Plant growth-promoting rhizobacteria Burkholderia vietnamiensis B418 inhibits root-knot nematode on watermelon by modifying the rhizosphere microbial community
Abstract Burkholderia vietnamiensis B418 is a multifunctional plant growth-promoting rhizobacteria (PGPR) strain with nitrogen-fixing and phosphate-solubilizing capability which can be employed for root-knot nematode (RKN) management on various crops and vegetables. Here we investigated the control...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-05-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-12472-2 |
| _version_ | 1818554758054543360 |
|---|---|
| author | Minmin Liu Joshua Philp Yilian Wang Jindong Hu Yanli Wei Jishun Li Maarten Ryder Ruey Toh Yi Zhou Matthew D. Denton Yuanzheng Wu Hetong Yang |
| author_facet | Minmin Liu Joshua Philp Yilian Wang Jindong Hu Yanli Wei Jishun Li Maarten Ryder Ruey Toh Yi Zhou Matthew D. Denton Yuanzheng Wu Hetong Yang |
| author_sort | Minmin Liu |
| collection | DOAJ |
| description | Abstract Burkholderia vietnamiensis B418 is a multifunctional plant growth-promoting rhizobacteria (PGPR) strain with nitrogen-fixing and phosphate-solubilizing capability which can be employed for root-knot nematode (RKN) management on various crops and vegetables. Here we investigated the control efficacy of B. vietnamiensis B418 inoculation against RKN on watermelon, applied either alone or combined with nematicides fosthiazate or avermectin, and their effects on bacterial and fungal microbiomes in rhizosphere soil. The results of field experiments showed individual application of B418 displayed the highest control efficacy against RKN by 71.15%. The combinations with fosthiazate and avermectin exhibited slight incompatibility with lower inhibitory effects of 62.71% and 67.87%, respectively, which were still notably higher than these nematicides applied separately. Analysis of microbiome assemblages revealed B418 inoculation resulted in a slight reduction for bacterial community and a significant increment for fungal community, suggesting that B418 could compete with other bacteria and stimulate fungal diversity in rhizosphere. The relative abundance of Xanthomonadales, Gemmatimonadales and Sphingomonadales increased while that of Actinomycetales reduced with B418 inoculation. The predominate Sordariomycetes of fungal community decreased dramatically in control treatment with B418 inoculation whereas there were increments in fosthiazate and avermectin treatments. Additionally, nitrogen (N) cycling by soil microbes was estimated by quantifying the abundance of microbial functional genes involved in N-transformation processes as B418 has the capability of N-fixation. The copy number of N-fixing gene nifH increased with B418 inoculation, and the highest increment reached 35.66% in control treatment. Our results demonstrate that B. vietnamiensis B418 is an effective biological nematicide for nematode management, which acts through the modulation of rhizosphere microbial community. |
| first_indexed | 2024-12-12T09:44:18Z |
| format | Article |
| id | doaj.art-7daa4afb80fe43d799c695df53226eac |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T09:44:18Z |
| publishDate | 2022-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7daa4afb80fe43d799c695df53226eac2022-12-22T00:28:27ZengNature PortfolioScientific Reports2045-23222022-05-0112111310.1038/s41598-022-12472-2Plant growth-promoting rhizobacteria Burkholderia vietnamiensis B418 inhibits root-knot nematode on watermelon by modifying the rhizosphere microbial communityMinmin Liu0Joshua Philp1Yilian Wang2Jindong Hu3Yanli Wei4Jishun Li5Maarten Ryder6Ruey Toh7Yi Zhou8Matthew D. Denton9Yuanzheng Wu10Hetong Yang11School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences)Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences)Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences)Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences)Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences)Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences)China-Australia Joint Laboratory for Soil Ecological Health and Remediation, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences)China-Australia Joint Laboratory for Soil Ecological Health and Remediation, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences)China-Australia Joint Laboratory for Soil Ecological Health and Remediation, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences)China-Australia Joint Laboratory for Soil Ecological Health and Remediation, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences)Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences)School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences)Abstract Burkholderia vietnamiensis B418 is a multifunctional plant growth-promoting rhizobacteria (PGPR) strain with nitrogen-fixing and phosphate-solubilizing capability which can be employed for root-knot nematode (RKN) management on various crops and vegetables. Here we investigated the control efficacy of B. vietnamiensis B418 