Optimising aerosol jet printing of collagen inks for enhanced piezoelectricity and controlled surface potential
Collagen is a highly versatile protein used in tissue engineering constructs and as a model piezoelectric biomaterial. The piezoelectricity of collagen can be enhanced through the alignment of collagen domains and fibres, although most fabrication techniques used to form dense collagenous constructs...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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IOP Publishing
2023-01-01
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| Series: | JPhys Materials |
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| Online Access: | https://doi.org/10.1088/2515-7639/acca4a |
| _version_ | 1797834365844062208 |
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| author | Malavika Nair Ella Inwald Liam Ives Kirsten R M See Sohini Kar-Narayan |
| author_facet | Malavika Nair Ella Inwald Liam Ives Kirsten R M See Sohini Kar-Narayan |
| author_sort | Malavika Nair |
| collection | DOAJ |
| description | Collagen is a highly versatile protein used in tissue engineering constructs and as a model piezoelectric biomaterial. The piezoelectricity of collagen can be enhanced through the alignment of collagen domains and fibres, although most fabrication techniques used to form dense collagenous constructs do not allow for significant collagen alignment. The use of aerosol jet printing (AJP) mitigates the limitations of using soluble collagen inks for bioprinting or extrusion-based 3D printing, particularly if microfibrillar collagen suspensions are used as a cost-effective and scalable ink source. In this work, Type I and Type II microfibrillar collagen from different anatomical sources were successfully deposited using AJP with two different atomisation methods, namely pneumatic-AJP (p-AJP) and ultrasonic-AJP (u-AJP). The printing parameters were optimised for their piezoelectric amplitude and surface potential. Fourier transform infrared spectra of the films revealed that ultrasonic atomisation did not cause notable denaturation of collagen, although the process resulted in the fractionation and preferential deposition of the oligomeric and gelatinous components within the slurry. The printed collagen samples displayed a piezoelectric response that was four times higher than the values obtained from drop-cast collagen films, and their surface potential was found to be positively correlated to the roughness of the films which can be controlled through the mode of atomisation. These results indicate the ability to enhance and control the piezoelectricity and surface potential using p-AJP and u-AJP, which offers a promising physical modulation technique to tailor cell adhesion, proliferation or differentiation for collagen-based tissue engineering constructs. |
| first_indexed | 2024-04-09T14:36:59Z |
| format | Article |
| id | doaj.art-7daa1d59d9944e08b89f61ca11eeee51 |
| institution | Directory Open Access Journal |
| issn | 2515-7639 |
| language | English |
| last_indexed | 2024-04-09T14:36:59Z |
| publishDate | 2023-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | JPhys Materials |
| spelling | doaj.art-7daa1d59d9944e08b89f61ca11eeee512023-05-03T12:00:35ZengIOP PublishingJPhys Materials2515-76392023-01-016303400110.1088/2515-7639/acca4aOptimising aerosol jet printing of collagen inks for enhanced piezoelectricity and controlled surface potentialMalavika Nair0https://orcid.org/0000-0002-5229-8991Ella Inwald1Liam Ives2https://orcid.org/0000-0001-8705-7269Kirsten R M See3Sohini Kar-Narayan4https://orcid.org/0000-0002-8151-1616Department of Materials Science and Metallurgy, University of Cambridge , 27 Charles Babbage Road, Cambridge CB3 0FS, United Kingdom; Institute of Biomedical Engineering, University of Oxford , Marcela Botnar Wing, Windmill Road, Oxford, OX3 7LD, United KingdomDepartment of Materials Science and Metallurgy, University of Cambridge , 27 Charles Babbage Road, Cambridge CB3 0FS, United KingdomDepartment of Materials Science and Metallurgy, University of Cambridge , 27 Charles Babbage Road, Cambridge CB3 0FS, United KingdomDepartment of Materials Science and Metallurgy, University of Cambridge , 27 Charles Babbage Road, Cambridge CB3 0FS, United KingdomDepartment of Materials Science and Metallurgy, University of Cambridge , 27 Charles Babbage Road, Cambridge CB3 0FS, United KingdomCollagen is a highly versatile protein used in tissue engineering constructs and as a model piezoelectric biomaterial. The piezoelectricity of collagen can be enhanced through the alignment of collagen domains and fibres, although most fabrication techniques used to form dense collagenous constructs do not allow for significant collagen alignment. The use of aerosol jet printing (AJP) mitigates the limitations of using soluble collagen inks for bioprinting or extrusion-based 3D printing, particularly if microfibrillar collagen suspensions are used as a cost-effective and scalable ink source. In this work, Type I and Type II microfibrillar collagen from different anatomical sources were successfully deposited using AJP with two different atomisation methods, namely pneumatic-AJP (p-AJP) and ultrasonic-AJP (u-AJP). The printing parameters were optimised for their piezoelectric amplitude and surface potential. Fourier transform infrared spectra of the films revealed that ultrasonic atomisation did not cause notable denaturation of collagen, although the process resulted in the fractionation and preferential deposition of the oligomeric and gelatinous components within the slurry. The printed collagen samples displayed a piezoelectric response that was four times higher than the values obtained from drop-cast collagen films, and their surface potential was found to be positively correlated to the roughness of the films which can be controlled through the mode of atomisation. These results indicate the ability to enhance and control the piezoelectricity and surface potential using p-AJP and u-AJP, which offers a promising physical modulation technique to tailor cell adhesion, proliferation or differentiation for collagen-based tissue engineering constructs.https://doi.org/10.1088/2515-7639/acca4aaerosol jet printingcollagenpiezoelectricitysurface potential |
| spellingShingle | Malavika Nair Ella Inwald Liam Ives Kirsten R M See Sohini Kar-Narayan Optimising aerosol jet printing of collagen inks for enhanced piezoelectricity and controlled surface potential JPhys Materials aerosol jet printing collagen piezoelectricity surface potential |
| title | Optimising aerosol jet printing of collagen inks for enhanced piezoelectricity and controlled surface potential |
| title_full | Optimising aerosol jet printing of collagen inks for enhanced piezoelectricity and controlled surface potential |
| title_fullStr | Optimising aerosol jet printing of collagen inks for enhanced piezoelectricity and controlled surface potential |
| title_full_unstemmed | Optimising aerosol jet printing of collagen inks for enhanced piezoelectricity and controlled surface potential |
| title_short | Optimising aerosol jet printing of collagen inks for enhanced piezoelectricity and controlled surface potential |
| title_sort | optimising aerosol jet printing of collagen inks for enhanced piezoelectricity and controlled surface potential |
| topic | aerosol jet printing collagen piezoelectricity surface potential |
| url | https://doi.org/10.1088/2515-7639/acca4a |
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