Zika viral proteome analysis reveals an epitope cluster within NS3 helicase as a potential vaccine candidate: An in silico study
Zika virus (ZIKV), a mosquito-borne flavivirus, is now an emerging global public health concern. Currently, the pathogenicity, genetic diversity and the consequences of ZIKV infection are little known and a protective vaccine against ZIKV is urgently needed. In this study, we have taken an immunoinf...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-01-01
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| Series: | Informatics in Medicine Unlocked |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352914820305840 |
| _version_ | 1818390281442033664 |
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| author | Md Tangigul Haque Md Nur Ahad Shah Shatabdi Paul Md Kawsar Khan Payal Barua |
| author_facet | Md Tangigul Haque Md Nur Ahad Shah Shatabdi Paul Md Kawsar Khan Payal Barua |
| author_sort | Md Tangigul Haque |
| collection | DOAJ |
| description | Zika virus (ZIKV), a mosquito-borne flavivirus, is now an emerging global public health concern. Currently, the pathogenicity, genetic diversity and the consequences of ZIKV infection are little known and a protective vaccine against ZIKV is urgently needed. In this study, we have taken an immunoinformatics approach to predict epitope cluster regions in the whole proteome (3423 amino acids) of ZIKV. We used a range of bioinformatics algorithms to determine epitopes of CD8+ cytotoxic T-cells (CTL), CD4+ helper T-cells (THL) and B cells. We have predicted an epitope cluster of 23 contiguous amino acids (region 1989–2011, WLEARMLLDNIYLQDGLIASLYR) residing on the NS3 helicase protein in the ZIKV proteome. This epitope cluster contains fourteen CD4+ (THL) epitopes and six CD8+ (CTL) epitopes. Finally, we have validated the epitopes by analysing their binding efficiency (binding energy within −4.7 to −6.9 kcal/mol) with specific HLA alleles. The epitope cluster is predicted to provide 93.86% population coverage worldwide. Based on our immunoinformatics analysis, we propose the peptide WLEARMLLDNIYLQDGLIASLYR as a new vaccine candidate against Zika virus for further validation. |
| first_indexed | 2024-12-14T04:55:08Z |
| format | Article |
| id | doaj.art-7da8e65831974e0fb2ea2820bf6498e0 |
| institution | Directory Open Access Journal |
| issn | 2352-9148 |
| language | English |
| last_indexed | 2024-12-14T04:55:08Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Informatics in Medicine Unlocked |
| spelling | doaj.art-7da8e65831974e0fb2ea2820bf6498e02022-12-21T23:16:25ZengElsevierInformatics in Medicine Unlocked2352-91482020-01-0121100434Zika viral proteome analysis reveals an epitope cluster within NS3 helicase as a potential vaccine candidate: An in silico studyMd Tangigul Haque0Md Nur Ahad Shah1Shatabdi Paul2Md Kawsar Khan3Payal Barua4Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet, 3114, Bangladesh; Department of Biochemistry, Primeasia University, Dhaka, BangladeshDepartment of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet, 3114, BangladeshDepartment of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet, 3114, Bangladesh; Department of Biochemistry, Primeasia University, Dhaka, BangladeshDepartment of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet, 3114, Bangladesh; Department of Biological Sciences, Macquarie University, NSW, 2109, AustraliaDepartment of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet, 3114, Bangladesh; Corresponding author.Zika virus (ZIKV), a mosquito-borne flavivirus, is now an emerging global public health concern. Currently, the pathogenicity, genetic diversity and the consequences of ZIKV infection are little known and a protective vaccine against ZIKV is urgently needed. In this study, we have taken an immunoinformatics approach to predict epitope cluster regions in the whole proteome (3423 amino acids) of ZIKV. We used a range of bioinformatics algorithms to determine epitopes of CD8+ cytotoxic T-cells (CTL), CD4+ helper T-cells (THL) and B cells. We have predicted an epitope cluster of 23 contiguous amino acids (region 1989–2011, WLEARMLLDNIYLQDGLIASLYR) residing on the NS3 helicase protein in the ZIKV proteome. This epitope cluster contains fourteen CD4+ (THL) epitopes and six CD8+ (CTL) epitopes. Finally, we have validated the epitopes by analysing their binding efficiency (binding energy within −4.7 to −6.9 kcal/mol) with specific HLA alleles. The epitope cluster is predicted to provide 93.86% population coverage worldwide. Based on our immunoinformatics analysis, we propose the peptide WLEARMLLDNIYLQDGLIASLYR as a new vaccine candidate against Zika virus for further validation.http://www.sciencedirect.com/science/article/pii/S2352914820305840EpitopeImmunoinformaticsVaccineZika virusProteome |
| spellingShingle | Md Tangigul Haque Md Nur Ahad Shah Shatabdi Paul Md Kawsar Khan Payal Barua Zika viral proteome analysis reveals an epitope cluster within NS3 helicase as a potential vaccine candidate: An in silico study Informatics in Medicine Unlocked Epitope Immunoinformatics Vaccine Zika virus Proteome |
| title | Zika viral proteome analysis reveals an epitope cluster within NS3 helicase as a potential vaccine candidate: An in silico study |
| title_full | Zika viral proteome analysis reveals an epitope cluster within NS3 helicase as a potential vaccine candidate: An in silico study |
| title_fullStr | Zika viral proteome analysis reveals an epitope cluster within NS3 helicase as a potential vaccine candidate: An in silico study |
| title_full_unstemmed | Zika viral proteome analysis reveals an epitope cluster within NS3 helicase as a potential vaccine candidate: An in silico study |
| title_short | Zika viral proteome analysis reveals an epitope cluster within NS3 helicase as a potential vaccine candidate: An in silico study |
| title_sort | zika viral proteome analysis reveals an epitope cluster within ns3 helicase as a potential vaccine candidate an in silico study |
| topic | Epitope Immunoinformatics Vaccine Zika virus Proteome |
| url | http://www.sciencedirect.com/science/article/pii/S2352914820305840 |
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