Botryosphaeria spp. as Producers of Laccase When Cultivated on Vegetable Oils as Sole Carbon Source: Optimizing Laccase Production by Botryosphaeria rhodina MAMB-05 on Soybean Oil
<p class="orbitalabstract">Eight vegetable oils and glycerol were examined as sole carbon source to screen nine isolates of Botryosphaeria spp. Among these, B. rhodina MAMB-05 produced highest laccase titres on all carbon sources evaluated followed by B. rhodina MMLR isolated (from o...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Mato Grosso do Sul
2018-12-01
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| Series: | Orbital: The Electronic Journal of Chemistry |
| Subjects: | |
| Online Access: | http://orbital.ufms.br/index.php/Chemistry/article/view/1206 |
| _version_ | 1818294185362456576 |
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| author | João Paulo Sacchetto Arnaldo F. Da Silva Filho Maria Inês Rezende Ieda S. Scarmínio Marco A. Z. Ayub Ellen Cristine Giese Robert F. H. Dekker Aneli Melo Barbosa-Dekker |
| author_facet | João Paulo Sacchetto Arnaldo F. Da Silva Filho Maria Inês Rezende Ieda S. Scarmínio Marco A. Z. Ayub Ellen Cristine Giese Robert F. H. Dekker Aneli Melo Barbosa-Dekker |
| author_sort | João Paulo Sacchetto |
| collection | DOAJ |
| description | <p class="orbitalabstract">Eight vegetable oils and glycerol were examined as sole carbon source to screen nine isolates of Botryosphaeria spp. Among these, B. rhodina MAMB-05 produced highest laccase titres on all carbon sources evaluated followed by B. rhodina MMLR isolated (from orange, Citrus sp.). The influence of inorganic nitrogen (NH<sub>4</sub>Cl, NaNO<sub>3</sub>, (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>, and NH<sub>4</sub>NO<sub>3</sub>), organic nitrogen (yeast extract (YE), urea, peptone, and corn steep liquor) sources, inorganic phosphate (KH<sub>2</sub>PO<sub>4</sub>, Na<sub>2</sub>HPO<sub>4</sub>, NaH<sub>2</sub>PO<sub>4</sub>, K<sub>2</sub>HPO<sub>4</sub>), as well as increasing concentrations of copper (from zero to 100 µg mL<sup>-1</sup>), as nutritional requirements for enhancing laccase production by B. rhodina MAMB-05 were also evaluated. Soybean oil was the only carbon source in a central composite factorialdesign (2<sup>3</sup>) to optimize laccase production with the selected variables NaNO<sub>3</sub>, KH<sub>2</sub>PO<sub>4</sub> and copper (CuSO<sub>4</sub>.5H<sub>2</sub>O), using the response surface methodology. The optimal conditions obtained were NaNO<sub>3</sub> (100 g L<sup>-1</sup>), KH<sub>2</sub>PO<sub>4</sub> (125 g L<sup>-1</sup>) and 50 µg mL<sup>-1</sup> of CuSO<sub>4</sub>.5H<sub>2</sub>O. The specific laccase activity obtained (2.95 U mg<sup>-1</sup>) in the validation experiments using the optimal condition for laccase production by B. rhodina MAMB-05 was in agreement with the predicted value (3.07 U mg<sup>-1</sup>), and demonstrated the fitness of the proposed model.</p><p class="orbitalabstract"> </p><p class="orbitalabstract">DOI: <a href="http://dx.doi.org/10.17807/orbital.v10i7.1206">http://dx.doi.org/10.17807/orbital.v10i7.1206</a></p> <p class="orbitalabstract"> </p> |
| first_indexed | 2024-12-13T03:27:44Z |
| format | Article |
| id | doaj.art-7daa487f4e5e4a78a1e8ea925934e69b |
| institution | Directory Open Access Journal |
| issn | 1984-6428 |
| language | English |
| last_indexed | 2024-12-13T03:27:44Z |
| publishDate | 2018-12-01 |
| publisher | Universidade Federal de Mato Grosso do Sul |
| record_format | Article |
| series | Orbital: The Electronic Journal of Chemistry |
