Metabolic Implications of Using BioOrthogonal Non-Canonical Amino Acid Tagging (BONCAT) for Tracking Protein Synthesis

Metabolic Implications of Using BioOrthogonal Non-Canonical Amino Acid Tagging (BONCAT) for Tracking Protein Synthesis

BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT) is a powerful tool for tracking protein synthesis on the level of single cells within communities and whole organisms. A basic premise of BONCAT is that the non-canonical amino acids (NCAA) used to track translational activity do not significan...

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Bibliographic Details
Main Authors: Katherine F. Steward, Brian Eilers, Brian Tripet, Amanda Fuchs, Michael Dorle, Rachel Rawle, Berliza Soriano, Narayanaganesh Balasubramanian, Valérie Copié, Brian Bothner, Roland Hatzenpichler
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-02-01
Series:Frontiers in Microbiology
Subjects:
metabolomics
BONCAT
non-canonical amino acids
L-azidohomoalanine
L-homopropargylglycine
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2020.00197/full

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