Size‐Controlled DNA Tile Self‐Assembly Nanostructures Through Caveolae‐Mediated Endocytosis for Signal‐Amplified Imaging of MicroRNAs in Living Cells
Abstract Signal‐amplified imaging of microRNAs (miRNAs) is a promising strategy at the single‐cell level because liquid biopsy fails to reflect real‐time dynamic miRNA levels. However, the internalization pathways for available conventional vectors predominantly involve endo‐lysosomes, showing nonid...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-07-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202300614 |
| _version_ | 1797770678037905408 |
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| author | Yanan Peng Zhijun Gao Bin Qiao Dongxia Li Huajie Pang Xiangde Lai Qiumei Pu Rui Zhang Xuan Zhao Guangyuan Zhao Dan Xu Yuanyuan Wang Yuxiang Ji Hua Pei Qiang Wu |
| author_facet | Yanan Peng Zhijun Gao Bin Qiao Dongxia Li Huajie Pang Xiangde Lai Qiumei Pu Rui Zhang Xuan Zhao Guangyuan Zhao Dan Xu Yuanyuan Wang Yuxiang Ji Hua Pei Qiang Wu |
| author_sort | Yanan Peng |
| collection | DOAJ |
| description | Abstract Signal‐amplified imaging of microRNAs (miRNAs) is a promising strategy at the single‐cell level because liquid biopsy fails to reflect real‐time dynamic miRNA levels. However, the internalization pathways for available conventional vectors predominantly involve endo‐lysosomes, showing nonideal cytoplasmic delivery efficiency. In this study, size‐controlled 9‐tile nanoarrays are designed and constructed by integrating catalytic hairpin assembly (CHA) with DNA tile self‐assembly technology to achieve caveolae‐mediated endocytosis for the amplified imaging of miRNAs in a complex intracellular environment. Compared with classical CHA, the 9‐tile nanoarrays possess high sensitivity and specificity for miRNAs, achieve excellent internalization efficiency by caveolar endocytosis, bypassing lysosomal traps, and exhibit more powerful signal‐amplified imaging of intracellular miRNAs. Because of their excellent safety, physiological stability, and highly efficient cytoplasmic delivery, the 9‐tile nanoarrays can realize real‐time amplified monitoring of miRNAs in various tumor and identical cells of different periods, and imaging effects are consistent with the actual expression levels of miRNAs, ultimately demonstrating their feasibility and capacity. This strategy provides a high‐potential delivery pathway for cell imaging and targeted delivery, simultaneously offering a meaningful reference for the application of DNA tile self‐assembly technology in relevant fundamental research and medical diagnostics. |
| first_indexed | 2024-03-12T21:26:39Z |
| format | Article |
| id | doaj.art-7da1a8b005244d91b291c4ddf38395f4 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-12T21:26:39Z |
| publishDate | 2023-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-7da1a8b005244d91b291c4ddf38395f42023-07-28T06:53:01ZengWileyAdvanced Science2198-38442023-07-011021n/an/a10.1002/advs.202300614Size‐Controlled DNA Tile Self‐Assembly Nanostructures Through Caveolae‐Mediated Endocytosis for Signal‐Amplified Imaging of MicroRNAs in Living CellsYanan Peng0Zhijun Gao1Bin Qiao2Dongxia Li3Huajie Pang4Xiangde Lai5Qiumei Pu6Rui Zhang7Xuan Zhao8Guangyuan Zhao9Dan Xu10Yuanyuan Wang11Yuxiang Ji12Hua Pei13Qiang Wu14The Second Affiliated Hospital School of Tropical Medicine Hainan Medical University Haikou 571199 P. R. ChinaThe Second Affiliated Hospital School of Tropical Medicine Hainan Medical University Haikou 571199 P. R. ChinaThe Second Affiliated Hospital School of Tropical Medicine Hainan Medical University Haikou 571199 P. R. ChinaThe Second Affiliated Hospital School of Tropical Medicine Hainan Medical University Haikou 571199 P. R. ChinaThe Second Affiliated Hospital School of Tropical Medicine Hainan Medical University Haikou 571199 P. R. ChinaThe Second Affiliated Hospital School of Tropical Medicine Hainan Medical University Haikou 571199 P. R. ChinaThe Second Affiliated Hospital School of Tropical Medicine Hainan Medical University Haikou 571199 P. R. ChinaThe Second Affiliated Hospital School of Tropical Medicine Hainan Medical University Haikou 571199 P. R. ChinaThe Second Affiliated