Nicotinamide Mononucleotide Potentiates Resistance to Biotrophic Invasion of Fungal Pathogens in Barley
Nicotinamide mononucleotide (NMN), a precursor of nicotinamide adenine dinucleotide (NAD), induces disease resistance to the Fusarium head blight fungus <i>Fusarium graminearum</i> in <i>Arabidopsis</i> and barley, but it is unknown at which stage of the infection it acts. Si...
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/5/2696 |
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| author | Kana Ueda Yuichi Nakajima Hiroshi Inoue Kappei Kobayashi Takumi Nishiuchi Makoto Kimura Takashi Yaeno |
| author_facet | Kana Ueda Yuichi Nakajima Hiroshi Inoue Kappei Kobayashi Takumi Nishiuchi Makoto Kimura Takashi Yaeno |
| author_sort | Kana Ueda |
| collection | DOAJ |
| description | Nicotinamide mononucleotide (NMN), a precursor of nicotinamide adenine dinucleotide (NAD), induces disease resistance to the Fusarium head blight fungus <i>Fusarium graminearum</i> in <i>Arabidopsis</i> and barley, but it is unknown at which stage of the infection it acts. Since the rate of haustorial formation of an obligate biotrophic barley powdery mildew fungus <i>Blumeria graminis</i> f. sp. <i>hordei</i> (<i>Bgh</i>) was significantly reduced in NMN-treated coleoptile epidermal cells, the possibility that NMN induces resistance to the biotrophic stage of <i>F. graminearum</i> was investigated. The results show that NMN treatment caused the wandering of hyphal growth and suppressed the formation of appressoria-like structures. Furthermore, we developed an experimental system to monitor the early stage of infection in real-time and analyzed the infection behavior. We observed that the hyphae elongated windingly by NMN treatment. These results suggest that NMN potentiates resistance to the biotrophic invasion of <i>F. graminearum</i> as well as <i>Bgh</i>. |
| first_indexed | 2024-03-09T05:06:04Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T05:06:04Z |
| publishDate | 2021-03-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7da60bdb4c194dfcae661cf3a95c19682023-12-03T12:54:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01225269610.3390/ijms22052696Nicotinamide Mononucleotide Potentiates Resistance to Biotrophic Invasion of Fungal Pathogens in BarleyKana Ueda0Yuichi Nakajima1Hiroshi Inoue2Kappei Kobayashi3Takumi Nishiuchi4Makoto Kimura5Takashi Yaeno6Department of Agriculture, Ehime University, Tarumi, Matsuyama, Ehime 790-8566, JapanDivision of Molecular and Cellular Biology, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya, Aichi 464-8601, JapanDepartment of Agriculture, Ehime University, Tarumi, Matsuyama, Ehime 790-8566, JapanDepartment of Agriculture, Ehime University, Tarumi, Matsuyama, Ehime 790-8566, JapanInstitution for Gene Research, Advanced Science Research Centre, Kanazawa University, 13-1 Takara-machi, Kanazawa, Ishikawa 920-0934, JapanDivision of Molecular and Cellular Biology, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya, Aichi 464-8601, JapanDepartment of Agriculture, Ehime University, Tarumi, Matsuyama, Ehime 790-8566, JapanNicotinamide mononucleotide (NMN), a precursor of nicotinamide adenine dinucleotide (NAD), induces disease resistance to the Fusarium head blight fungus <i>Fusarium graminearum</i> in <i>Arabidopsis</i> and barley, but it is unknown at which stage of the infection it acts. Since the rate of haustorial formation of an obligate biotrophic barley powdery mildew fungus <i>Blumeria graminis</i> f. sp. <i>hordei</i> (<i>Bgh</i>) was significantly reduced in NMN-treated coleoptile epidermal cells, the possibility that NMN induces resistance to the biotrophic stage of <i>F. graminearum</i> was investigated. The results show that NMN treatment caused the wandering of hyphal growth and suppressed the formation of appressoria-like structures. Furthermore, we developed an experimental system to monitor the early stage of infection in real-time and analyzed the infection behavior. We observed that the hyphae elongated windingly by NMN treatment. These results suggest that NMN potentiates resistance to the biotrophic invasion of <i>F. graminearum</i> as well as <i>Bgh</i>.https://www.mdpi.com/1422-0067/22/5/2696nicotinamide mononucleotide<i>Fusarium graminearum</i><i>Blumeria graminis</i> f. sp. <i>hordei</i> |
| spellingShingle | Kana Ueda Yuichi Nakajima Hiroshi Inoue Kappei Kobayashi Takumi Nishiuchi Makoto Kimura Takashi Yaeno Nicotinamide Mononucleotide Potentiates Resistance to Biotrophic Invasion of Fungal Pathogens in Barley International Journal of Molecular Sciences nicotinamide mononucleotide <i>Fusarium graminearum</i> <i>Blumeria graminis</i> f. sp. <i>hordei</i> |
| title | Nicotinamide Mononucleotide Potentiates Resistance to Biotrophic Invasion of Fungal Pathogens in Barley |
| title_full | Nicotinamide Mononucleotide Potentiates Resistance to Biotrophic Invasion of Fungal Pathogens in Barley |
| title_fullStr | Nicotinamide Mononucleotide Potentiates Resistance to Biotrophic Invasion of Fungal Pathogens in Barley |
| title_full_unstemmed | Nicotinamide Mononucleotide Potentiates Resistance to Biotrophic Invasion of Fungal Pathogens in Barley |
| title_short | Nicotinamide Mononucleotide Potentiates Resistance to Biotrophic Invasion of Fungal Pathogens in Barley |
| title_sort | nicotinamide mononucleotide potentiates resistance to biotrophic invasion of fungal pathogens in barley |
| topic | nicotinamide mononucleotide <i>Fusarium graminearum</i> <i>Blumeria graminis</i> f. sp. <i>hordei</i> |
| url | https://www.mdpi.com/1422-0067/22/5/2696 |
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