Potential role of Citrus bergamia flower essential oil against oral pathogens
Abstract Background Oral bacterial infections are difficult to treat due to emergence of resistance against antibiotic therapy. Essential oils are considered emerging alternate therapy against bacterial infections and biofilms. We investigated Citrus bergemia flower essential oil against oral pathog...
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | BMC Complementary Medicine and Therapies |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12906-024-04457-7 |
| _version_ | 1827284455335657472 |
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| author | Muhammad Imran Aziz Muhammad Mohtasheemul Hasan Riaz Ullah Ahmed Bari Mohsin Abbas Khan Syed Zia Ul Hasnain Rabia Baloch Muhammad Akram Aqsa obaid Aziz Ullah Khizar Abbas Adnan Amin |
| author_facet | Muhammad Imran Aziz Muhammad Mohtasheemul Hasan Riaz Ullah Ahmed Bari Mohsin Abbas Khan Syed Zia Ul Hasnain Rabia Baloch Muhammad Akram Aqsa obaid Aziz Ullah Khizar Abbas Adnan Amin |
| author_sort | Muhammad Imran Aziz |
| collection | DOAJ |
| description | Abstract Background Oral bacterial infections are difficult to treat due to emergence of resistance against antibiotic therapy. Essential oils are considered emerging alternate therapy against bacterial infections and biofilms. We investigated Citrus bergemia flower essential oil against oral pathogens. Methods The essential oil was analsyed using Gas Chromatography(GC–MS), in silico investigations, antioxidant, antimicrobial, antibiofilm and antiquorum sensing assays. Results Gas Chromatography analysis confirmed presence of 17 compounds including 1,6-Octadien-3-ol,3,7-dimethyl, 48.17%), l-limonene (22.03%) and p-menth-1-ol, 8-ol (7.31%) as major components. In silico analysis showed compliance of all tested major components with Lipinski’s rule, Bioavailability and antimicrobial activity using PASS (prediction of activity spectrum of substances). Molecular docking with transcriptional regulators 3QP5, 5OE3, 4B2O and 3Q3D revealed strong interaction of all tested compounds except 1,6-Octadien-3-ol,3,7-dimethyl. All tested compounds presented significant inhibition of DPPH (2,2-diphenyl-1-picrylhydrazyl) (IC50 0.65 mg/mL), H2O2 (hydrogen peroxide) (63.5%) and high FRAP (ferrous reducing antioxidant power) value (239.01 µg). In antimicrobial screening a significant activity (MIC 0.125 mg/mL) against Bacillus paramycoides and Bacillus chungangensis was observed. Likewise a strong antibiofilm (52.1 – 69.5%) and anti-QS (quorum sensing) (4–16 mm) activity was recorded in a dose dependent manner. Conclusion It was therefore concluded that C. bergemia essential oil posess strong antioxidant, antimicrobial and antibiofilm activities against tested oral pathogens. |
| first_indexed | 2024-04-24T09:56:46Z |
| format | Article |
| id | doaj.art-7da11abcb9ec4a708d61b791d3df5305 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-04-24T09:56:46Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-7da11abcb9ec4a708d61b791d3df53052024-04-14T11:07:49ZengBMCBMC Complementary Medicine and Therapies2662-76712024-04-0124111910.1186/s12906-024-04457-7Potential role of Citrus bergamia flower essential oil against oral pathogensMuhammad Imran Aziz0Muhammad Mohtasheemul Hasan1Riaz Ullah2Ahmed Bari3Mohsin Abbas Khan4Syed Zia Ul Hasnain5Rabia Baloch6Muhammad Akram7Aqsa obaid8Aziz Ullah9Khizar Abbas10Adnan Amin11Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, University of KarachiDepartment of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, University of KarachiDepartment of Pharmacognosy, College of Pharmacy, King Saud UniversityDepartment of Pharmaceutical Chemistry, College of Pharmacy, King Saud UniversityDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of BahawalpurDepartment of Pharmacognosy, Faculty of Pharmacy, Bahauddin Zakaria UniversityAllama Iqbal Teaching HospitalPakistan Council for Scientific and Industrial Research (PCSIR)NPRL, Department of Pharmacognosy, Faculty of Pharmacy, Gomal University, 29050Pukyong National UniversityDepartment of Pharmacognosy, Faculty of Pharmacy, Bahauddin Zakaria UniversityNPRL, Department of Pharmacognosy, Faculty of Pharmacy, Gomal University, 29050Abstract Background Oral bacterial infections are difficult to treat due to emergence of resistance against antibiotic therapy. Essential oils are considered emerging alternate therapy against bacterial infections and biofilms. We investigated Citrus bergemia flower essential oil against oral pathogens. Methods The essential oil was analsyed using Gas Chromatography(GC–MS), in silico investigations, antioxidant, antimicrobial, antibiofilm and antiquorum sensing assays. Results Gas Chromatography analysis confirmed presence of 17 compounds including 1,6-Octadien-3-ol,3,7-dimethyl, 48.17%), l-limonene (22.03%) and p-menth-1-ol, 8-ol (7.31%) as major components. In silico analysis showed compliance of all tested major components with Lipinski’s rule, Bioavailability and antimicrobial activity using PASS (prediction of activity spectrum of substances). Molecular docking with transcriptional regulators 3QP5, 5OE3, 4B2O and 3Q3D revealed strong interaction of all tested compounds except 1,6-Octadien-3-ol,3,7-dimethyl. All tested compounds presented significant inhibition of DPPH (2,2-diphenyl-1-picrylhydrazyl) (IC50 0.65 mg/mL), H2O2 (hydrogen peroxide) (63.5%) and high FRAP (ferrous reducing antioxidant power) value (239.01 µg). In antimicrobial screening a significant activity (MIC 0.125 mg/mL) against Bacillus paramycoides and Bacillus chungangensis was observed. Likewise a strong antibiofilm (52.1 – 69.5%) and anti-QS (quorum sensing) (4–16 mm) activity was recorded in a dose dependent manner. Conclusion It was therefore concluded that C. bergemia essential oil posess strong antioxidant, antimicrobial and antibiofilm activities against tested oral pathogens.https://doi.org/10.1186/s12906-024-04457-7Cell to cell signalingOxidative stressAromaIn silico analysisDrug resistance |
| spellingShingle | Muhammad Imran Aziz Muhammad Mohtasheemul Hasan Riaz Ullah Ahmed Bari Mohsin Abbas Khan Syed Zia Ul Hasnain Rabia Baloch Muhammad Akram Aqsa obaid Aziz Ullah Khizar Abbas Adnan Amin Potential role of Citrus bergamia flower essential oil against oral pathogens BMC Complementary Medicine and Therapies Cell to cell signaling Oxidative stress Aroma In silico analysis Drug resistance |
| title | Potential role of Citrus bergamia flower essential oil against oral pathogens |
| title_full | Potential role of Citrus bergamia flower essential oil against oral pathogens |
| title_fullStr | Potential role of Citrus bergamia flower essential oil against oral pathogens |
| title_full_unstemmed | Potential role of Citrus bergamia flower essential oil against oral pathogens |
| title_short | Potential role of Citrus bergamia flower essential oil against oral pathogens |
| title_sort | potential role of citrus bergamia flower essential oil against oral pathogens |
| topic | Cell to cell signaling Oxidative stress Aroma In silico analysis Drug resistance |
| url | https://doi.org/10.1186/s12906-024-04457-7 |
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