Estradiol valerate improves endometrial receptivity induced by clomiphene in rat with polycystic ovary syndrome
Objective To investigate the effect of estradiol valerate (E2V) on endometrial receptivity induced by clomiphene in polycystic ovary syndrome (PCOS) rats. Methods Rats were divided into control group (administered 0.9% sodium chloride solution), and PCOS model group (administered 50 mg/kg clomiphene...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2020-01-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a181222.pdf |
| _version_ | 1797365725334077440 |
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| author | SHAO Rui-feng, HE Chi-hua, WANG Ying |
| author_facet | SHAO Rui-feng, HE Chi-hua, WANG Ying |
| author_sort | SHAO Rui-feng, HE Chi-hua, WANG Ying |
| collection | DOAJ |
| description | Objective To investigate the effect of estradiol valerate (E2V) on endometrial receptivity induced by clomiphene in polycystic ovary syndrome (PCOS) rats. Methods Rats were divided into control group (administered 0.9% sodium chloride solution), and PCOS model group (administered 50 mg/kg clomiphene), E2V low, medium and high-dose intervention group [administered (0.2, 0.5, 1.0)mg/kg E2V+50 mg/kg clomiphene]. Serum sex hormone level was compared. Morphological change of endometrium was examined by ELISA. The expression of estrogen receptor (ER), progesterone receptor (PR), homeobox gene (Hox)A10, empty ventilator homeobox (Emx)2, integrin αvβ3 mRNA and protein in the endometrium were detected by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and Western blot. Results Compared with the control group, luteinizing hormone/follicle-forming hormone (LH/FSH), testosterone (T), relative expressions of Emx2 mRNA and protein were significantly increased (P＜0.05), while estradiol (E2) level, glandular area, glandular area, endometrial thickness and glandular/interstitial area ratio, relative expressions of ER, PR, HoxA10, integrin αvβ3 mRNA and protein were significantly decreased (P＜0.05). Compared with PCOS model group, the changes of above indexes in E2V low, medium and high-dose intervention group were opposite (P＜0.05). Conclusions E2V can improve endometrial receptivity of PCOS clomiphene-induced ovulation rats. E2V is potentially related to the up-regulation of ER, PR, HoxA10, integrinαvβ3 and down-regulation of Emx2. |
| first_indexed | 2024-03-08T16:54:00Z |
| format | Article |
| id | doaj.art-7da2e7285bcc4555bdd4a09df063e0ae |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:54:00Z |
| publishDate | 2020-01-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-7da2e7285bcc4555bdd4a09df063e0ae2024-01-05T03:13:53ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252020-01-014017882Estradiol valerate improves endometrial receptivity induced by clomiphene in rat with polycystic ovary syndromeSHAO Rui-feng, HE Chi-hua, WANG Ying0Department of Reproductive Medicine, Jingzhou Central Hospital, Jingzhou 434000, ChinaObjective To investigate the effect of estradiol valerate (E2V) on endometrial receptivity induced by clomiphene in polycystic ovary syndrome (PCOS) rats. Methods Rats were divided into control group (administered 0.9% sodium chloride solution), and PCOS model group (administered 50 mg/kg clomiphene), E2V low, medium and high-dose intervention group [administered (0.2, 0.5, 1.0)mg/kg E2V+50 mg/kg clomiphene]. Serum sex hormone level was compared. Morphological change of endometrium was examined by ELISA. The expression of estrogen receptor (ER), progesterone receptor (PR), homeobox gene (Hox)A10, empty ventilator homeobox (Emx)2, integrin αvβ3 mRNA and protein in the endometrium were detected by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and Western blot. Results Compared with the control group, luteinizing hormone/follicle-forming hormone (LH/FSH), testosterone (T), relative expressions of Emx2 mRNA and protein were significantly increased (P＜0.05), while estradiol (E2) level, glandular area, glandular area, endometrial thickness and glandular/interstitial area ratio, relative expressions of ER, PR, HoxA10, integrin αvβ3 mRNA and protein were significantly decreased (P＜0.05). Compared with PCOS model group, the changes of above indexes in E2V low, medium and high-dose intervention group were opposite (P＜0.05). Conclusions E2V can improve endometrial receptivity of PCOS clomiphene-induced ovulation rats. E2V is potentially related to the up-regulation of ER, PR, HoxA10, integrinαvβ3 and down-regulation of Emx2.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a181222.pdfestradiol valerate|polycystic ovary syndrome|endometrial receptivity |
| spellingShingle | SHAO Rui-feng, HE Chi-hua, WANG Ying Estradiol valerate improves endometrial receptivity induced by clomiphene in rat with polycystic ovary syndrome Jichu yixue yu linchuang estradiol valerate|polycystic ovary syndrome|endometrial receptivity |
| title | Estradiol valerate improves endometrial receptivity induced by clomiphene in rat with polycystic ovary syndrome |
| title_full | Estradiol valerate improves endometrial receptivity induced by clomiphene in rat with polycystic ovary syndrome |
| title_fullStr | Estradiol valerate improves endometrial receptivity induced by clomiphene in rat with polycystic ovary syndrome |
| title_full_unstemmed | Estradiol valerate improves endometrial receptivity induced by clomiphene in rat with polycystic ovary syndrome |
| title_short | Estradiol valerate improves endometrial receptivity induced by clomiphene in rat with polycystic ovary syndrome |
| title_sort | estradiol valerate improves endometrial receptivity induced by clomiphene in rat with polycystic ovary syndrome |
| topic | estradiol valerate|polycystic ovary syndrome|endometrial receptivity |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a181222.pdf |
| work_keys_str_mv | AT shaoruifenghechihuawangying estradiolvalerateimprovesendometrialreceptivityinducedbyclomipheneinratwithpolycysticovarysyndrome |