty Liver Disease |
| title_sort | high fat diet increases the characteristics of gut microbial composition and the intestinal damage associated with non alcoholic fatty liver disease |
| topic | non-alcoholic fatty liver disease 16S rRNA-seq gut microbiota biodiversity gut barrier |
| url | https://www.mdpi.com/1422-0067/24/23/16733 |
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