Cooperative recruitment of FtsW to the division site of Bacillus subtilis
Five essential proteins are known to assemble at the division site of Bacillus subtilis. However, the recruitment of the FtsW homolog is still unclear. Here, by taking advantage of GFP fusions and spore germination, we show that the assembly of FtsW is interdependent with the localization of PBP 2B...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-11-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01808/full |
| _version_ | 1818838585112002560 |
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| author | Pamela Gamba Leendert Hamoen Richard A Daniel |
| author_facet | Pamela Gamba Leendert Hamoen Richard A Daniel |
| author_sort | Pamela Gamba |
| collection | DOAJ |
| description | Five essential proteins are known to assemble at the division site of Bacillus subtilis. However, the recruitment of the FtsW homolog is still unclear. Here, by taking advantage of GFP fusions and spore germination, we show that the assembly of FtsW is interdependent with the localization of PBP 2B and FtsL. Interestingly, the Z-ring appeared to disassemble upon prolonged depletion of late division proteins. Nevertheless, we could restore Z-ring formation and constriction by re-inducing FtsW, which suggests that the stability of the Z-ring is stimulated by the assembly of a functional division complex. |
| first_indexed | 2024-12-19T03:40:44Z |
| format | Article |
| id | doaj.art-7d99595e694e465c90f9b61d4c135280 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-19T03:40:44Z |
| publishDate | 2016-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-7d99595e694e465c90f9b61d4c1352802022-12-21T20:37:13ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-11-01710.3389/fmicb.2016.01808219573Cooperative recruitment of FtsW to the division site of Bacillus subtilisPamela Gamba0Leendert Hamoen1Richard A Daniel2Newcastle UniversityUinversity of AmsterdamNewcastle UniversityFive essential proteins are known to assemble at the division site of Bacillus subtilis. However, the recruitment of the FtsW homolog is still unclear. Here, by taking advantage of GFP fusions and spore germination, we show that the assembly of FtsW is interdependent with the localization of PBP 2B and FtsL. Interestingly, the Z-ring appeared to disassemble upon prolonged depletion of late division proteins. Nevertheless, we could restore Z-ring formation and constriction by re-inducing FtsW, which suggests that the stability of the Z-ring is stimulated by the assembly of a functional division complex.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01808/fullBacillus subtilisCell DivisionftsZFTSWFtsL |
| spellingShingle | Pamela Gamba Leendert Hamoen Richard A Daniel Cooperative recruitment of FtsW to the division site of Bacillus subtilis Frontiers in Microbiology Bacillus subtilis Cell Division ftsZ FTSW FtsL |
| title | Cooperative recruitment of FtsW to the division site of Bacillus subtilis |
| title_full | Cooperative recruitment of FtsW to the division site of Bacillus subtilis |
| title_fullStr | Cooperative recruitment of FtsW to the division site of Bacillus subtilis |
| title_full_unstemmed | Cooperative recruitment of FtsW to the division site of Bacillus subtilis |
| title_short | Cooperative recruitment of FtsW to the division site of Bacillus subtilis |
| title_sort | cooperative recruitment of ftsw to the division site of bacillus subtilis |
| topic | Bacillus subtilis Cell Division ftsZ FTSW FtsL |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01808/full |
| work_keys_str_mv | AT pamelagamba cooperativerecruitmentofftswtothedivisionsiteofbacillussubtilis AT leenderthamoen cooperativerecruitmentofftswtothedivisionsiteofbacillussubtilis AT richardadaniel cooperativerecruitmentofftswtothedivisionsiteofbacillussubtilis |