Molecular crosstalk between articular cartilage, meniscus, synovium, and subchondral bone in osteoarthritis
AimsOsteoarthritis (OA) is a common degenerative joint disease worldwide, which is characterized by articular cartilage lesions. With more understanding of the disease, OA is considered to be a disorder of the whole joint. However, molecular communication within and between tissues during the diseas...
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| Format: | Article |
| Language: | English |
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The British Editorial Society of Bone & Joint Surgery
2022-12-01
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| Series: | Bone & Joint Research |
| Subjects: | |
| Online Access: | https://online.boneandjoint.org.uk/doi/10.1302/2046-3758.1112.BJR-2022-0215.R1 |
| _version_ | 1797980585690398720 |
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| author | Maochun Wang Guihua Tan Huiming Jiang Anlong Liu Rui Wu Jiawei Li Ziying Sun Zhongyang Lv Wei Sun Dongquan Shi |
| author_facet | Maochun Wang Guihua Tan Huiming Jiang Anlong Liu Rui Wu Jiawei Li Ziying Sun Zhongyang Lv Wei Sun Dongquan Shi |
| author_sort | Maochun Wang |
| collection | DOAJ |
| description | AimsOsteoarthritis (OA) is a common degenerative joint disease worldwide, which is characterized by articular cartilage lesions. With more understanding of the disease, OA is considered to be a disorder of the whole joint. However, molecular communication within and between tissues during the disease process is still unclear. In this study, we used transcriptome data to reveal crosstalk between different tissues in OA.MethodsWe used four groups of transcription profiles acquired from the Gene Expression Omnibus database, including articular cartilage, meniscus, synovium, and subchondral bone, to screen differentially expressed genes during OA. Potential crosstalk between tissues was depicted by ligand-receptor pairs.ResultsDuring OA, there were 626, 97, 1,060, and 2,330 differentially expressed genes in articular cartilage, meniscus, synovium, and subchondral bone, respectively. Gene Ontology enrichment revealed that these genes were enriched in extracellular matrix and structure organization, ossification, neutrophil degranulation, and activation at different degrees. Through ligand-receptor pairing and proteome of OA synovial fluid, we predicted ligand-receptor interactions and constructed a crosstalk atlas of the whole joint. Several interactions were reproduced by transwell experiment in chondrocytes and synovial cells, including TNC-NT5E, TNC-SDC4, FN1-ITGA5, and FN1-NT5E. After lipopolysaccharide (LPS) or interleukin (IL)-1β stimulation, the ligand expression of chondrocytes and synovial cells was upregulated, and corresponding receptors of co-culture cells were also upregulated.ConclusionEach tissue displayed a different expression pattern in transcriptome, demonstrating their specific roles in OA. We highlighted tissue molecular crosstalk through ligand-receptor pairs in OA pathophysiology, and generated a crosstalk atlas. Strategies to interfere with these candidate ligands and receptors may help to discover molecular targets for future OA therapy.Cite this article: Bone Joint Res 2022;11(12):862–872. |
| first_indexed | 2024-04-11T05:56:26Z |
| format | Article |
| id | doaj.art-7da10e8ee74249fa90d39a58710316e1 |
| institution | Directory Open Access Journal |
| issn | 2046-3758 |
| language | English |
| last_indexed | 2024-04-11T05:56:26Z |
| publishDate | 2022-12-01 |
| publisher | The British Editorial Society of Bone & Joint Surgery |
| record_format | Article |
| series | Bone & Joint Research |
| spelling | doaj.art-7da10e8ee74249fa90d39a58710316e12022-12-22T04:41:52ZengThe British Editorial Society of Bone & Joint SurgeryBone & Joint Research2046-37582022-12-01111286287210.1302/2046-3758.1112.BJR-2022-0215.R1Molecular crosstalk between articular cartilage, meniscus, synovium, and subchondral bone in osteoarthritisMaochun Wang0Guihua Tan1Huiming Jiang2Anlong Liu3Rui Wu4Jiawei Li5Ziying Sun6Zhongyang Lv7Wei Sun8Dongquan Shi9State Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, ChinaDepartment of Orthopedics, The Affiliated Jiangyin Hospital of Southeast University Medical College, Wuxi, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, ChinaAimsOsteoarthritis (OA) is a common degenerative joint disease worldwide, which is characterized by articular cartilage lesions. With more understanding of the disease, OA is considered to be a disorder of the whole joint. However, molecular communication within and between tissues during the disease process is still unclear. In this study, we used transcriptome data to reveal crosstalk between different tissues in OA.MethodsWe used four groups of transcription profiles acquired from the Gene Expression Omnibus database, including articular cartilage, meniscus, synovium, and subchondral bone, to screen differentially expressed genes during OA. Potential crosstalk between tissues was depicted by ligand-receptor pairs.ResultsDuring OA, there were 626, 97, 1,060, and 2,330 differentially expressed genes in articular cartilage, meniscus, synovium, and subchondral bone, respectively. Gene Ontology enrichment revealed that these genes were enriched in extracellular matrix and structure organization, ossification, neutrophil degranulation, and activation at different degrees. Through ligand-receptor pairing and proteome of OA synovial fluid, we predicted ligand-receptor interactions and constructed a crosstalk atlas of the whole joint. Several interactions were reproduced by transwell experiment in chondrocytes and synovial cells, including TNC-NT5E, TNC-SDC4, FN1-ITGA5, and FN1-NT5E. After lipopolysaccharide (LPS) or interleukin (IL)-1β stimulation, the ligand expression of chondrocytes and synovial cells was upregulated, and corresponding receptors of co-culture cells were also upregulated.ConclusionEach tissue displayed a different expression pattern in transcriptome, demonstrating their specific roles in OA. We highlighted tissue molecular crosstalk through ligand-receptor pairs in OA pathophysiology, and generated a crosstalk atlas. Strategies to interfere with these candidate ligands and receptors may help to discover molecular targets for future OA therapy.Cite this article: Bone Joint Res 2022;11(12):862–872.https://online.boneandjoint.org.uk/doi/10.1302/2046-3758.1112.BJR-2022-0215.R1OsteoarthritisCrosstalkTranscriptomeDifferentially expressed genesLigand-receptorarticular cartilage |
| spellingShingle | Maochun Wang Guihua Tan Huiming Jiang Anlong Liu Rui Wu Jiawei Li Ziying Sun Zhongyang Lv Wei Sun Dongquan Shi Molecular crosstalk between articular cartilage, meniscus, synovium, and subchondral bone in osteoarthritis Bone & Joint Research Osteoarthritis Crosstalk Transcriptome Differentially expressed genes Ligand-receptor articular cartilage |
| title | Molecular crosstalk between articular cartilage, meniscus, synovium, and subchondral bone in osteoarthritis |
| title_full | Molecular crosstalk between articular cartilage, meniscus, synovium, and subchondral bone in osteoarthritis |
| title_fullStr | Molecular crosstalk between articular cartilage, meniscus, synovium, and subchondral bone in osteoarthritis |
| title_full_unstemmed | Molecular crosstalk between articular cartilage, meniscus, synovium, and subchondral bone in osteoarthritis |
| title_short | Molecular crosstalk between articular cartilage, meniscus, synovium, and subchondral bone in osteoarthritis |
| title_sort | molecular crosstalk between articular cartilage meniscus synovium and subchondral bone in osteoarthritis |
| topic | Osteoarthritis Crosstalk Transcriptome Differentially expressed genes Ligand-receptor articular cartilage |
| url | https://online.boneandjoint.org.uk/doi/10.1302/2046-3758.1112.BJR-2022-0215.R1 |
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