Hospital School of Tropical Medicine Hainan Medical University Haikou 571199 P. R. ChinaThe Second Affiliated Hospital School of Tropical Medicine Hainan Medical University Haikou 571199 P. R. ChinaKey Laboratory of Tropical Translational Medicine of Ministry of Education School of Pharmacy Hainan Medical University Haikou 571199 P. R. ChinaThe Second Affiliated Hospital School of Tropical Medicine Hainan Medical University Haikou 571199 P. R. ChinaThe Second Affiliated Hospital School of Tropical Medicine Hainan Medical University Haikou 571199 P. R. ChinaThe Second Affiliated Hospital School of Tropical Medicine Hainan Medical University Haikou 571199 P. R. ChinaThe Second Affiliated Hospital School of Tropical Medicine Hainan Medical University Haikou 571199 P. R. ChinaAbstract Signal‐amplified imaging of microRNAs (miRNAs) is a promising strategy at the single‐cell level because liquid biopsy fails to reflect real‐time dynamic miRNA levels. However, the internalization pathways for available conventional vectors predominantly involve endo‐lysosomes, showing nonideal cytoplasmic delivery efficiency. In this study, size‐controlled 9‐tile nanoarrays are designed and constructed by integrating catalytic hairpin assembly (CHA) with DNA tile self‐assembly technology to achieve caveolae‐mediated endocytosis for the amplified imaging of miRNAs in a complex intracellular environment. Compared with classical CHA, the 9‐tile nanoarrays possess high sensitivity and specificity for miRNAs, achieve excellent internalization efficiency by caveolar endocytosis, bypassing lysosomal traps, and exhibit more powerful signal‐amplified imaging of intracellular miRNAs. Because of their excellent safety, physiological stability, and highly efficient cytoplasmic delivery, the 9‐tile nanoarrays can realize real‐time amplified monitoring of miRNAs in various tumor and identical cells of different periods, and imaging effects are consistent with the actual expression levels of miRNAs, ultimately demonstrating their feasibility and capacity. This strategy provides a high‐potential delivery pathway for cell imaging and targeted delivery, simultaneously offering a meaningful reference for the application of DNA tile self‐assembly technology in relevant fundamental research and medical diagnostics.https://doi.org/10.1002/advs.202300614caveolae‐mediated endocytosisDNA nanostructuresDNA tile self‐assembly technologyliving cellsmicroRNAssignal‐amplified imaging |
| spellingShingle | Yanan Peng Zhijun Gao Bin Qiao Dongxia Li Huajie Pang Xiangde Lai Qiumei Pu Rui Zhang Xuan Zhao Guangyuan Zhao Dan Xu Yuanyuan Wang Yuxiang Ji Hua Pei Qiang Wu Size‐Controlled DNA Tile Self‐Assembly Nanostructures Through Caveolae‐Mediated Endocytosis for Signal‐Amplified Imaging of MicroRNAs in Living Cells Advanced Science caveolae‐mediated endocytosis DNA nanostructures DNA tile self‐assembly technology living cells microRNAs signal‐amplified imaging |
| title | Size‐Controlled DNA Tile Self‐Assembly Nanostructures Through Caveolae‐Mediated Endocytosis for Signal‐Amplified Imaging of MicroRNAs in Living Cells |
| title_full | Size‐Controlled DNA Tile Self‐Assembly Nanostructures Through Caveolae‐Mediated Endocytosis for Signal‐Amplified Imaging of MicroRNAs in Living Cells |
| title_fullStr | Size‐Controlled DNA Tile Self‐Assembly Nanostructures Through Caveolae‐Mediated Endocytosis for Signal‐Amplified Imaging of MicroRNAs in Living Cells |
| title_full_unstemmed | Size‐Controlled DNA Tile Self‐Assembly Nanostructures Through Caveolae‐Mediated Endocytosis for Signal‐Amplified Imaging of MicroRNAs in Living Cells |
| title_short | Size‐Controlled DNA Tile Self‐Assembly Nanostructures Through Caveolae‐Mediated Endocytosis for Signal‐Amplified Imaging of MicroRNAs in Living Cells |
| title_sort | size controlled dna tile self assembly nanostructures through caveolae mediated endocytosis for signal amplified imaging of micrornas in living cells |
| topic | caveolae‐mediated endocytosis DNA nanostructures DNA tile self‐assembly technology living cells microRNAs signal‐amplified imaging |
| url | https://doi.org/10.1002/advs.202300614 |
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