Anti-inflammatory cytokine stimulation of HMC3 cells: Proteome dataset
The immunoprotective functions of microglia in the brain are mediated by the inflammatory M1 phenotype. This phenotype is challenged by anti-inflammatory cytokines which polarize the microglia cells to an immunosuppressive M2 phenotype, a trait that is often exploited by cancer cells to evade immune...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-08-01
|
| Series: | Data in Brief |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340923005334 |
| _version_ | 1797743994950647808 |
|---|---|
| author | Shreya Ahuja Iulia M. Lazar |
| author_facet | Shreya Ahuja Iulia M. Lazar |
| author_sort | Shreya Ahuja |
| collection | DOAJ |
| description | The immunoprotective functions of microglia in the brain are mediated by the inflammatory M1 phenotype. This phenotype is challenged by anti-inflammatory cytokines which polarize the microglia cells to an immunosuppressive M2 phenotype, a trait that is often exploited by cancer cells to evade immune recognition and promote tumor growth. Investigating the molecular determinants of this behavior is crucial for advancing the understanding of the mechanisms that cancer cells use to escape immune attack. In this article, we describe liquid chromatography (LC)-mass spectrometry (MS)/proteomic data acquired with an EASY-nanoLC 1200-Q ExactiveTM OrbitrapTM mass spectrometer that reflect the response of human microglia cells (HMC3) to stimulation with potential cancer-released anti-inflammatory cytokines known to be key players in promoting tumorigenesis in the brain (IL-4, IL-13, IL-10, TGFB and MCP-1). The MS files were processed with the Proteome Discoverer v.2.4 software package. The cell culture conditions, the sample preparation protocols, the MS acquisition parameters, and the data processing approach are described in detail. The RAW and processed MS files associated with this work were deposited in the PRIDE partner repository of the ProteomeXchange Consortium with the dataset identifiers PXD023163 and PXD023166, and the analyzed data in the Mendeley Data cloud-based repository with DOI 10.17632/fvhw2zwt5d.1. The biological interpretation of the data can be accessed in the research article “Systems-Level Proteomics Evaluation of Microglia Response to Tumor-Supportive Anti-inflammatory Cytokines” (Shreya Ahuja and Iulia M. Lazar, Frontiers in Immunology 2021 [1]). The proteome data described in this article will benefit researchers who are either interested in re-processing the data with alternative search engines and filtering criteria, and/or exploring the data in more depth to advance the understanding of cancer progression and the discovery of novel biomarkers or drug targets. |
| first_indexed | 2024-03-12T15:04:27Z |
| format | Article |
| id | doaj.art-7d9bc6faed1d45fea2e82df3c0371c4f |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-03-12T15:04:27Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-7d9bc6faed1d45fea2e82df3c0371c4f2023-08-13T04:54:18ZengElsevierData in Brief2352-34092023-08-0149109433Anti-inflammatory cytokine stimulation of HMC3 cells: Proteome datasetShreya Ahuja0Iulia M. Lazar1Department of Biological Sciences, Virgina Tech, USADepartment of Biological Sciences, Virgina Tech, USA; Fralin Life Sciences Institute, Virgina Tech, USA; Academy of Integrated Sciences/Systems Biology, Virgina Tech, USA; Carilion School of Medicine, Virginia Tech, 1981 Kraft Drive, Blacksburg, VA 24061, USA; Corresponding author at: Department of Biological Sciences, Virgina Tech, USA.The immunoprotective functions of microglia in the brain are mediated by the inflammatory M1 phenotype. This phenotype is challenged by anti-inflammatory cytokines which polarize the microglia cells to an immunosuppressive M2 phenotype, a trait that is often exploited by cancer cells to evade immune recognition and promote tumor growth. Investigating the molecular determinants of this behavior is crucial for advancing the understanding of the mechanisms that cancer cells use to escape immune attack. In this article, we describe liquid chromatography (LC)-mass spectrometry (MS)/proteomic data acquired with an EASY-nanoLC 1200-Q ExactiveTM OrbitrapTM mass spectrometer that reflect the response of human microglia cells (HMC3) to stimulation with potential cancer-released anti-inflammatory cytokines known to be key players in promoting tumorigenesis in the brain (IL-4, IL-13, IL-10, TGFB and MCP-1). The MS files were processed with the Proteome Discoverer v.2.4 software package. The cell culture conditions, the sample preparation protocols, the MS acquisition parameters, and the data processing approach are described in detail. The RAW and processed MS files associated with this work were deposited in the PRIDE partner repository of the ProteomeXchange Consortium with the dataset identifiers PXD023163 and PXD023166, and the analyzed data in the Mendeley Data cloud-based repository with DOI 10.17632/fvhw2zwt5d.1. The biological interpretation of the data can be accessed in the research article “Systems-Level Proteomics Evaluation of Microglia Response to Tumor-Supportive Anti-inflammatory Cytokines” (Shreya Ahuja and Iulia M. Lazar, Frontiers in Immunology 2021 [1]). The proteome data described in this article will benefit researchers who are either interested in re-processing the data with alternative search engines and filtering criteria, and/or exploring the data in more depth to advance the understanding of cancer progression and the discovery of novel biomarkers or drug targets.http://www.sciencedirect.com/science/article/pii/S2352340923005334MicrogliaAnti-inflammatory cytokinesCancerProteomics |
| spellingShingle | Shreya Ahuja Iulia M. Lazar Anti-inflammatory cytokine stimulation of HMC3 cells: Proteome dataset Data in Brief Microglia Anti-inflammatory cytokines Cancer Proteomics |
| title | Anti-inflammatory cytokine stimulation of HMC3 cells: Proteome dataset |
| title_full | Anti-inflammatory cytokine stimulation of HMC3 cells: Proteome dataset |
| title_fullStr | Anti-inflammatory cytokine stimulation of HMC3 cells: Proteome dataset |
| title_full_unstemmed | Anti-inflammatory cytokine stimulation of HMC3 cells: Proteome dataset |
| title_short | Anti-inflammatory cytokine stimulation of HMC3 cells: Proteome dataset |
| title_sort | anti inflammatory cytokine stimulation of hmc3 cells proteome dataset |
| topic | Microglia Anti-inflammatory cytokines Cancer Proteomics |
| url | http://www.sciencedirect.com/science/article/pii/S2352340923005334 |
| work_keys_str_mv | AT shreyaahuja antiinflammatorycytokinestimulationofhmc3cellsproteomedataset AT iuliamlazar antiinflammatorycytokinestimulationofhmc3cellsproteomedataset |