inoculation against RKN on watermelon, applied either alone or combined with nematicides fosthiazate or avermectin, and their effects on bacterial and fungal microbiomes in rhizosphere soil. The results of field experiments showed individual application of B418 displayed the highest control efficacy against RKN by 71.15%. The combinations with fosthiazate and avermectin exhibited slight incompatibility with lower inhibitory effects of 62.71% and 67.87%, respectively, which were still notably higher than these nematicides applied separately. Analysis of microbiome assemblages revealed B418 inoculation resulted in a slight reduction for bacterial community and a significant increment for fungal community, suggesting that B418 could compete with other bacteria and stimulate fungal diversity in rhizosphere. The relative abundance of Xanthomonadales, Gemmatimonadales and Sphingomonadales increased while that of Actinomycetales reduced with B418 inoculation. The predominate Sordariomycetes of fungal community decreased dramatically in control treatment with B418 inoculation whereas there were increments in fosthiazate and avermectin treatments. Additionally, nitrogen (N) cycling by soil microbes was estimated by quantifying the abundance of microbial functional genes involved in N-transformation processes as B418 has the capability of N-fixation. The copy number of N-fixing gene nifH increased with B418 inoculation, and the highest increment reached 35.66% in control treatment. Our results demonstrate that B. vietnamiensis B418 is an effective biological nematicide for nematode management, which acts through the modulation of rhizosphere microbial community.https://doi.org/10.1038/s41598-022-12472-2 |
| spellingShingle | Minmin Liu Joshua Philp Yilian Wang Jindong Hu Yanli Wei Jishun Li Maarten Ryder Ruey Toh Yi Zhou Matthew D. Denton Yuanzheng Wu Hetong Yang Plant growth-promoting rhizobacteria Burkholderia vietnamiensis B418 inhibits root-knot nematode on watermelon by modifying the rhizosphere microbial community Scientific Reports |
| title | Plant growth-promoting rhizobacteria Burkholderia vietnamiensis B418 inhibits root-knot nematode on watermelon by modifying the rhizosphere microbial community |
| title_full | Plant growth-promoting rhizobacteria Burkholderia vietnamiensis B418 inhibits root-knot nematode on watermelon by modifying the rhizosphere microbial community |
| title_fullStr | Plant growth-promoting rhizobacteria Burkholderia vietnamiensis B418 inhibits root-knot nematode on watermelon by modifying the rhizosphere microbial community |
| title_full_unstemmed | Plant growth-promoting rhizobacteria Burkholderia vietnamiensis B418 inhibits root-knot nematode on watermelon by modifying the rhizosphere microbial community |
| title_short | Plant growth-promoting rhizobacteria Burkholderia vietnamiensis B418 inhibits root-knot nematode on watermelon by modifying the rhizosphere microbial community |
| title_sort | plant growth promoting rhizobacteria burkholderia vietnamiensis b418 inhibits root knot nematode on watermelon by modifying the rhizosphere microbial community |
| url | https://doi.org/10.1038/s41598-022-12472-2 |
| work_keys_str_mv | AT minminliu plantgrowthpromotingrhizobacteriaburkholderiavietnamiensisb418inhibitsrootknotnematodeonwatermelonbymodifyingtherhizospheremicrobialcommunity AT joshuaphilp plantgrowthpromotingrhizobacteriaburkholderiavietnamiensisb418inhibitsrootknotnematodeonwatermelonbymodifyingtherhizospheremicrobialcommunity AT yilianwang plantgrowthpromotingrhizobacteriaburkholderiavietnamiensisb418inhibitsrootknotnematodeonwatermelonbymodifyingtherhizospheremicrobialcommunity AT jindonghu plantgrowthpromotingrhizobacteriaburkholderiavietnamiensisb418inhibitsrootknotnematodeonwatermelonbymodifyingtherhizospheremicrobialcommunity AT yanliwei plantgrowthpromotingrhizobacteriaburkholderiavietnamiensisb418inhibitsrootknotnematodeonwatermelonbymodifyingtherhizospheremicrobialcommunity AT jishunli plantgrowthpromotingrhizobacteriaburkholderiavietnamiensisb418inhibitsrootknotnematodeonwatermelonbymodifyingtherhizospheremicrobialcommunity AT maartenryder plantgrowthpromotingrhizobacteriaburkholderiavietnamiensisb418inhibitsrootknotnematodeonwatermelonbymodifyingtherhizospheremicrobialcommunity AT rueytoh plantgrowthpromotingrhizobacteriaburkholderiavietnamiensisb418inhibitsrootknotnematodeonwatermelonbymodifyingtherhizospheremicrobialcommunity AT yizhou plantgrowthpromotingrhizobacteriaburkholderiavietnamiensisb418inhibitsrootknotnematodeonwatermelonbymodifyingtherhizospheremicrobialcommunity AT matthewddenton plantgrowthpromotingrhizobacteriaburkholderiavietnamiensisb418inhibitsrootknotnematodeonwatermelonbymodifyingtherhizospheremicrobialcommunity AT yuanzhengwu plantgrowthpromotingrhizobacteriaburkholderiavietnamiensisb418inhibitsrootknotnematodeonwatermelonbymodifyingtherhizospheremicrobialcommunity AT hetongyang plantgrowthpromotingrhizobacteriaburkholderiavietnamiensisb418inhibitsrootknotnematodeonwatermelonbymodifyingtherhizospheremicrobialcommunity |