Effect of Cytotoxic T-Lymphocyte Antigen-4 on the Efficacy of the Fatty Acid-Binding Protein Vaccine Against Schistosoma japonicum
The present study evaluated the impact of blocking cytotoxic T-lymphocyte antigen-4 (CTLA-4) activity on the protective effect elicited by the fatty acid binding protein (FABP) vaccine against Schistosoma japonicum infection. Mice were randomly divided into uninfected, infected control, anti-CTLA-4...
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Frontiers Media S.A.
2019-05-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.01022/full |
| _version_ | 1818040015250259968 |
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| author | Chun-lian Tang Qun Pan Ya-ping Xie Ying Xiong Rong-hui Zhang Jin Huang |
| author_facet | Chun-lian Tang Qun Pan Ya-ping Xie Ying Xiong Rong-hui Zhang Jin Huang |
| author_sort | Chun-lian Tang |
| collection | DOAJ |
| description | The present study evaluated the impact of blocking cytotoxic T-lymphocyte antigen-4 (CTLA-4) activity on the protective effect elicited by the fatty acid binding protein (FABP) vaccine against Schistosoma japonicum infection. Mice were randomly divided into uninfected, infected control, anti-CTLA-4 monoclonal antibody (anti-CTLA-4 mAb), FABP, and combination (anti-CTLA-4 mAb and FABP) groups. An assessment of the S. japonicum worm and egg burden in the infected mice revealed that the worm reduction-rate induced by FABP administration was increased from 26.58 to 54.61% by co-administration of the monoclonal anti-CTLA antibody (anti-CTLA-4 mAb). Furthermore, the regulatory T cell (Treg) percentage was significantly increased in mice after administration of the anti-CTLA-4 mAb, but not the FABP vaccine, and elevated levels of the cytokines interferon (IFN)-γ, interleukin (IL)-2, IL-4, and IL-5 were observed in infected mice that were administered the anti-CTLA-4 mAb. Notably, the diameter of egg granulomas in the anti-CTLA-4 mAb and combination groups was significantly increased compared to that observed in the infected control group. Together, these results suggest that co-administering the FABP vaccine and anti-CTLA-4 treatment may have synergistically increased the immunoprotective effect of the FABP vaccine by promoting T-helper 1-type immune responses, while incurring increased tissue damage. |
| first_indexed | 2024-12-10T08:07:48Z |
| format | Article |
| id | doaj.art-7d9e4840377040babbec42ed48ede800 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-10T08:07:48Z |
| publishDate | 2019-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-7d9e4840377040babbec42ed48ede8002022-12-22T01:56:38ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-05-011010.3389/fimmu.2019.01022448036Effect of Cytotoxic T-Lymphocyte Antigen-4 on the Efficacy of the Fatty Acid-Binding Protein Vaccine Against Schistosoma japonicumChun-lian Tang0Qun Pan1Ya-ping Xie2Ying Xiong3Rong-hui Zhang4Jin Huang5Wuchang Hospital, Affiliated to Wuhan University of Science and Technology, Wuhan, ChinaWuchang Hospital, Affiliated to Wuhan University of Science and Technology, Wuhan, ChinaWuchang Hospital, Affiliated to Wuhan University of Science and Technology, Wuhan, ChinaWuchang Hospital, Affiliated to Wuhan University of Science and Technology, Wuhan, ChinaWuchang Hospital, Affiliated to Wuhan University of Science and Technology, Wuhan, ChinaDepartment of Clinical Laboratory, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaThe present study evaluated the impact of blocking cytotoxic T-lymphocyte antigen-4 (CTLA-4) activity on the protective effect elicited by the fatty acid binding protein (FABP) vaccine against Schistosoma japonicum infection. Mice were randomly divided into uninfected, infected control, anti-CTLA-4 monoclonal antibody (anti-CTLA-4 mAb), FABP, and combination (anti-CTLA-4 mAb and FABP) groups. An assessment of the S. japonicum worm and egg burden in the infected mice revealed that the worm reduction-rate induced by FABP administration was increased from 26.58 to 54.61% by co-administration of the monoclonal anti-CTLA antibody (anti-CTLA-4 mAb). Furthermore, the regulatory T cell (Treg) percentage was significantly increased in mice after administration of the anti-CTLA-4 mAb, but not the FABP vaccine, and elevated levels of the cytokines interferon (IFN)-γ, interleukin (IL)-2, IL-4, and IL-5 were observed in infected mice that were administered the anti-CTLA-4 mAb. Notably, the diameter of egg granulomas in the anti-CTLA-4 mAb and combination groups was significantly increased compared to that observed in the infected control group. Together, these results suggest that co-administering the FABP vaccine and anti-CTLA-4 treatment may have synergistically increased the immunoprotective effect of the FABP vaccine by promoting T-helper 1-type immune responses, while incurring increased tissue damage.https://www.frontiersin.org/article/10.3389/fimmu.2019.01022/fullSchistosoma japonicumfatty acid-binding proteinregulatory T cellscytotoxic T-lymphocyte antigen-4granuloma |
| spellingShingle | Chun-lian Tang Qun Pan Ya-ping Xie Ying Xiong Rong-hui Zhang Jin Huang Effect of Cytotoxic T-Lymphocyte Antigen-4 on the Efficacy of the Fatty Acid-Binding Protein Vaccine Against Schistosoma japonicum Frontiers in Immunology Schistosoma japonicum fatty acid-binding protein regulatory T cells cytotoxic T-lymphocyte antigen-4 granuloma |
| title | Effect of Cytotoxic T-Lymphocyte Antigen-4 on the Efficacy of the Fatty Acid-Binding Protein Vaccine Against Schistosoma japonicum |
| title_full | Effect of Cytotoxic T-Lymphocyte Antigen-4 on the Efficacy of the Fatty Acid-Binding Protein Vaccine Against Schistosoma japonicum |
| title_fullStr | Effect of Cytotoxic T-Lymphocyte Antigen-4 on the Efficacy of the Fatty Acid-Binding Protein Vaccine Against Schistosoma japonicum |
| title_full_unstemmed | Effect of Cytotoxic T-Lymphocyte Antigen-4 on the Efficacy of the Fatty Acid-Binding Protein Vaccine Against Schistosoma japonicum |
| title_short | Effect of Cytotoxic T-Lymphocyte Antigen-4 on the Efficacy of the Fatty Acid-Binding Protein Vaccine Against Schistosoma japonicum |
| title_sort | effect of cytotoxic t lymphocyte antigen 4 on the efficacy of the fatty acid binding protein vaccine against schistosoma japonicum |
| topic | Schistosoma japonicum fatty acid-binding protein regulatory T cells cytotoxic T-lymphocyte antigen-4 granuloma |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.01022/full |
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