In ovo feeding of probiotic lactobacilli differentially alters expression of genes involved in the development and immunological maturation of bursa of Fabricius in pre-hatched chicks
ABSTRACT: Compelling evidence indicates that immunological maturation of the gut-associated lymphoid tissues, including the bursa of Fabricius, is dependent upon antigenic stimulation post-hatch. In view of these data, the present study investigated the impact of exposing the immune system of chick...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123007563 |
| _version_ | 1827391244597198848 |
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| author | Shreeya Sharma Raveendra R. Kulkarni Shayan Sharif Hosni Hassan Mohammadali Alizadeh Scott Pratt Khaled Abdelaziz |
| author_facet | Shreeya Sharma Raveendra R. Kulkarni Shayan Sharif Hosni Hassan Mohammadali Alizadeh Scott Pratt Khaled Abdelaziz |
| author_sort | Shreeya Sharma |
| collection | DOAJ |
| description | ABSTRACT: Compelling evidence indicates that immunological maturation of the gut-associated lymphoid tissues, including the bursa of Fabricius, is dependent upon antigenic stimulation post-hatch. In view of these data, the present study investigated the impact of exposing the immune system of chick embryos to antigenic stimuli, via in ovo delivery of poultry-specific lactobacilli, on the expression of genes associated with early bursal development and maturation. Broiler line embryonated eggs were inoculated with 106 and 107 colony-forming units (CFUs) of an individual or a mixture of Lactobacillus species, including L. crispatus (C25), L. animalis (P38), L. acidophilus (P42), and L. reuteri (P43), at embryonic day 18 (ED18). The bursa of Fabricius was collected from pre-hatched chicks (ED20) to measure the expression levels of various immune system genes. The results revealed that L. acidophilus and the mixture of Lactobacillus species at the dose of 106 CFU consistently elicited higher expression of genes responsible for B cell development, differentiation, and survival (B cell activating factor (BAFF), BAFF-receptor (BAFF-R)), and antibody production (interleukin (IL)-10) and diversification (TGF-β). Similar expression patterns were also noted in T helper (Th) cell-associated cytokine genes, including Th1-type cytokines (interferon (IFN)-γ and IL-12p40), Th2-type cytokines (IL-4 and IL-13) and Th17 cytokine (IL-17). Overall, these results suggest that the supplementation of poultry-specific lactobacilli to chick embryos might be beneficial for accelerating the development and immunological maturation of the bursa of Fabricius. However, further studies are required to determine if the changes in gene expression are associated with the developmental trajectory and phenotypes of bursal cells. |
| first_indexed | 2024-03-08T17:07:48Z |
| format | Article |
| id | doaj.art-7dab0329e9464e60aade0fe3c9887417 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-08T17:07:48Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-7dab0329e9464e60aade0fe3c98874172024-01-04T04:35:43ZengElsevierPoultry Science0032-57912024-01-011031103237In ovo feeding of probiotic lactobacilli differentially alters expression of genes involved in the development and immunological maturation of bursa of Fabricius in pre-hatched chicksShreeya Sharma0Raveendra R. Kulkarni1Shayan Sharif2Hosni Hassan3Mohammadali Alizadeh4Scott Pratt5Khaled Abdelaziz6Department of Animal and Veterinary Sciences, College of Agriculture, Forestry and Life Sciences, Clemson University, Clemson, SC, USADepartment of Population Health and Pathobiology, North Carolina State University, Raleigh, NC, USADepartment of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, CanadaPrestage Department of Poultry Science, North Carolina State University, Raleigh, NC, USADepartment of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, CanadaDepartment of Animal and Veterinary Sciences, College of Agriculture, Forestry and Life Sciences, Clemson University, Clemson, SC, USADepartment of Animal and Veterinary Sciences, College of Agriculture, Forestry and Life Sciences, Clemson University, Clemson, SC, USA; Corresponding author:ABSTRACT: Compelling evidence indicates that immunological maturation of the gut-associated lymphoid tissues, including the bursa of Fabricius, is dependent upon antigenic stimulation post-hatch. In view of these data, the present study investigated the impact of exposing the immune system of chick embryos to antigenic stimuli, via in ovo delivery of poultry-specific lactobacilli, on the expression of genes associated with early bursal development and maturation. Broiler line embryonated eggs were inoculated with 106 and 107 colony-forming units (CFUs) of an individual or a mixture of Lactobacillus species, including L. crispatus (C25), L. animalis (P38), L. acidophilus (P42), and L. reuteri (P43), at embryonic day 18 (ED18). The bursa of Fabricius was collected from pre-hatched chicks (ED20) to measure the expression levels of various immune system genes. The results revealed that L. acidophilus and the mixture of Lactobacillus species at the dose of 106 CFU consistently elicited higher expression of genes responsible for B cell development, differentiation, and survival (B cell activating factor (BAFF), BAFF-receptor (BAFF-R)), and antibody production (interleukin (IL)-10) and diversification (TGF-β). Similar expression patterns were also noted in T helper (Th) cell-associated cytokine genes, including Th1-type cytokines (interferon (IFN)-γ and IL-12p40), Th2-type cytokines (IL-4 and IL-13) and Th17 cytokine (IL-17). Overall, these results suggest that the supplementation of poultry-specific lactobacilli to chick embryos might be beneficial for accelerating the development and immunological maturation of the bursa of Fabricius. However, further studies are required to determine if the changes in gene expression are associated with the developmental trajectory and phenotypes of bursal cells.http://www.sciencedirect.com/science/article/pii/S0032579123007563chickenprobioticsLactobacilluscytokinebursa of Fabricius |
| spellingShingle | Shreeya Sharma Raveendra R. Kulkarni Shayan Sharif Hosni Hassan Mohammadali Alizadeh Scott Pratt Khaled Abdelaziz In ovo feeding of probiotic lactobacilli differentially alters expression of genes involved in the development and immunological maturation of bursa of Fabricius in pre-hatched chicks Poultry Science chicken probiotics Lactobacillus cytokine bursa of Fabricius |
| title | In ovo feeding of probiotic lactobacilli differentially alters expression of genes involved in the development and immunological maturation of bursa of Fabricius in pre-hatched chicks |
| title_full | In ovo feeding of probiotic lactobacilli differentially alters expression of genes involved in the development and immunological maturation of bursa of Fabricius in pre-hatched chicks |
| title_fullStr | In ovo feeding of probiotic lactobacilli differentially alters expression of genes involved in the development and immunological maturation of bursa of Fabricius in pre-hatched chicks |
| title_full_unstemmed | In ovo feeding of probiotic lactobacilli differentially alters expression of genes involved in the development and immunological maturation of bursa of Fabricius in pre-hatched chicks |
| title_short | In ovo feeding of probiotic lactobacilli differentially alters expression of genes involved in the development and immunological maturation of bursa of Fabricius in pre-hatched chicks |
| title_sort | in ovo feeding of probiotic lactobacilli differentially alters expression of genes involved in the development and immunological maturation of bursa of fabricius in pre hatched chicks |
| topic | chicken probiotics Lactobacillus cytokine bursa of Fabricius |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123007563 |
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