| spelling | doaj.art-7daa487f4e5e4a78a1e8ea925934e69b2022-12-22T00:01:14ZengUniversidade Federal de Mato Grosso do SulOrbital: The Electronic Journal of Chemistry1984-64282018-12-0110756857710.17807/orbital.v10i7.1206534Botryosphaeria spp. as Producers of Laccase When Cultivated on Vegetable Oils as Sole Carbon Source: Optimizing Laccase Production by Botryosphaeria rhodina MAMB-05 on Soybean OilJoão Paulo Sacchetto0Arnaldo F. Da Silva Filho1Maria Inês Rezende2Ieda S. Scarmínio3Marco A. Z. AyubEllen Cristine GieseRobert F. H. DekkerAneli Melo Barbosa-Dekker4Centro de Tecnologia MineralUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de Londrina<p class="orbitalabstract">Eight vegetable oils and glycerol were examined as sole carbon source to screen nine isolates of Botryosphaeria spp. Among these, B. rhodina MAMB-05 produced highest laccase titres on all carbon sources evaluated followed by B. rhodina MMLR isolated (from orange, Citrus sp.). The influence of inorganic nitrogen (NH<sub>4</sub>Cl, NaNO<sub>3</sub>, (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>, and NH<sub>4</sub>NO<sub>3</sub>), organic nitrogen (yeast extract (YE), urea, peptone, and corn steep liquor) sources, inorganic phosphate (KH<sub>2</sub>PO<sub>4</sub>, Na<sub>2</sub>HPO<sub>4</sub>, NaH<sub>2</sub>PO<sub>4</sub>, K<sub>2</sub>HPO<sub>4</sub>), as well as increasing concentrations of copper (from zero to 100 µg mL<sup>-1</sup>), as nutritional requirements for enhancing laccase production by B. rhodina MAMB-05 were also evaluated. Soybean oil was the only carbon source in a central composite factorialdesign (2<sup>3</sup>) to optimize laccase production with the selected variables NaNO<sub>3</sub>, KH<sub>2</sub>PO<sub>4</sub> and copper (CuSO<sub>4</sub>.5H<sub>2</sub>O), using the response surface methodology. The optimal conditions obtained were NaNO<sub>3</sub> (100 g L<sup>-1</sup>), KH<sub>2</sub>PO<sub>4</sub> (125 g L<sup>-1</sup>) and 50 µg mL<sup>-1</sup> of CuSO<sub>4</sub>.5H<sub>2</sub>O. The specific laccase activity obtained (2.95 U mg<sup>-1</sup>) in the validation experiments using the optimal condition for laccase production by B. rhodina MAMB-05 was in agreement with the predicted value (3.07 U mg<sup>-1</sup>), and demonstrated the fitness of the proposed model.</p><p class="orbitalabstract"> </p><p class="orbitalabstract">DOI: <a href="http://dx.doi.org/10.17807/orbital.v10i7.1206">http://dx.doi.org/10.17807/orbital.v10i7.1206</a></p> <p class="orbitalabstract"> </p>http://orbital.ufms.br/index.php/Chemistry/article/view/1206botryosphaeria rhodina mamb-05laccasesoybean oil, glycerolresponse surface methodology |
| spellingShingle | João Paulo Sacchetto Arnaldo F. Da Silva Filho Maria Inês Rezende Ieda S. Scarmínio Marco A. Z. Ayub Ellen Cristine Giese Robert F. H. Dekker Aneli Melo Barbosa-Dekker Botryosphaeria spp. as Producers of Laccase When Cultivated on Vegetable Oils as Sole Carbon Source: Optimizing Laccase Production by Botryosphaeria rhodina MAMB-05 on Soybean Oil Orbital: The Electronic Journal of Chemistry botryosphaeria rhodina mamb-05 laccase soybean oil, glycerol response surface methodology |
| title | Botryosphaeria spp. as Producers of Laccase When Cultivated on Vegetable Oils as Sole Carbon Source: Optimizing Laccase Production by Botryosphaeria rhodina MAMB-05 on Soybean Oil |
| title_full | Botryosphaeria spp. as Producers of Laccase When Cultivated on Vegetable Oils as Sole Carbon Source: Optimizing Laccase Production by Botryosphaeria rhodina MAMB-05 on Soybean Oil |
| title_fullStr | Botryosphaeria spp. as Producers of Laccase When Cultivated on Vegetable Oils as Sole Carbon Source: Optimizing Laccase Production by Botryosphaeria rhodina MAMB-05 on Soybean Oil |
| title_full_unstemmed | Botryosphaeria spp. as Producers of Laccase When Cultivated on Vegetable Oils as Sole Carbon Source: Optimizing Laccase Production by Botryosphaeria rhodina MAMB-05 on Soybean Oil |
| title_short | Botryosphaeria spp. as Producers of Laccase When Cultivated on Vegetable Oils as Sole Carbon Source: Optimizing Laccase Production by Botryosphaeria rhodina MAMB-05 on Soybean Oil |
| title_sort | botryosphaeria spp as producers of laccase when cultivated on vegetable oils as sole carbon source optimizing laccase production by botryosphaeria rhodina mamb 05 on soybean oil |
| topic | botryosphaeria rhodina mamb-05 laccase soybean oil, glycerol response surface methodology |
| url | http://orbital.ufms.br/index.php/Chemistry/article/view/1206 |
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