| Summary: | We investigated the changes in the physiological and biochemical properties of potato plants exposed to differing ozone (O<sub>3</sub>) concentrations (5 ppm, 10 ppm) and exposure times (2, 4, 8, 12, 16 min) to determine the safe dose that could be used in crop protection programs. We measured the gas exchange, relative chlorophyll content, chlorophyll fluorescence and total antioxidant capacity in potato leaves exposed to O<sub>3</sub> fumigation. The fresh weight (FW) of the aboveground part of the plants and a visual assessment of plant condition were determined after the end of the experiment. The plants were given two O<sub>3</sub> treatments and measurements were carried out four times: on the 1st and 7th day after treatment. We conclude that O<sub>3</sub> exposure time had a greater impact on the reduction in the efficiency of the potato photosynthetic apparatus than O<sub>3</sub> concentration. Research has showed that O<sub>3</sub> in 5 ppm concentration for 2 and 4 min and 10 ppm for 2 min increased the efficiency of the photosynthesis and antioxidant activity in leaf processes, and these doses could be taken into account in further research on the potential for using O<sub>3</sub> in potato protection.
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