Protective influence of betaine on intestinal health by regulating inflammation and improving barrier function in broilers under heat stress
ABSTRACT: This research was executed to study the impacts of adding betaine (BT) to broiler diets on intestinal inflammatory response and barrier integrity under heat stress (HS). At 21 d of age, 150 male broilers (Ross 308) were randomly assigned to 3 treatment groups: control (CON) group, in which...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-09-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579121003710 |
| _version_ | 1818887233842708480 |
|---|---|
| author | Rashed A. Alhotan Ali R. Al Sulaiman Abdulrahman S. Alharthi Alaeldein M. Abudabos |
| author_facet | Rashed A. Alhotan Ali R. Al Sulaiman Abdulrahman S. Alharthi Alaeldein M. Abudabos |
| author_sort | Rashed A. Alhotan |
| collection | DOAJ |
| description | ABSTRACT: This research was executed to study the impacts of adding betaine (BT) to broiler diets on intestinal inflammatory response and barrier integrity under heat stress (HS). At 21 d of age, 150 male broilers (Ross 308) were randomly assigned to 3 treatment groups: control (CON) group, in which broilers were provided standard finisher feed under thermoneutral condition (22 ± 1°C); HS group and HS + BT group, in which broilers were given the standard feed supplied with 0 and 1,000 mg/kg BT, respectively, under cyclic HS condition (33 ± 1°C for 8 h from 08:00 to 16:00 h and the thermoneutral temperature for the residual hours). Each treatment was replicated ten times with 5 broilers per replicate. The HS group showed an elevation (P < 0.05) in serum corticosterone (CORT) concentration, D-lactate acid (D-LA) content, and diamine oxidase (DAO) activity, mucosal interleukin-1β (IL-1β) level, and expression of heat shock protein 70 (HSP70) gene, and a reduction (P < 0.05) in mucosal interleukin-10 (IL-10) level and secretory immunoglobulin A (SIgA) content and relative abundance of mRNA for occludin (OCLN), zonula occludens-1 (ZO-1), claudin-1 (CLDN1), and claudin-4 (CLDN4). In contrast, broilers in the HS + BT group exhibited a raise (P < 0.05) in mucosal IL-10 level and SIgA content and relative expression of OCLN and ZO-1 genes, and a decline (P < 0.05) in serum CORT concentration and DAO activity, mucosal IL-1β level, and expression of HSP70 mRNA. These results indicate that supplemental BT can ameliorate intestinal injury in heat-challenged broilers by suppressing inflammatory responses and enhancing mucosal barrier function. |
| first_indexed | 2024-12-19T16:33:59Z |
| format | Article |
| id | doaj.art-7d9bd2cf7fef4ba4af51622686b20ff2 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-19T16:33:59Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-7d9bd2cf7fef4ba4af51622686b20ff22022-12-21T20:14:03ZengElsevierPoultry Science0032-57912021-09-011009101337Protective influence of betaine on intestinal health by regulating inflammation and improving barrier function in broilers under heat stressRashed A. Alhotan0Ali R. Al Sulaiman1Abdulrahman S. Alharthi2Alaeldein M. Abudabos3Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaNational Center for Environmental Technology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology, P.O. Box 6086, Riyadh 11442, Saudi ArabiaDepartment of Animal Production, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaDepartment of Animal Production, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia; Corresponding author:ABSTRACT: This research was executed to study the impacts of adding betaine (BT) to broiler diets on intestinal inflammatory response and barrier integrity under heat stress (HS). At 21 d of age, 150 male broilers (Ross 308) were randomly assigned to 3 treatment groups: control (CON) group, in which broilers were provided standard finisher feed under thermoneutral condition (22 ± 1°C); HS group and HS + BT group, in which broilers were given the standard feed supplied with 0 and 1,000 mg/kg BT, respectively, under cyclic HS condition (33 ± 1°C for 8 h from 08:00 to 16:00 h and the thermoneutral temperature for the residual hours). Each treatment was replicated ten times with 5 broilers per replicate. The HS group showed an elevation (P < 0.05) in serum corticosterone (CORT) concentration, D-lactate acid (D-LA) content, and diamine oxidase (DAO) activity, mucosal interleukin-1β (IL-1β) level, and expression of heat shock protein 70 (HSP70) gene, and a reduction (P < 0.05) in mucosal interleukin-10 (IL-10) level and secretory immunoglobulin A (SIgA) content and relative abundance of mRNA for occludin (OCLN), zonula occludens-1 (ZO-1), claudin-1 (CLDN1), and claudin-4 (CLDN4). In contrast, broilers in the HS + BT group exhibited a raise (P < 0.05) in mucosal IL-10 level and SIgA content and relative expression of OCLN and ZO-1 genes, and a decline (P < 0.05) in serum CORT concentration and DAO activity, mucosal IL-1β level, and expression of HSP70 mRNA. These results indicate that supplemental BT can ameliorate intestinal injury in heat-challenged broilers by suppressing inflammatory responses and enhancing mucosal barrier function.http://www.sciencedirect.com/science/article/pii/S0032579121003710betaineheat stressintestinal inflammationintestinal barrier functionbroiler |
| spellingShingle | Rashed A. Alhotan Ali R. Al Sulaiman Abdulrahman S. Alharthi Alaeldein M. Abudabos Protective influence of betaine on intestinal health by regulating inflammation and improving barrier function in broilers under heat stress Poultry Science betaine heat stress intestinal inflammation intestinal barrier function broiler |
| title | Protective influence of betaine on intestinal health by regulating inflammation and improving barrier function in broilers under heat stress |
| title_full | Protective influence of betaine on intestinal health by regulating inflammation and improving barrier function in broilers under heat stress |
| title_fullStr | Protective influence of betaine on intestinal health by regulating inflammation and improving barrier function in broilers under heat stress |
| title_full_unstemmed | Protective influence of betaine on intestinal health by regulating inflammation and improving barrier function in broilers under heat stress |
| title_short | Protective influence of betaine on intestinal health by regulating inflammation and improving barrier function in broilers under heat stress |
| title_sort | protective influence of betaine on intestinal health by regulating inflammation and improving barrier function in broilers under heat stress |
| topic | betaine heat stress intestinal inflammation intestinal barrier function broiler |
| url | http://www.sciencedirect.com/science/article/pii/S0032579121003710 |
| work_keys_str_mv | AT rashedaalhotan protectiveinfluenceofbetaineonintestinalhealthbyregulatinginflammationandimprovingbarrierfunctioninbroilersunderheatstress AT aliralsulaiman protectiveinfluenceofbetaineonintestinalhealthbyregulatinginflammationandimprovingbarrierfunctioninbroilersunderheatstress AT abdulrahmansalharthi protectiveinfluenceofbetaineonintestinalhealthbyregulatinginflammationandimprovingbarrierfunctioninbroilersunderheatstress AT alaeldeinmabudabos protectiveinfluenceofbetaineonintestinalhealthbyregulatinginflammationandimprovingbarrierfunctioninbroilersunderheatstress |