A Comparative Study of Helicobacter pylori Growth on Different Agar-based Media
Background/Aims: Optimal culture conditions for Helicobacter pylori have not been established. We compared the effectiveness of four different agar-based media for the growth of H. pylori. Materials and Methods: G27, ATCC #43504 and 60190, and primary cultured strains were used. H. pylori strains we...
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| Format: | Article |
| Language: | English |
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Yong Chan Lee
2017-12-01
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| Series: | The Korean Journal of Helicobacter and Upper Gastrointestinal Research |
| Subjects: | |
| Online Access: | http://www.helicojournal.org/journal/view.html?doi=10.7704/kjhugr.2017.17.4.208 |
| _version_ | 1818933547749081088 |
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| author | Jung Hwan Lee Jiwan Park Mi Ri Park Yoon Hee Na Soo-Jeong Cho |
| author_facet | Jung Hwan Lee Jiwan Park Mi Ri Park Yoon Hee Na Soo-Jeong Cho |
| author_sort | Jung Hwan Lee |
| collection | DOAJ |
| description | Background/Aims: Optimal culture conditions for Helicobacter pylori have not been established. We compared the effectiveness of four different agar-based media for the growth of H. pylori. Materials and Methods: G27, ATCC #43504 and 60190, and primary cultured strains were used. H. pylori strains were cultured for four days under four culture conditions: chocolate agar, Thayer-Martin (TM) agar containing vancomycin-colistin-nystatin inhibitor (VCNI), Brucella agar, and brain heart infusion (BHI) agar containing 5% horse blood and IsoVitaleX (BBL™ BD, USA). Culture of cells in each medium was repeated fourteen times. The growth of H. pylori was measured by using a spectrophotometer. Results: TM, Brucella, and BHI agars showed mean absorbance values of 0.099, 0.059, 1.410, and 0.913, respectively. These values were significantly different (P=0.030). After post-adjustment by Bonferroni correction, similar growth was noted for in chocolate, Brucella, and BHI agars; however, TM agar significantly suppressed H. pylori growth compared with Brucella agar (P=0.031). Conclusions: Chocolate, Brucella, and BHI agars provided effective culture conditions for the growth of H. pylori. TM agar containing VCNI suppressed the growth of H. pylori and other organisms. |
| first_indexed | 2024-12-20T04:50:07Z |
| format | Article |
| id | doaj.art-7d9dd647d68c44118409a5884c6a5b47 |
| institution | Directory Open Access Journal |
| issn | 1738-3331 |
| language | English |
| last_indexed | 2024-12-20T04:50:07Z |
| publishDate | 2017-12-01 |
| publisher | Yong Chan Lee |
| record_format | Article |
| series | The Korean Journal of Helicobacter and Upper Gastrointestinal Research |
| spelling | doaj.art-7d9dd647d68c44118409a5884c6a5b472022-12-21T19:52:53ZengYong Chan LeeThe Korean Journal of Helicobacter and Upper Gastrointestinal Research1738-33312017-12-0117420821210.7704/kjhugr.2017.17.4.208kjhugr.2017.17.4.208A Comparative Study of Helicobacter pylori Growth on Different Agar-based MediaJung Hwan Lee0Jiwan Park1Mi Ri Park2Yoon Hee Na3Soo-Jeong Cho4Center for Gastric Cancer, National Cancer Center, Goyang, KoreaCenter for Gastric Cancer, National Cancer Center, Goyang, KoreaCenter for Gastric Cancer, National Cancer Center, Goyang, KoreaCenter for Gastric Cancer, National Cancer Center, Goyang, KoreaCenter for Gastric Cancer, National Cancer Center, Goyang, KoreaBackground/Aims: Optimal culture conditions for Helicobacter pylori have not been established. We compared the effectiveness of four different agar-based media for the growth of H. pylori. Materials and Methods: G27, ATCC #43504 and 60190, and primary cultured strains were used. H. pylori strains were cultured for four days under four culture conditions: chocolate agar, Thayer-Martin (TM) agar containing vancomycin-colistin-nystatin inhibitor (VCNI), Brucella agar, and brain heart infusion (BHI) agar containing 5% horse blood and IsoVitaleX (BBL™ BD, USA). Culture of cells in each medium was repeated fourteen times. The growth of H. pylori was measured by using a spectrophotometer. Results: TM, Brucella, and BHI agars showed mean absorbance values of 0.099, 0.059, 1.410, and 0.913, respectively. These values were significantly different (P=0.030). After post-adjustment by Bonferroni correction, similar growth was noted for in chocolate, Brucella, and BHI agars; however, TM agar significantly suppressed H. pylori growth compared with Brucella agar (P=0.031). Conclusions: Chocolate, Brucella, and BHI agars provided effective culture conditions for the growth of H. pylori. TM agar containing VCNI suppressed the growth of H. pylori and other organisms.http://www.helicojournal.org/journal/view.html?doi=10.7704/kjhugr.2017.17.4.208AgarCultureHelicobacter pyloriMedia |
| spellingShingle | Jung Hwan Lee Jiwan Park Mi Ri Park Yoon Hee Na Soo-Jeong Cho A Comparative Study of Helicobacter pylori Growth on Different Agar-based Media The Korean Journal of Helicobacter and Upper Gastrointestinal Research Agar Culture Helicobacter pylori Media |
| title | A Comparative Study of Helicobacter pylori Growth on Different Agar-based Media |
| title_full | A Comparative Study of Helicobacter pylori Growth on Different Agar-based Media |
| title_fullStr | A Comparative Study of Helicobacter pylori Growth on Different Agar-based Media |
| title_full_unstemmed | A Comparative Study of Helicobacter pylori Growth on Different Agar-based Media |
| title_short | A Comparative Study of Helicobacter pylori Growth on Different Agar-based Media |
| title_sort | comparative study of helicobacter pylori growth on different agar based media |
| topic | Agar Culture Helicobacter pylori Media |
| url | http://www.helicojournal.org/journal/view.html?doi=10.7704/kjhugr.2017.17.4.208 |
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